Chicken LITAF ELISA KIT (96 T)

CAT#: EA800011

Chicken TNF-alpha ELISA Kit



CNY 3,200.00


货期*
3周

规格
    • 96 T

Product images

Specifications

Product Data
Description Chicken TNF-alpha ELISA Kit
Size 96 T
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 4 hours
Signal Colorimetric
Curve Range 17.18-1100 pg/ml
Sample Type Serum, plasma, Cell culture supernatant
Sample Volume 100 uL
Specificity This assay recognizes both natural and recombinant Chicken TNF-a
Sensitivity 8 pg/mL
Reactivity Chicken
Interference No significant interference observed with available related molecules.
Components
  • Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate
  • Standard - lyophilized,2200pg/ml upon reconstitution,2 vials
  • lyophilized, Biotin-Conjugated antibody,2 vials
  • Concentrated Streptavidin-HRP,1 vial
  • Standard /sample Diluent,1 bottle
  • Biotin-Conjugate antibody Diluent,1 bottle
  • Streptavidin-HRP Diluent,1 bottle
  • 20 x Wash Buffer Concentrate,1 bottle
  • Substrate Solution,1 bottle
  • Stop Solution,1 bottle
  • Plate Cover Seals,4 pieces
Background TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein . Membrane bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer . Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced primarily by macrophages, but it is produced also by a broad variety of cell types including lymphoid cells, mast cells, endothelial cells, cardiac myocytes, adipose tissue, fibroblasts, and neuronal tissue . Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, ERK (p44/42), p38 MAPK and NF-κB) promotes the survival of cells, while TNF-α mediated activation of caspase-8 leads to programmed cell death . TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis . TNF-α causes many of the clinical problems associated with autoimmune disorders such as rheumatoid arthritis, ankylosing spondylitis, inflammatory bowel disease, psoriasis, hidradenitis suppurativa and refractory asthma. The role of TNF-α in autoimmunity is underscored by blocking TNF-α action to treat rheumatoid arthritis and Crohn’s disease .
Gene Symbol LITAF
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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