Human PF4 ELISA KIT (96 T)

CAT#: EA800058

Human CXCL4 Immunoassay



CNY 3,200.00


货期*
3周

规格
    • 96 T

Product images

Specifications

Product Data
Description Human CXCL4 Immunoassay
Size 96 T
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 4 hours
Signal Colorimetric
Curve Range 15.62-1000pg/ml
Sample Type Serum, plasma, Cell culture supernatant
Sample Volume 100 uL
Specificity This assay recognizes both natural and recombinant human CXCL4.
Sensitivity 8 pg/mL
Reactivity Human
Interference No significant interference observed with available related molecules.
Components
  • Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate
  • Standard - lyophilized,1000 pg/ml upon reconstitution,2 vials
  • Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials
  • Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial
  • Standard /Sample Diluent - 16 ml/vial,1 bottle
  • Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle
  • Streptavidin-HRP Diluent - 16 ml/vial,1 bottle
  • Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle
  • Substrate Solution - 12 ml/vial,1 bottle
  • Stop Solution - 12 ml/vial,1 bottle
  • Plate Cover Seals,4 pieces
Background Platelet Factor 4 (PF4), also known as CXCL4, is an 8 kDa member of the CXC chemokine family that shares features with CXCL8/IL-8 and CXCL7/thymus chemokine-1/ NAP-2/β-thromboglobulin. Mature human PF4 shares 65-76% amino acid sequence identity with mouse, rat, bovine, ovine and porcine PF4. The active protein is a tetramer of PF4 subunits that forms a ring of heparin-binding positive charges from sites at the C-terminal region of each monomer. Megakaryocytes synthesize PF4 and store it in platelet α-granules. Secretion from activated platelets can produce micromolar levels in serum and over 100-fold higher within clots. In contrast to other CXC chemokines, PF4 does not contain an ELR motif and lacks binding to nearly all chemokine receptors. A potential high-affinity G-protein-coupled receptor for PF4, the CXCR3 isoform CXCR3B, is expressed in human but not mouse. In most cases, it is likely that cell surface binding and signaling properties of PF4 are due to binding of glycosaminoglycan chains, particularly chondroitin sulfates.
Gene Symbol PF4
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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