Human Heparan Sulfate Proteoglycan 2 (HSPG2) ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of human Endorepellin/HSPG2 in cell culture supernates, serum and plasma (heparin, EDTA).
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human Endorepellin/HSPG2
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	0.624ng/ml-40ng/ml
Specificity	This kit is used for quantitative detection of Human Endorepellin/HSPG2
Sensitivity	<0.05ng/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- human human Endorepellin/HSPG2 antibody | 1 Lyophilized recombinant human human Endorepellin/HSPG2 standard | 40ng/tube×2 Biotinylated anti- human human Endorepellin/HSPG2antibody | 130μl(dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) Sample diluent buffer | 30 ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Adhesive cover | 4
Background	Perlecan (PLC) also known as HSPG2, is a protein that in humans is encoded by the HSPG2 gene. It is mapped to 1p36.12. Perlecan is highly conserved across species and the available data indicate that it has evolved from ancient ancestors by gene duplication and exonshuffling. Perlecan is a key component of the vascular extracellular matrix, here it interacts with a variety of other matrix components and helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. Perlecan can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation.
Gene Symbol	HSPG2

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