Mouse Egf ELISA KIT (96 T)

Specifications

Product Data
Description	Mouse EGF Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	3.9-250 pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant mouse EGF.
Sensitivity	2pg/mL
Reactivity	Mouse
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Epidermal growth factor (EGF) is a 6 kDa non-glycosylated monomeric protein consisting of 53 amino acid (aa) residues. It is derived from a 150-170 kDa type I transmembrane pro-protein. Mature and soluble pro-EGF can be detected in various body fluids and secretions including blood, urine, saliva, and milk. Within the active EGF domain, mouse EGF shares 70%, 73%, and 74% aa sequence identity with human, rat and porcine EGF, respectively. Cells known to express EGF include platelets, cerebral neurons, astrocytes, cerebellar Purkinje cells, cells of the Brunner (duodenum) and submandibular glands, non-pigmented ciliary epithelium, and cells of the anterior pituitary. A large number of diverse biological effects have been attributed to EGF. It is a mitogen that stimulates the proliferation of different types of cells, especially fibroblasts and epithelial cells. During development, EGF modulates growth and differentiation of thymocytes, in the passage from the double-negative to the double-positive (CD4+/CD8+).
Gene Symbol	Egf

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