Human Serum Amyloid A (SAA1) ELISA Kit 1 x 96

Specifications

Product Data
Description	Human Serum Amyloid A (SAA1) ELISA Kit 1 x 96
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich
Assay Length	4.5 hours incubations; 0.5 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	3.125ng/ml-200ng/ml
Sample Type	Human serum, plasma and other biological fluids.
Sample Volume	100µl
Specificity	This kit is used for quantitative detection of Human SAA1
Sensitivity	0.3ng/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	SAA1 Antibody Coated 96-well Plate in foil pouch with desiccant|1 plate Recombinant Human SAA1 Standard (10µg/ml)|0.1 mL 100x Biotin conjugated SAA1 Detection Antibody|0.12 mL 100x Streptavidin-HRP|0.12 mL Detection Antibody Diluent|12 mL Assay Buffer|12 mL Sample Diluent|30 mL Wash Buffer Concentrate 20X|60 mL TMB Substrate|12 mL Stop Solution|12 mL Plate Sealer|3 pieces
Background	Serum amyloid A1 (SAA1) is a major acute-phase protein mainly produced by hepatocytes in response to infection, tissue injury and malignancy. When released into blood circulation, SAA1 is present as an apolipoprotein associated with high-density lipoprotein (HDL). SAA1 is a major precursor of amyloid A (AA), the deposit of which leads to inflammatory amyloidosis. Mature SAA1 consists of 104 amino acids with an apparent molecular weight of 12,500. Native SAA1 is a hexamer with each subunit assuming an antiparallel 4-helix bundle structure. Inflammatory cytokines such as IL-1β, IL-6 and TNF-α are major stimulants for hepatocyte expression of the SAA1 gene. SAA1 has been a clinical indicator and reliable biomarker for inflammatory diseases, chronic metabolic disorders and late-stage malignancy.
Gene Symbol	SAA1
Standard Curve	Serum Amyloid A (SAA1) ELISA Kit data image.

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