Slc29a1 (BC004828) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1200014D21Rik; AA407560; ENT1
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC004828 CTTATCTCCCAGCTACAGGGTGGCCCTTTATCTCTCCCAGCTTCGCAGGACCCAACCTCCTGCCTGAGCC TGAGCCAGGACCCCTTCCTGGGGAACGCCCAGGTACCTTTGGCTCTCACCGTCCTGCCAACATCGAGTCT ATAAATTTGCAAGAGCCAGAGGGAGGGAGCTGCCAGCCAGGCACAGCCGAGAACACTGGAGCCATGACAA CCAGTCACCAGCCTCAGGACAGGTATAAGGCAGTATGGCTTATCTTCTTTGTGCTGGGCCTGGGGACACT GCTCCCCTGGAATTTTTTTATGACCGCAACCAAGTATTTCACAAACCGCCTGGACGTGTCCCAGAATGTG TCCTCGGACACTGATCAATCATGCGAAAGCACCAAGGCCTTGGCTGACCCCACAGTGGCCTTGCCAGCCC GGAGTTCTCTCAGTGCCATCTTCAACAATGTCATGACCCTGTGTGCCATGCTGCCCTTGCTGGTCTTCAC CTGCCTCAACTCGTTTCTGCATCAGCGGATCTCTCAATCTGTTCGGATCTTGGGCAGCCTGCTGGCAATC CTGCTGGTATTCCTTGTCACTGCCGCCCTGGTGAAGGTGGAGATGGATGCTCTGACCTTCTTTGTCATCA CCATGATCAAGATTGTGCTCATCAATTCATTTGGTGCCATTTTGCAAGCCAGCCTTTTTGGTCTGGCAGG TGTCCTGCCAGCCAACTACACAGCCCCCATCATGAGTGGCCAGGGCCTGGCTGGCTTCTTCACCTCTGTC GCCATGATCTGTGCCATTGCCAGTGGTTCTGAGCTGTCAGAAAGCGCCTTTGGCTACTTCATCACAGCCT GTGCAGTTGTCATTTTGGCCATCCTGTGCTACCTGGCTCTGCCTCGGACGGAATTCTATCGCCATTACCT GCAGCTCAACCTTGCGGGGCCTGCAGAGCAGGAGACCAAGTTGGATCTCATAAGTAAAGGAGAGGAGCCA AAAGGAAGAAGAGAGGAATCTGGGGTGCCAGGCCCCAACTCTCCACCCACCAACAGAAACCAGTCTATCA AAGCCATACTTAAGAGTATCTGTGTCCCGGCTCTGTCTGTCTGCTTCATCTTCACGGTTACCATTGGGTT GTTCCCTGCTGTGACTGCTGAGGTGGAATCCAGCATCGCAGGCACAAGTCCCTGGAAAAGCTACTTCATT CCCGTGGCCTGTTTCTTGAATTTCAATGTCTTTGACTGGCTAGGCCGGAGCCTCACTGCTGTCTGCATGT GGCCTGGCCAGGATAGCCGCTGGCTGCCGGTTTTGGTCGCCTCGAGGATTGTGTTTGTTCCCCTGCTGAT GCTCTGCAACGTGAAGGCTCGCCACTGCGGCGCGCAGCGGCACCACTTCGTCTTTAAGCATGACGCCTGG TTCATCGCCTTCATGGCTGCCTTTGCCTTCTCCAATGGCTACCTCGCCAGCCTCTGCATGTGCTTCGGGC CCAAGAAAGTCAAACCAGCTGAGGCGGAGACAGCAGGAAACATCATGTCCTTCTTTCTGTGTCTGGGCCT GGCTCTGGGAGCTGTGTTGTCCTTCTTGTTAAGGGCACTTGTGTGACCCTGTGGGGACAGAAGAACTACA CTGCCTGCTTCCTGCTCACTTCCTTCCCTGCCAGGGACGAGCAGGGGTCGAGAGGGGCTGTTCTTCTAGC TGACTTCTGCTTTCCTCTGGACTGTGCTTCGCCCAGCTGTCCAGGAGCCAGCGATGGCCTGCGGGTGGAC TTGGAATTCAGGGTCAGAATGGCAAGGGCTCAATGGCCTCTGACTGACAGCTCCGACTGATGCCCGCTTA CTCCAAGCACAAGAGACTCCAGGGCCAAGAGAGATCTGTCCGCCTGCCTATCACAGGATAGGGCGGAGGC GGATGGCTGATTGGTGTCGTGTGACCTGATGTCCCTCCCCTTGCCCTTCTTCCTTCTGTGCCTGTTCCAT GTCCCCAGCCCTTGTCATTTTACTGCCTTTTTTATACTGACAGAAACCAGGTGCCTTCAGAGGCCATCTG ATTAAATAAACATTTTTTTTCTCCAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC004828
Insert Size	1383 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC004828, AAH04828
RefSeq Size	2071 bp
RefSeq ORF	1383 bp
Locus ID	63959
Gene Summary	Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs).[UniProtKB/Swiss-Prot Function]

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