Pig BDNF ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	Sandwich High Sensitivity ELISA kit for Quantitative Detection of Pig porcine BDNF. 96wells/kit, with removable strips.
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for Pig BDNF
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	31.2pg/ml-2000pg/ml
Specificity	This kit is used for quantitative detection of Pig BDNF
Sensitivity	<15pg/ml
Reactivity	Pig
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti-pig BDNF antibody 1 Lyophilized recombinant pig BDNF standard 10 ng/tube x 2 Biotinylated anti-pig BDNF antibody 100ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) 100ul (dilution 1:100) Sample diluent buffer 30ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml Plate Sealers 4 x Wash Buffer (25x) 20 ml
Background	Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult Pig porcine brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding Pig porcine BDNF to chromosome 11, band p13.
Gene Symbol	BDNF

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