Dohh (NM_133964) Mouse Untagged Clone
CAT#: MC200225
Dohh (untagged) - Mouse deoxyhypusine hydroxylase/monooxygenase (Dohh), (10ug)
CNY 2,400.00
Product images
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Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 1110033C18Rik; AI585913; AL022869; Hlrc1 |
Vector | PCMV6-Kan/Neo |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>BC002295 sequence for NM_133964
GCAGCGACTCTCTCCATCTGTCCCGAGTATACCGAGCTTATTGACGAGTCCCTGTAGACGAAAACACGGT TTCTACTGCCTAGAATCCGGCCTTGCAGATCACGTGCTGGGGAGGCCGTTGCCATGGTAACGGAGCAAGA GATAGAGGCCATAGGGAAGACCTTGGTGGACCCCAAGCAGCCCCTGCAGGCCCGCTTCCGGGCCCTATTT ACACTTCGGGGGCTTGGTGGCCCTGATGCTATCTCGTGGATCAGCCGGGGCTTTGAGGACAGCTCTGCTC TTCTGAAGCACGAGCTGGCCTACTGCCTGGGCCAGATGAGGGACGTACGGGCCATCCCTGTGCTGGTGGG TGTGCTGCAGGACACGAGCCAGGAGCCCATGGTGCGTCACGAGGCAGGGGAAGCCCTGGGGGCGATTGGT AACCCGGAAGTTCTAGGCCTCCTGAAGCAGTATTCTACGGACCCAGTGGTTGAGGTGGCTGAGACGTGCC AGCTCGCCGTTGGTCGTCTAGAGTGGCTACAGCAGCACCCGGGGGAGGCCACATGTGCAGGACCCTACCT CTCAGTGGACCCAGCACCCCCTGCAGCAGAGCAGGATGTGGGGAGGCTGCGGGAGGCCCTGCTGGATGAG GCACGGCCACTCTTTGAGCGATACCGTGCCATGTTTGCCCTGCGCAATGTGGGTGGCAAGGAGGCTGCCT TGGCCCTGGCTGAAGGCCTGCAGTGTGGCAGTGCGCTCTTCCGCCACGAGGTGGGCTATGTGCTGGGTCA GCTGCAGCACGAGGCAGCTGTGCCCGGGCTGGCAGCCACATTGGCGCGGACCACTGAGAGTCCCATGGTG CGGCACGAGTGCGCAGAGGCCCTGGGTGCCATCGCCAGACCTGCCTGCCTGGCTGCATTGCGAGAGCACA TTGAGGACCCTGAGCAGGTGGTGCGGGAGAGCTGCGAGGTGGCCCTGGACATGTATGAGTATGAGAGCAG CCAGGATTTCCAGTATGCAGACGGGCTGGAGCGCCTGCGGCCACCACCCTAACCTAGCGCCTGGCTGCCT GCCCTGGGGCTGCCTCCTCTGCTGCTGCCCTGGCCCCGGCCATGTGTGCTGTGCCTCACATCCAGGTTTA GGGAGCAGAGCAGCTGAGGGGCCCTGTGTCCCACTCGCCTCACCTCCTTGCTGGGTGCTGTCTGCTCTGT GGTTCTGTGTCTCCCATGCCCTCCACCAGCAGAGGTGGCAGTGCAGACGCTTGCAGGGTTGGGAAAGTTG TGGGGGCAAGGGTGTGTGGGGTACACCTCCCATCTGGGTCAAGCAGGGAGTACAAGCCAGCCCCAGCGTG CTGCATGAATAAAATAAGTTATTTTTCACAAAAAAAAAAAAAAAAAA |
Restriction Sites | RsrII-NotI |
ACCN | NM_133964 |
Insert Size | 909 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC002295, AAH02295 |
RefSeq Size | 1377 bp |
RefSeq ORF | 909 bp |
Locus ID | 102115 |
UniProt ID | Q99LN9 |
Gene Summary | Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG204226 | Dohh (tGFP-tagged) - Mouse deoxyhypusine hydroxylase/monooxygenase (Dohh) |
CNY 2,850.00 |
|
MR204226 | Dohh (Myc-DDK-tagged) - Mouse deoxyhypusine hydroxylase/monooxygenase (Dohh) |
CNY 2,400.00 |
|
MR204226L3 | Lenti ORF clone of Dohh (Myc-DDK-tagged) - Mouse deoxyhypusine hydroxylase/monooxygenase (Dohh) |
CNY 4,750.00 |
|
MR204226L4 | Lenti ORF clone of Dohh (mGFP-tagged) - Mouse deoxyhypusine hydroxylase/monooxygenase (Dohh) |
CNY 4,750.00 |