Human AKT1 ELISA Kit 1 x 96

Specifications

Product Data
Description	Human AKT1 ELISA Kit 1 x 96
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich
Assay Length	3.5 hours incubations; 0.5 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	78-5000pg/ml
Sample Type	Human serum, plasma, and other biological fluids.
Sample Volume	100µl
Specificity	This kit is used for quantitative detection of AKT1
Sensitivity	21pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	AKT1 Monoclonal Antibody Coated 96-well Plate in foil pouch with desiccant|1 plate Human AKT1 Standard (250ng/ml)|0.1 mL 100x Biotin conjugated AKT1 Detection Antibody|0.12 mL 100x SA-HRP Conjugate|0.12 mL Assay Buffer|40 mL Sample Diluent|20 mL Wash Buffer Concentrate 20X|60 mL TMB Substrate|12 mL Stop Solution|12 mL Plate Sealer|3 pieces
Background	AKT1 also known as RAC (Rho family)-alpha serine/threonine-protein kinase, is an enzyme that in humans is encoded by the AKT1 gene. AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) that belong to the AKT subfamily. AKT represents an important signaling hub, modulating many processes including metabolism, proliferation, cell survival, growth and angiogenesis by phosphorylating a range of downstream substrates in response to growth factor stimulation. In human cancers, AKT1 plays a central role in tumorigenesis. Recent studies using clinical specimens have shown that Akt protein is frequently activated in HER2/neu-overexpressing breast tumors. The overexpression of pAkt may be a powerful prognostic marker for predicting disease-free survival (DFS) and overall survival of breast cancer patients.
Gene Symbol	AKT1
Standard Curve	Data image of Human AKT1 ELISA Kit.

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