Rat Timp1 ELISA KIT (96 T)

Specifications

Product Data
Description	Rat TIMP-1 ELISA KIT
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	31.25-2000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant rat TIMP-1
Sensitivity	15 pg/ml
Reactivity	Rat
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	The mammalian TIMP family includes four members that share structural similarity . All TIMP proteins have 12 conserved cysteine residues that form six intrachain disulfide bonds, resulting in an extremely stable protein with six loops. The TIMP protein has two structurally and functionally distinct domains: the N-terminal domain consisting of loops 1-3 that are responsible for tight, but non-covalent binding to the active MMPs in a 1:1 stoichiometry; and the C-terminal domain consisting of loops 4-6 that enhances the enzyme-inhibitor interactions. In the case of TIMP-1, the C-terminal domain has also been shown to bind the hemopexin-like domain of pro-MMP-9. TIMP-1 stimulates erythropoiesis, inhibits angiogenesis and is an antiapoptotic agent for B cells. These TIMP-1 functions may be independent of MMP inhibition . Rat TIMP-1 is a 28-35 kDa secreted glycoprotein . The protein is synthesized as a 217 amino acid (aa) precursor that contains a 23 aa signal peptide and a 194 aa mature form . Rat TIMP-1 shares 85%, 70%, 68%, and 66% aa sequence identity with its mouse, human, porcine, and canine counterparts, respectively . Among the three known rat TIMPs, TIMP-1 shares 40% and 35% aa identity with TIMP-2 and TIMP-3, respectively . TIMP-1 is widely expressed in many cells such as fibroblasts, osteoblasts, endothelial cells, granulosa cells, dendritic cells, vascular smooth muscle cells, adipocytes, and monocytes .
Gene Symbol	Timp1

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