Human EGFR ELISA Kit 1 x 96

Specifications

Product Data
Description	Human EGFR ELISA Kit 1 x 96
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich
Assay Length	3.5 hours incubations; 0.5 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	78-5000pg/ml
Sample Type	Human serum, plasma and other biological fluids.
Sample Volume	100µl
Specificity	This kit is used for quantitative detection of EGFR
Sensitivity	54pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	EGFR Monoclonal Antibody Coated 96-well Plate in foil pouch with desiccant|1 plate Recombinant human EGFR Standard (250ng/ml)|0.1 mL 100x Biotin conjugated EGFR Detection Antibody|0.12 mL 100x SA-HRP Conjugate0.12 mLAssay Buffer|40 mL Sample Diluent|30 mL Wash Buffer Concentrate 20X|60 mL TMB Substrate|12 mL Stop Solution|12 mL Plate Sealer|3 pieces
Background	Epidermal growth factor receptor (EGFR) also known as Her1, ErbB1, or ErbB, is a type I transmembrane glycoprotein with an extracellular domain (ECD) that contains two cysteine-rich regions separated by a spacer. Mature human EGFR is a 170 kDa protein that is extensively and heterogeneously modified with N-linked glycosylation. EGFR is widely expressed on epithelial cells, predominantly in the gastrointestinal tract and breast, and is required for epithelial cell development and proliferation. EGFR binds the EGF family ligands EGF, Amphiregulin, TGF-α, Betacellulin, Epiregulin, HB-EGF, and Epigen. This results in activation of the kinase domains, tyrosine autophosphorylation, and internalization of the receptor-ligand complex. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. Alterations in the structure, expression, and signaling of EGFR are involved in the development and metastasis of a wide variety of cancers, particularly those of epithelial origin. Soluble receptors consisting of the extracellular domain of EGFR are generated by alternate splicing in humans and mice. The ECD can also be released by proteolytic cleavage. The soluble forms retain ligand binding capability and may dimerize with membrane bound EGFR, resulting in inhibition of its tyrosine kinase activity. Levels of the soluble EGFR are elevated in the serum of cervical and gastric carcinoma patients and in the urine of squamous cell carcinoma patients. Serum EGFR elevation is also associated with responsiveness to chemotherapy in advanced colorectal cancer. In contrast, serum EGFR is reduced in ovarian carcinoma, non-small cell lung cancer, and head and neck carcinoma. A decrease of serum EGFR relative to soluble ErbB2 is associated with decreased life expectancy in patients with metastatic breast cancer.
Gene Symbol	EGFR
Standard Curve	Human EGFR ELISA Kit .

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