Il33 (BC003847) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	NF-HEV, Il-33, Il1f11
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC003847 GAGAAATCACGGCAGAATCATCGAGAAACCTGAAAAATGAGACCTAGAATGAAGTATTCCAACTCCAAGA TTTCCCCGGCAAAGTTCAGCAGCACCGCAGGCGAAGCCCTGGTCCCGCCTTGCAAAATAAGAAGATCCCA ACAGAAGACCAAAGAATTCTGCCATGTCTACTGCATGAGACTCCGTTCTGGCCTCACCATAAGAAAGGAG ACTAGTTATTTTAGGAAAGAACCCACGAAAAGATATTCACTAAAATCGGGTACCAAGCATGAAGAGAACT TCTCTGCCTATCCACGGGATTCTAGGAAGAGATCCTTGCTTGGCAGTATCCAAGCATTTGCTGCGTCTGT TGACACATTGAGCATCCAAGGAACTTCACTTTTAACACAGTCTCCTGCCTCCCTGAGTACATACAATGAC CAATCTGTTAGTTTTGTTTTGGAGAATGGATGTTATGTGATCAATGTTGACGACTCTGGAAAAGACCAAG AGCAAGACCAGGTGCTACTACGCTACTATGAGTCTCCCTGTCCTGCAAGTCAATCAGGCGACGGTGTGGA TGGGAAGAAGGTGATGGTGAACATGAGTCCCATCAAAGACACAGACATCTGGCTGCATGCCAACGACAAG GACTACTCCGTGGAGCTTCAAAGGGGTGACGTCTCGCCTCCGGAACAGGCCTTCTTCGTCCTTCACAAAA AGTCCTCGGACTTTGTTTCATTTGAATGCAAGAATCTTCCTGGCACTTACATAGGAGTAAAAGATAACCA GCTGGCTCTAGTGGAGGAGAAAGATGAGAGCTGCAACAATATTATGTTTAAGCTCTCGAAAATCTAATGC AGTAAAACGCCTGTGCGTTCTGGGTTGAATGACTTAATGCTTCCAACTGAAGAAAGGGTAACAGAGAGAA AGAAAGCCATTCTTGGCTTACGATGTTGTGGAATGTTATTATGTAGAAAACTTCTTTTATTTCCTTTTCT TCAGCTACATGTTCAATAGACTCACAGATATATGACTTACGGCGTTGGTAAAGAAACTGAAGGAGATTCA GCCTTGCTCTTTCCTTTTCTCTGCCTTGAGTCCTGTATGAAATCACACTCACGGACTTCAGAAGAGCAGG CACCACAGTGCATGGTTTGCTTTAGAGAGGGTCCATTTCAAAAACCTTCATAAAAACAATGCAAAACAAG AAAACAACCGAACAAAAAAACCACCTATTTCCTGGTTCTAAACAAATGATTGTAATACTAGAGCAGTTAG TGGGAGGACCAGCTAGGGGGAGGATCACCTAGGGGAGGACCAGCTAGGGGGAGGACCAGCTGCTGCAAAG ACTGACTGTTTCTCACTTATAATAAAATGCCAAATGCCTCCGCAGATGCCCCAGGCAACCCTCAGATCAG CCCTTTCTGTGAAGAGTGGCGTTACCTGTGCTTGTTTCCTTCTTAAACTTCCAATTTTTCTCTTTTAACA CATTTAACATTTAACTTTAAGCAAGCCAGCTTACATTAGGAAGTGAAAGACATTTTAGTTCCCACCCGTG ATTGAAATCATTGACTATATCTAACAAGCTTAAAGTCTCCTGTAAGAACTGATCAGGATATACACTAGGA CATGCCAATAGAATGGGATCTCATGGTGCAGTCTGAAGCCCTCCAACTGGAGAGACGCTAACATCATCCT TCTCGCTGATTTCCAAGGAGCTATGACTTTGGATGCATGCATCTGCTTGGATGAGATGTCTCGGCTGCTT GCTTTCCTTATGCACACGTTCTGTTCAGCTTCACAGCAGCAATGCTCACGGTGGAATAGCTTAGCTTAGC TTCTGCCCCTTCTTTGGTTCTTTTGACCACCATATCCGTAACGGCTCTCCTACTCCCTCAGCTTTCTTTC TCTTTGCTCTGACGTCTATATGCCAACACACTTATTCCACTGTCTTTACCCTGCACTGCAGAATTTTACA TCTACCTACTGGTTACCAGGTTGTCCCCTGAACAACCTTCCTTTGTGTTTTACTGTTATTAAAGTAGTAA TATTTGTATTCAACCATGTAGTAATATTTTAAGCCACTAAAGGAATAGTTTTACTTATTTAGACAACAGC AATTTCTACTACATTTTTATAAGCTTAAAACTTACATGTTTTAAAACTTAAAACGATAAAGACAATAACA ACATTGATGGAGTATGATATGACAGTTCAGAAAGGGTTAGCTCTTATCTTCCAGTCGAGGAAACCTATTG TATACAATAGCTGGAGGAATGTATGATCAAAGAGGCCGGGAACCGCCGTGTAGGATCGTACGGCTGTAAC AGGTATAATTGTTTCATTAATTTGTCACAGTCTTACTGTAGAGGAATGTAAAGGCGGAATCTGCGTCATT CCTCTGGAAACCACAGTGTTGACTCTGTGAATCTGTACGATATCTTTAAAGTAGTAACTACGTAGTCAAA TGTGTTCTTGACGTTGTTCATAACTTTGAATAAACCATTTTTCAAAACCACGTGTGACCACAAAAAAAAA AAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC003847
Insert Size	801 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003847, AAH03847
RefSeq Size	2526 bp
RefSeq ORF	801 bp
Locus ID	77125
Gene Summary	Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.[UniProtKB/Swiss-Prot Function]

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