Human IGFBP4 ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of human IGFBP4 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human IGFBP4
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	500pg/ml-32000pg/ml
Specificity	This kit is used for quantitative detection of Human IGFBP4
Sensitivity	<20pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- human IGFBP4 antibody | 1 Lyophilized recombinant human IGFBP4 standard | 32ng/tube×2 Biotinylated anti- human IGFBP4 antibody | 130μl(dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) Sample diluent buffer | 30 ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Adhesive cover | 4
Background	Insulin-like growth factor-binding protein 4 is a protein that in humans is encoded by the IGFBP4gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. In addition, IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. It is secreted by all colon cancer cells.
Gene Symbol	IGFBP4

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