Wdr77 (BC005755) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610003I18Rik; 2610312E17Rik; C79984; p44/MEP50
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC005755 CTCGTCGAGTCTGGGTCTAGGTTAGATTTGGAATCGTGGAGATGCGGAAGGACACCCCTCCTCCGCTCGT GCCCCCGGCGGCCCGCGAGTGGAACCTGCCCCCCAATGCGCCCGCATGCATGGAACGTCAATTGGAGGCT GCACGGTACCGGTCTGATGGTTCCCTTCTGCTCGGGGTCTCCAGCCTGAGTGGTCGCTGCTGGGTAGGTT CTCTGTGGTTTTTCAAGGATCCTAGTGCGGCCCCCAACGAAGGTTTCTGCTCTGCTGGCGTCCAGACCGA GGCTGGAGTAGCTGACCTCACTTGGGTGGGGGACAAAGGTATCCTAGTGGCTTCTGATTCAGGTGCTGTT GAATTGTGGGAGCTAGATGAGAACGAGACACTTATAGTCAGCAAGTTCTGCAAGTATGAGCATGATGACA TTGTGTCTACTGTCACTGTCCTGAGCTCTGGCACACAAGCTGTCAGTGGTAGCAAAGACTGCTGCATCAA AATTTGGGACCTGGCTCAGCAGGTATCACTGAATTCATACCGAGCTCACGCTGGACAGGTTACCTGTGTT GCTGCCTCTCCCCACAAAGACTCTGTGTTTCTTTCATGTAGTGAGGACAGTAGAATTTTGCTCTGGGATA CCCGCTGTCCCAAGCCGGCATCACAGATGGCCTGCAATGCCTCTGGCTACCTCCCTACCGCTTTGGCTTG GCATCCTCAGCAGAGTGAAGTCTTTGTTTTTGGTGACGAGAATGGATCTGTCTCCCTTGTGGACACCAAG AATGCAAGCTGTACCCTCAGCTCAGCTGTGCACTCCCAGGGTGTCACTAGACTGGTATTCTCCCCACACA GTGTCCCCCTCCTGACTTCTCTCAGTGAAGACTGTTCACTTGCTGTGCTGGATTCAAGCCTTTCTGAGGT GTTTAGAAGTCGAGCCCACAGAGACTTTGTGAGAGATGCTACGTGGTCTCCACTCAATCACTCCCTTCTT ACCACAGTTGGCTGGGACCATCAGGTCATCCACCATGTTGTGCCCTTAGAGCCTCTCCCAAACCCTGGAC CTGACAGTGTTGTGGAGTAGAATGGATTTCAGAAAAACCAAAACAAGCCCTCCGTCTGTAAGCGACTACT CGATTGCCCCTGCCTTCCATGTGTGAGAGCACAGGAGCCTTGTAGAGCATGTTTCCTCCCTAGCCCCGTG CAGTAACAGGCAGATTCTCAGCCTGAGGGAGGCTGCATCCCATAGTGACTCAGAGGAAGAAACTTCCTCT GTAAATGGATGTATGTGAGTACACGTGTGAGTGCGGTATATAGTTTGGAGTGGAGAAAATTATTCTTCAG CTTTCCCAAAGCAATGCTCTTTACCCCTGACAAAGTGACCAGAGGATTTTAATGCTTCCCATGTCTGGGA ATTGGCGATGTTAGGGATATGGAATGTGGGTATCCCTAGATTTTTGGGAATACTTCATACTACTCAGAGG TGCGTAACTTATTTTTATATAGAGTTTAATTCACTATTATGGGAATTACTTCCATATACAAAAGTCTAGC CCACTGTACCTTGAGAAGACAAAACAGTTTTGTACAAGTCCCTGTTATACTGAGTCAAATCCATTTTCTG GGTGAATAAATTTGGCCCTGCAGTGTAGCTCTTGTTCACCCTTTCCTTCCTTGGTGTTGTGTGTGAGAGA TGAGTGTTTTCCTGTCCTAGATTTCACAGATAAGTTGTAGGGATGATGGACCACTTAAAAGTGTTTCAGT GAAGTGGGAAAATTGACATGGATTGTTACAGAGAAATCTGTCATTTTTCCCACCTAAAAATTGATCTGGG TACCTTCCTCTGGAGAGCTGAAGTCTGAATGTATTTTTGGACTCCTAAGATTTGAGATCCAGCATCAGGG AAACTGTCGGAAATACTAAAAGGTGAAATAAAGACTTTTATCCTTGAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC005755
Insert Size	1029 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC005755, AAH05755
RefSeq Size	1958 bp
RefSeq ORF	1029 bp
Locus ID	70465
Gene Summary	Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (PubMed:19584108).[UniProtKB/Swiss-Prot Function]

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