Human Perforin ELISA Kit, 2 x 96-well (pre-coated)

Specifications

Product Data
Description	Human Perforin ELISA Kit, 2 x 96-well (pre-coated)
Size	2 x 96 wells (pre-coated)
Format	2 x 96-well (pre-coated)
Assay Type	Solid Phase Sandwich ELISA
Assay Length	2h45min
Signal	Colorimetric
Curve Range	62.5-2000 pg/ml
Sample Type	Supernatant, Buffered Solutions, Not suitable for use with serum and plasma.
Sample Volume	100 µl
Specificity	Recognizes both natural and recombinant human Perforin
Sensitivity	40 pg/ml
Cross Reactivity	No cross reactivity with Trail, TRAIL R3, TRAIL R4, Granzyme B, TNFa, IFN?, Fas, Fas L, IL-1b, IL-2
Interference	No significant interference observed with available related molecules.
Components	ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
Background	The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Gene Symbol	PRF1
Standard Curve	Representative Standard Curve

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