Human HSD17B13 ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	Human HSD17B13 ELISA KIT (1 x 96 wells)
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	0.31ng/ml-20ng/ml
Sample Type	Human cell lysates, serumm and plasma
Sample Volume	100µl
Specificity	This kit is used for quantitative detection of Human HSD17B13
Sensitivity	0.1ng/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	HSD17B13 Antibody Coated 96-well Plate in foil pouch with desiccant|1 plate Recombinant Human HSD17B13 Standard (1000ng/ml)|0.1 mL 100x Biotinylated HumanHSD17B13 Detection Antibody|0.12 mL 100x SA-HRP Conjugate|0.12 mL Assay Buffer|60 mL Detection Antibody Diluent|12 mL Wash Buffer Concentrate 20X|60 mL Substrate|12 mL Stop Solution|12 mL
Background	17β-Hydroxysteroid dehydrogenase type 13 (HSD17B13) is an enzyme that in humans is encoded by the HSD17B13 gene. HSD17B13 is a 300-amino-acid protein that is localized on lipid droplet. Its expression is highly restricted to the liver, specifically in hepatocytes but not other cell types in the liver. HSD17B13 is involved in various metabolic processes, including steroid hormones, fatty acids, cholesterol, and bile acids. HSD17B13 has been reported to catalyze multiple substrates including steroids, bioactive lipids including leukotriene B4 and 12(R)-hydroxyeicosatetraenoic acid, and retinol. HSD17B13 enzymatic activity attributes to hepatic cell injury, inflammation, and fibrosis, chronically leading to the development of alcoholic liver disease (ALD), nonalcoholic steatohepatitis (NASH), cirrhosis, and even hepatocellular carcinoma (HCC). HSD17B13 expression is significantly upregulated in patients and mice with non-alcoholic fatty liver disease (NAFLD). Hepatic overexpression of HSD17B13 promotes lipid accumulation in the liver. It has potential as a biomarker of advanced liver disease, such as non-alcoholic steatohepatitis (NASH) and liver cancer.
Gene Symbol	HSD17B13
Standard Curve	Data image for HSD17B13 ELISA Kit.

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