Mouse Hgf ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of mouse HGF in cell culture supernates, serum and plasma(EDTA, citrate).
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for mouse HGF
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	156pg/ml-10000pg/ml
Specificity	This kit is used for quantitative detection of mouse HGF
Sensitivity	<10pg/ml
Reactivity	Mouse
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- mouse HGF antibody | 1 Lyophilized recombinant mouse HGF standard | 10ng/tube×2 Biotinylated anti- mouse HGF antibody | 130μl(dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) Sample diluent buffer | 30ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Adhesive cover | 4
Background	Hepatocyte growth factor(HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass(Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.
Gene Symbol	Hgf

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