Mouse Pdgfb ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of Mouse PDGF-BB in cell culture supernates, serum and plasma (heparin, EDTA).
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for Mouse PDGF-BB
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	62.5pg/ml-4000pg/ml
Specificity	This kit is used for quantitative detection of Mouse PDGF-BB
Sensitivity	<10pg/ml
Reactivity	Mouse
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- mouse PDGF-BB antibody | 1 Lyophilized recombinant mouse PDGF-BB standard | 10ng/tubex2 Biotinylated anti- mouse PDGF-BB antibody | 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) Sample diluent buffer | 30ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Adhesive cover | 4
Background	PDGF-BB encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Gene Symbol	Pdgfb

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