PIMT (TGS1) (NM_024831) Human 3' UTR Clone

CAT#: SC209636

3' UTR clone of trimethylguanosine synthase homolog (S. cerevisiae) (TGS1) for miRNA target validation



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CNY 4,845.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (2)
Firefly luciferase assay kit, 150 assays
    • 1 kit

CNY 1,520.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

Specifications

Product Data
Product Name PIMT (TGS1) (NM_024831) Human 3' UTR Clone
Vector pMirTarget
Synonyms NCOA6IP; PIMT; PIPMT
ACCN NM_024831
Insert Size 1772 bp
Sequence Data
>SC209636 3’UTR clone of NM_024831
The sequence shown below is from the reference sequence of NM_024831. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
CTAATTCGAAGACCAGCCTCTGAAACCTAACTATGCAGCAGTGCGAGGACAAAAGATCATGGAGTGGTC
AAAATATTCAGATGAGACATTTGGCATGTCTTCCTTTATTCACTGATATTTTCTACCCATGGTCTTATA
TCACCGTATGAAATGGAAACTTACAGGACTTAAATATCAGTGAAATATTTTGAGATCTTTGAATAATTC
CTTTAGAGGAATTATACAAAATTAATATATATGAGTCCTTTGTAATTTATTTTTTTTTGAGACAGGATC
TCACTTTTACCGCCCAGGCTGGAGTGCAGTGCCATGATCACAGCTCACTGCAGGTTCAGCCTTCTGAGT
TCAAGCAATCCTTCTGTCTCAGTCTCCTCAGTAGCTGGGGCTTTAGGTGGGCACTGCCACACCGTACTA
ATTTTTGTATTTTTTGTAGAGACGAGGTCCCACCATGTTGCCCAGGCTGGTGTCAAACTCCTGGGCTCA
GTCAGTCCCCCCATCTCACCCTCCCCAAGTGCTGGAATTACAGGCGTGAGCTACTGTGCCCAGCCTTAC
GGACATCCTTTTGAATTATCTTTTTCACTCATAGAATATGAATACATTTATTTAGACTTTTTCTAGAAC
TTTCCTGTTTTCATGTCTTTGCTTCATCTGGAATTGGCTTAACACCCTTTTATAAAGTTTGTGTTTGTA
AAATTTCCATTGTGACATCAATACGCAATATATTTTGTAATATAGGAGTTTCTATTTTTTTATTAAAAT
GGCAATGAAAGCAAGAGGGATATGTGTTGCTTAATTATTCATCTAAAAAGTTTGTTCAGTCATTTTTAC
AAGTAGGCAAAAAAATAGTGACATACAACACTTGTCCATTGAAGGTTAGACCTGGGACTTCTATATTTT
AATAATATGAGCGTGTTAAACATTAAACAGAACATGATACTGGCTGGGATAAGATTAGAGAATTGAAAC
AAATTTGGGGAATTTCCAATAAATACTAGAAAATTAGACTAGAAAAATAGCTGTTATATAAATAACTTT
GTTGGAATATGCTAATTTTAGTGTCTTGAAAGTTTACAATTTATCTGATTGCTCTGCAGTACAGTAGCA
ACTGAGCTGATCAATAAAGGTGAAATTACTTTTTCATTAATTCGCAATTTCAAAATCTGTCCAAATGTT
CTTGGTTTTTAAACTTTATGTAGCATTCTTTTTTTTTTTTTTAGACAGAGTCTTGCTCTGTCGCCCAGG
CTGGAGTGCAATGGCGTGATCTCAGCTTACTGCAACCTCCACCTCCTACCTCAGCCTCCCGAGTAGCTG
GGACTACAGGCACCTGCCACCACATCCAGCTAATTTTTTATATTTTTAGTAGCTATGGGGTTTCACCGT
GTTAGCCAGGATGGTCTCGATCTCCTGACCTTGTGATCCAACCACCTCAGCCTCCCGTAGTTCTGGGAT
TACAGGCGTGAGCCACCGCGCCTGGCTTTATGTAGCATTCTTAAAGTCACTAGGGAGATGGCAGGTGAA
GTAAAGGCAGATTTCCAAAATCACTAATTTTTTTTAGTTTTTTGTCACTTAACCTTTCTTGCATATACT
TCTTTCCACAGCTCATTTTCTTACTTGTATATTAATAAAGCTAACTCATCTTCCTGAATATACATGATT
TTACATGAGAAGAGCAGGAAGCTTAGGCTTGGTTAGCTAAAACTAAGATATTGCTGCCCACTAATCACA
CAGGGATAAAACTAATAGACTGTTAAGATTGTCAGTACTCAATAGGA
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq NM_024831.8
Synonyms NCOA6IP; PIMT; PIPMT
Summary Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation.[UniProtKB/Swiss-Prot Function]
Locus ID 96764
MW 67.6
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