Pabpc1 (BC003870) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	PABP, PabpI
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC003870 CCGGCTCCGTCCCCCGGCCCCGCACTCGCTCTCCTCCTCTCACGGGAAAGGTCGCGGCCTGCGGCCCCGC GGGCAGCCGTGCCGAGATGAACCCCAGCGCCCCCAGCTACCCCATGGCCTCTCTGTACGTGGGGGACCTG CACCCCGACGTGACCGAGGCGATGCTCTACGAGAAGTTCAGCCCGGCCGGGCCCATCCTCTCCATCCGGG TCTGCAGGGACATGATCACCCGCCGCTCCTTGGGCTACGCGTACGTGAACTTCCAGCAGCCGGCGGACGC GGAACGTGCTTTGGACACCATGAATTTTGATGTTATAAAGGGCAAGCCAGTACGCATCATGTGGTCTCAG CGTGATCCATCACTTCGCAAAAGTGGAGTAGGCAACATATTCATTAAAAATTTGGACAAATCCATCGACA ATAAAGCACTATATGATACGTTTTCTGCGTTTGGTAACATCCTTTCATGTAAGGTGGTTTGTGATGAAAA TGGCTCCAAGGGCTATGGATTTGTACACTTTGAAACACAGGAAGCAGCTGAAAGAGCTATTGAAAAAATG AATGGGATGCTTCTAAATGATCGTAAAGTGTTTGTTGGACGATTTAAATCTCGGAAGGAACGAGAAGCAG AGCTTGGAGCCAGGGCAAAGGAGTTCACCAATGTTTACATCAAGAACTTCGGAGAAGACATGGATGATGA GCGCCTTAAGGAACTCTTTGGCAAGTTTGGGCCTGCCTTAAGTGTGAAAGTAATGACAGATGAAAGTGGA AAATCCAAAGGATTTGGATTTGTAAGCTTTGAAAGGCATGAAGATGCGCAGAAAGCTGTGGATGAGATGA ATGGGAAGGAGCTCAATGGAAAACAGATTTATGTTGGACGAGCTCAGAAAAAAGTGGAACGGCAGACGGA ACTTAAGCGCAAATTTGAGCAGATGAAGCAAGATAGGATCACCAGATATCAGGGTGTGAACCTTTATGTG AAAAATCTTGATGACGGGATTGATGATGAGCGTCTCCGGAAGGAGTTTTCTCCGTTTGGTACAATCACCA GTGCAAAAGTAATGATGGAGGGTGGGCGCAGCAAAGGGTTTGGTTTTGTATGTTTCTCATCCCCTGAAGA AGCCACTAAAGCAGTTACAGAGATGAATGGTAGAATTGTGGCCACGAAGCCACTGTATGTAGCTTTAGCT CAGCGCAAAGAAGAGCGCCAGGCTCACCTCACTAACCAGTATATGCAGAGGATGGCAAGTGTACGAGCTG TGCCCAACCCCGTGATCAACCCCTACCAGCCAGCACCTCCTTCAGGTTACTTCATGGCAGCTATCCCACA GACTCAGAACCGTGCTGCATACTATCCTCCTAGCCAAATTGCTCAACTAAGACCAAGTCCTCGCTGGACT GCTCAGGGTGCCAGACCTCATCCATTCCAGAATATGCCCGGTGCTATCCGCCCAGCTGCTCCTAGACCAC CATTTAGTACGATGAGACCAGCTTCCTCACAGGTTCCACGAGTCATGTCAACACAGCGTGTTGCTAACAC ATCAACACAGACAATGGGTCCACGTCCTGCAGCTGCTGCTGCTGCAGCCACTCCTGCTGTCCGCACCGTT CCCCAGTATAAATATGCTGCGGGAGTCCGCAATCCCCAGCAACATCTTAATGCACAGCCACAAGTTACCA TGCAACAGCCTGCTGTTCATGTGCAAGGTCAAGAACCTTTAACTGCTTCCATGTTGGCATCTGCGCCCCC GCAAGAGCAGAAGCAAATGTTGGGTGAACGGCTGTTTCCTCTTATCCAAGCCATGCACCCTTCTCTTGCT GGTAAAATCACTGGCATGCTGTTGGAGATTGATAACTCAGAATTACTTCACATGCTCGAGTCTCCAGAGT CTCTCCGCTCAAAGGTTGATGAAGCTGTAGCTGTACTACAAGCCCACCAAGCGAAAGAGGCTGCCCAGAA AGCAGTGAACAGTGCCACTGGTGTTCCAACTGTCTAAATTGATCAGGGACCACGAACAGAAACTCGTGCT TCACCGAAGAAAAATATCTTAAACATCGAAAAATTTAAATATTATGAAAAAAAACATTGCAAAATATAAA ATAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC003870
Insert Size	1911 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003870, AAH03870
RefSeq Size	2118 bp
RefSeq ORF	1911 bp
Locus ID	18458
Gene Summary	Binds the poly(A) tail of mRNA, including that of its own transcript. May be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed (By similarity). By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability (By similarity).[UniProtKB/Swiss-Prot Function]

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