Neu1 (BC004666) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA407268; AA407316; Aglp; Apl; Bat-7; Bat7; G9; Map-2; Neu; Neu-1
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC004666 AGTTCCTGGACAGGGATCGCCGGGAGCTATGGTGGGGGCAGACCCGACCAGACCCCGGGGACCGCTGAGC TATTGGGCGGGCCGTCGGGGTCAGGGGCTCGCAGCGATCTTCCTGCTCCTGGTGTCCGCGGCGGAATCCG AGGCCAGGGCAGAGGATGACTTCAGCCTGGTGCAGCCGCTGGTGACCATGGAGCAGCTGCTGTGGGTGAG CGGGAAGCAGATCGGCTCTGTAGACACTTTCCGCATCCCGCTCATCACAGCCACCCCTCGGGGCACGCTC CTGGCCTTCGCTGAGGCCAGGAAAAAATCTGCATCCGATGAGGGGGCCAAGTTCATCGCCATGAGGAGGT CCACGGACCAGGGTAGCACGTGGTCCTCTACAGCCTTCATCGTAGACGATGGGGAGGCCTCCGATGGCCT GAACCTGGGCGCTGTGGTGAACGATGTAGACACAGGGATAGTGTTCCTTATCTATACCCTCTGTGCTCAC AAGGTCAACTGCCAGGTGGCCTCTACCATGTTGGTTTGGAGTAAGGACGACGGCATTTCCTGGAGCCCAC CCCGGAATCTCTCTGTGGATATTGGCACAGAGATGTTTGCCCCTGGACCTGGCTCAGGCATTCAGAAACA GCGGGAGCCTGGGAAGGGCCGGCTCATTGTGTGTGGACACGGGACGCTGGAGCGAGATGGGGTCTTCTGT CTCCTCAGTGATGACCACGGTGCCTCCTGGCACTACGGCACTGGAGTGAGCGGCATTCCCTTTGGCCAGC CCAAACACGATCACGATTTCAACCCCGACGAGTGCCAGCCCTACGAGCTTCCAGATGGCTCGGTCATCAT CAACGCCCGGAACCAGAATAACTACCATTGCCGCTGCAGGATCGTCCTCCGCAGCTATGACGCCTGTGAC ACCCTCAGGCCCCGGGATGTGACCTTCGACCCTGAGCTCGTGGACCCTGTGGTAGCTGCAGGAGCACTAG CCACCAGCTCCGGCATTGTCTTCTTCTCCAATCCAGCCCACCCTGAGTTCCGAGTGAACCTGACCCTGCG CTGGAGTTTCAGCAATGGTACATCCTGGCTGAAGGAGAGGGTCCAGGTGTGGCCGGGACCCAGCGGCTAC TCGTCCCTGACAGCCCTGGAAAACAGCACGGATGGAAAGAAGCAGCCCCCGCAGCTGTTCGTTCTGTACG AGAAAGGCCTGAACCGGTACACCGAGAGCATCTCCATGGTCAAAATCAGCGTCTACGGCACGCTCTGAGC CCCGTGCCCAAAGGACACCAAGTCCTGGTCGCTGACTTCACAGCTCTCTGGACCATCTGCAGAGGGTGCC TGAAACACAGCTCTTCCTCTGAACTCTGACCTTTTGCAACTTCTCATCAACAGGGAAGTCTCTTCGTTAT GACTTAACACCCAGCTTCCTCTCGGGGCAGGAAGTCCCTCCGTCACCAAGAGCACTTTTTTCCAGTATGC TGGGGATGGCCCCTGTCCATTCTCTTCCAGGACAACGGAGCTGTGCCTTTCTGGGACAGGATGGGGGAGG GGCTCCCCCTGGAGAGATGAACAGATACGAACTCAGGGAACTGAGAAGGCCCGGTGTCCTAGGGTACAAA GGCAGGTACTAGATGTGATTGCTGAAAGTCCCCAGGGCAGAGTGTCCTTTCAGAGCAAGGATAAGCACAC CTACGTGTGCACCTTTGATTATTTATGAATCGAAATATTTGTAACTTAAAATTTTTGATGCAGAAAAAAA AAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC004666
Insert Size	1230 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC004666, AAH04666
RefSeq Size	1758 bp
RefSeq ORF	1230 bp
Locus ID	18010
Gene Summary	Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.[UniProtKB/Swiss-Prot Function]

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