Eif2s1 (BC005463) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	eIF2alpha, 35kDa
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC005463 GGCGTCCGGGGTTGGTGTTCCGAGTCTCCTCGTTGCCACTAAGCAGCACGGCGTGGGACCCTACTTCGGG ATTCACACATCCACTTCAGAATGCCGGGGCTAAGTTGTAGATTTTATCAACACAAATTTCCTGAGGTGGA AGATGTAGTGATGGTGAATGTAAGATCCATTGCTGAAATGGGGGCCTATGTCAGCTTGTTGGAATATAAT AACATTGAAGGCATGATTCTTCTTAGTGAATTATCCAGACGACGTATCCGTTCTATAAACAAACTGATCC GAATTGGCAGAAATGAATGTGTTGTTGTCATTAGAGTGGATAAAGAAAAAGGATATATAGATTTGTCAAA AAGAAGAGTTTCTCCAGAGGAAGCAATCAAATGTGAGGACAAATTCACAAAATCCAAAACTGTTTATAGC ATTCTTCGCCATGTTGCTGAGGTATTAGAATATACCAAGGATGAGCAGCTGGAGAGCCTGTTCCAGAGGA CTGCCTGGGTCTTCGATGACAAGTACAAGAGACCTGGATACGGTGCCTATGATGCTTTTAAGCATGCAGT CTCAGACCCATCTATCTTGGATAGTTTAGATTTGAATGAAGATGAAAGAGAAGTACTCATTAATAATATC AATAGGCGTTTGACCCCACAAGCGGTCAAAATTCGAGCAGATATTGAAGTAGCTTGCTATGGTTATGAAG GCATTGATGCTGTAAAAGAAGCCCTGAGGGCAGGTTTGAATTGTTCTACAGAAACCATGCCCATCAAGAT TAATCTAATAGCTCCACCCAGGTATGTGATGACAACAACGACCCTGGAGAGGACAGAAGGCCTGTCTGTC CTCAATCAGGCTATGGCAGTTATCAAAGAGAAGATCGAGGAGAAGAGGGGCGTCTTCAATGTTCAGATGG AGCCCAAAGTGGTCACAGATACAGATGAGACTGAACTTGCAAGGCAGCTGGAACGGCTGGAGAGAGAAAA TGCAGAAGTGGATGGAGATGATGATGCAGAAGAAATGGAAGCCAAAGCTGAAGATTAACGTTTTGGCAAA CAGTCCAATTTAAGGAGTACGAAGCAGCCCTTCCTGGCTGTCAACCCTAGACTTGAGAGTTTTCCAGTAT TGAAAACTTCAAAGCTGAATATTTTTATTTCCAAGTATTTAAGTATTCAACAAGCCAGAATCTAAATGCC CTCCTTCATGTCAGCTGTTTTCACATAGTGGCTCTAACACCTCAAGCATTTTTCAAGGGAGTGGCTTGAT TTGACCAGAGACAAATCTTAAACAGCAGTCCTAATATTGGGCCTACAGTTTCCATTTCTCATGTCTCTGG AATGGCACCCTTATGGTTCAGAGATTTACCAGGGACTCCAAACACAAACAATCCCAATCTTTCTATATAA AATGTATTCAAGCAAACATCAAATAAATTTCTGGGATATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC005463
Insert Size	948 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC005463, AAH05463
RefSeq Size	1479 bp
RefSeq ORF	948 bp
Locus ID	13665
Gene Summary	Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).[UniProtKB/Swiss-Prot Function]

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