SFPQ (NM_005066) Human 3' UTR Clone

Specifications

Product Data
Product Name	SFPQ (NM_005066) Human 3' UTR Clone
Vector	pMirTarget
Synonyms	POMP100; PPP1R140; PSF
ACCN	NM_005066
Insert Size	1550 bp
Sequence Data	Insert Sequence (showhide) >SC210513 3’UTR clone of NM_005066 The sequence shown below is from the reference sequence of NM_005066. The complete sequence of this clone may contain minor differences, such as SNPs. Blue=Stop Codon Red=Cloning site GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC GAAGGCCCAAACAAAAAACCCCGATTTTAGATGTGATATTTAGGCTTTCATTCCAGTTTGTTTTGTTTT TTTGTTTAGATACCAATCTTTTAAATTCTTGCATTTTAGTAAGAAAGCTATCTTTTTATGGATGTTAGC AGTTTATTGACCTAATATTTGTAAATGGTCTGTTTGGGCAGGTAAAATTATGTAATGCAGTGTTTGGAA CAGGAGAATTTTTTTTTCCTTTTTATTTCTTTATTTTTTCTTTTTTACTGTATAATGTCCCTCAAGTTT ATGGCAGTGTACCTTGTGCCACTGAATTTCCAAAGTGTACCAATTTTTTTTTTTTTACTGTGCTTCAAA TAAATAGAAAAATAGTTATAATATTGATCTTCAACTTTGCCATTCATGCTTCTATGCATATTAGGCTAC GTATTCCACATTGAAAGCATGAGAGTGTCTAGGCCTTTGAATGGCATATGCCATTTCTGGGAAATGCAT CTGGAGGCTAAGTATTGCTTTCTACAAATAATTGCCCCCTTTGTTTTAAAAAGAAGAAATGCATATTGA AGTAGTTTGATGATTTGTTTGGCATATAGGAAGCACGCTGGTGCTAAGTATTTTTTAAATGGTTATGTA AGCAAAGCTGAACTGTAAATCTTCAGGAATATGTATTAAGATTGTGGAATGGGTGTAAGACAATTGGTA GGGGGTGAAAGTGGGTTTGATTAAATGGATCTTTTATGGCCCTATGATCTATCCTTTACTTGAAAGCTT TTGAAAAGTGGAAAGGTCATTTTGTTGCATTTCCCCATTTCTTGTTTTTAAAAGACCAACAAATCTCAA GCCCTATAAATGGCTTGTATTGAACTTTTACATTTGAATTAAAGATGTTAAACATGAAGTCTTTTCATG TATTCAAGTGATTTATAAAGATGGGGTGTAGTCTAAAAAACAAAACTTGAAAGTAAGAAGGTTTAGTAA CTGGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGCAAATCATGA GATCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAAATTA GCTGGGCGTGGTGGCAGGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGCCAGGAGAATTGCTTGAAC CTGGAGATGGAGGTTGCAGTGAGCTGAGACTGCCACTGCACTCCAGCCTGGACAACAGAGCGAGACTCT GTCTCAAAAAAAAAAAAAGGTTTAGTACTGAAGTAGGTTTAGTTATATATACATTTGTACTCTGTAACT TTTACATCTCCTTGTGCTACTTCTGTTGGGCAAAATCTATAGGACTATAAAAATCTATTGGTTCTCTTC TAAGAGCTAGTGGTGATTGTTAACAAATAGGGTTGCAGATAATCATTCTTATTGTAAATTTAACTTGTT TTCACATAGTTTCGTTTTCATGTAGCACTAAATCTGGAGAAAGTTGCTCATGCTACACAGTACAAATAT CCCTTCCTCCCACCATAAAGGCTTGGAATGTT ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites	SgfI-MluI
OTI Disclaimer	Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq	NM_005066.3
Synonyms	POMP100; PPP1R140; PSF
Summary	DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45, a phosphorylated form is sequestered by THRAP3 from the pre-mRNA in resting T-cells; T-cell activation and subsequent reduced phosphorylation is proposed to lead to release from THRAP3 allowing binding to pre-mRNA splicing regulatotry elements which represses exon inclusion. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer binds DNA (PubMed:25765647). The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Functions as transcriptional activator (PubMed:25765647). Transcriptional repression is mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation (By similarity). Required for the assembly of nuclear speckles (PubMed:25765647). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728).[UniProtKB/Swiss-Prot Function]
Locus ID	6421
MW	59.9

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