Porcine IFNG ELISA KIT (96 T)

CAT#: EA800178

Porcine IFN-γ ELISA KIT



CNY 3,200.00


货期*
3周

规格
    • 96 T

Product images

Specifications

Product Data
Description Porcine IFN-γ ELISA KIT
Size 96 T
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 4 hours
Signal Colorimetric
Curve Range 25-1600 pg/ml
Sample Type Serum, plasma, Cell culture supernatant
Sample Volume 100 uL
Specificity This assay recognizes both natural and recombinant Porcine IFN-?
Sensitivity 12 pg/mL
Reactivity Porcine
Interference No significant interference observed with available related molecules.
Components
  • Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate
  • Standard - lyophilized,1600pg/ml upon reconstitution,2 vials
  • lyophilized, Biotin-Conjugated antibody,2 vials
  • Concentrated Streptavidin-HRP,1 vial
  • Standard /sample Diluent,1 bottle
  • Biotin-Conjugate antibody Diluent,1 bottle
  • Streptavidin-HRP Diluent,1 bottle
  • 20 x Wash Buffer Concentrate,1 bottle
  • Substrate Solution,1 bottle
  • Stop Solution,1 bottle
  • Plate Cover Seals,4 pieces
Background IFN-γ is a dimerized soluble cytokine that is the only member of the type II class of interferons. It plays key roles in both the innate and adaptive immune response against viral and intracellular bacterial infections and for tumor control. It alters transcription in up to 30 genes producing a variety of physiological and cellular responses. IFN-γ activates the cytotoxic activity of innate immune cells such as macrophages and NK cells. IFN-γ production by NK cells and antigen-presenting cells (APCs) promotes the cell mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increased class I and class II MHC expression, and enhancing peptide antigen presentation. The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes to form (IFNGR1/IFNGR2) 2 -IFN-γ dimer. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2 and Stat1. The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-γ or IFNGR knockout mice and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-γ also appears to have a role in atherosclerosis.
Gene Symbol IFNG
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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