Human TNF alpha ELISA Kit, 2 x 96-well (pre-coated)

Specifications

Product Data
Description	Human TNF alpha ELISA Kit, 2 x 96-well (pre-coated)
Size	2 x 96 wells (pre-coated)
Format	2 x 96-well (pre-coated)
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3h40min
Signal	Colorimetric
Curve Range	25-800 pg/ml
Sample Type	Serum, Plasma, Cell culture supernatant
Sample Volume	100 µl
Specificity	Recognizes both natural and recombinant human TNF-a
Sensitivity	10 pg/ml
Cross Reactivity	No cross reactivity with other human cytokines. Cross reactivity with macac TNF-a. No interference with soluble TNFR I and TNFR II.
Components	ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
Background	This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
Gene Symbol	TNF
Standard Curve	Representative Standard Curve

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