Human Angiopoietin-2 ELISA Kit

Specifications

Product Data
Description	Human Angiopoietin-2 ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human Angiopoietin-2
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	156pg/ml-10,000pg/ml
Sample Type	Cell culture supernates, serum, plasma(heparin, EDTA), saliva and human milk.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human Angiopoietin-2
Sensitivity	<10pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human Angiopoietin-2 antibody 1 Lyophilized recombinant human Angiopoietin-2 standard 10ng/tubex2 Biotinylated anti- human Angiopoietin-2 antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) Sample diluent buffer 30 ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml Required But Not Provided
Background	Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin-2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation. Angiopoietin 1 binding to Tie 2 causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.
Gene Symbol	ANGPT2

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