Bgn (BC005452) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	BG, PGI, DSPG1, PG-S1, SLRR1A
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC005452 CCCACGCGTCCGCAGGAGCGAGCAGCTGCTCCCGGTTGGCCCTGACGGACAGACAAACCGACAGCCTGAC AACCTAGTCCACCAACTAAGCAGCCTGCACCTGGCTGCTTGTCCCTCCCCAGGAACATTGACCATGTGTC CCCTGTGGCTACTCACCTTGCTGCTGGCCCTGAGCCAGGCCTTGCCCTTTGAGCAGAAGGGTTTCTGGGA CTTCACCTTGGATGATGGGCTGCTCATGATGAATGATGAGGAGGCTTCAGGTTCAGACACCACTTCAGGT GTCCCCGACCTGGACTCTGTCACACCTACCTTCAGTGCCATGTGTCCTTTCGGTTGCCACTGCCACCTGC GGGTTGTTCAGTGCTCTGACTTGGGTCTGAAGACTGTGCCCAAGGAGATCTCACCTGACACCACACTGCT AGACCTGCAGAACAATGACATTTCTGAGCTTCGCAAGGATGACTTCAAAGGCCTCCAGCACCTCTACGCC CTGGTCTTGGTAAACAATAAGATCTCCAAGATCCATGAGAAGGCCTTTAGCCCTCTGCGGAAGCTGCAAA AACTCTACATCTCCAAGAACCACCTGGTGGAGATTCCTCCCAACCTGCCCAGCTCCCTGGTAGAACTACG AATCCATGACAACCGTATCCGCAAAGTGCCCAAGGGCGTGTTCAGCGGGCTCCGGAACATGAACTGCATT GAGATGGGCGGGAATCCCCTGGAGAACAGTGGCTTTGAACCAGGAGCCTTTGATGGCCTGAAGCTCAATT ACCTGCGCATCTCAGAGGCCAAGCTCACTGGCATCCCCAAAGATCTCCCTGAGACCCTGAACGAACTTCA CCTGGACCACAACAAAATCCAGGCTATTGAGTTGGAGGACCTACTTCGATACTCCAAGCTGTACAGGTTG GGCTTAGGTCACAATCAGATTCGGATGATTGAGAATGGGAGCCTGAGTTTTCTGCCTACCCTGAGGGAAC TTCACTTGGACAACAACAAGCTGTCCCGGGTGCCTGCTGGCCTCCCAGATCTCAAGCTCCTCCAGGTTGT CTATCTGCACTCCAACAACATCACCAAGGTGGGCATCAATGACTTCTGTCCTATGGGCTTCGGAGTCAAG AGGGCCTACTATAATGGCATCAGCCTCTTCAACAACCCTGTGCCCTACTGGGAAGTGCAGCCTGCCACCT TCCGCTGCGTTACTGACCGCCTGGCCATCCAATTTGGAAATTATAAGAAGTAGAGGCAGTGGTTGCCACC ATGGTGGCCTTGGTGAGAGTCTCTGAGGAACATAGCCAGATGAAGAAGCAACACCTTTGTTCCCCAATAT TAACTCACTGCCCCACCACAGCTTCCCCCTGACTCCTAAGCATGCATATATGCACATGGCCTGGCCCTCT CACCCATTCCCCTCAACCTTTGAAATTTAACATTCATCAACCATGTCCACTCAGAGACTCCCTATAAATC TTTCTTCTTGCTCATCCTGAAACTCAGATGTTTTTGGCAAGAGGGGCTAGGAAAGATGGATAGAGCACAC TGCCACCGCCATTGTTCCATCCAGGCATGTGTTCCTCCTCTTCCTTGCTCATGTCTGACTTCCAGCTCTC CTGGGCTCTGCTTGCTGCCCTTATCCTCTGGTGTTCTCTCTTCAACAAGTTCACTACCTGTCAATCCCAG CTACAACCTGGCTGTACTAACTCCTGGATCTTTCCCTCTCTCCAACCCTGTTATGCTTCCTGACACTTTT CTTCCTTCTGGAGTTATTGACCTGTCCCCTTCCATCTCTGGACCTAGGTCATATTTCTCCATCTTTGTCT CTTTCTGTATCTCCTTGCCTATATCTCTGTCTGTCTCTATTTCTGTCTCTCTGTCTCTGTGTATCTCTCT ATCTTTGTATCTGTCTCTCTCTGACAACACACACACACACACACACACACACACACACACACACACGGAT CATCTGCCCCAGGCTGCTTTCTGCTTCACAGGTCTCTAGCCAGTCCCTCCACAAACAAATATGGGGCAAC TATCTTCCTGATTGCCCTACCCAGAACTTGACCCCCCAACCCTGGAGGAAGCTGGAAGGTGGAGGCCCAG AATCCTGTCCATTTTGTCCAGGAAAGGGTTCATACTCTGCTATCAAGACGAGGATCAAGGAGCTTCCTAG CCCCTGGAGAGGCTCAGCAGGCCATCAGAGCCGCCAGAACCAGTTTGCATTGGCCCCTGCTCTCTCCCCA AGATGGCTAGGTCCCCTCCCTCACCCCTGGGTCCCTGATGTGGTAGGAGGTGATGGTCAGTTGCACCCAG CAAGAGGGAGTGCTGCTTATGAGGTCAGTTGTCTCTCAATTAAAGAAACACTGTGCAAAAAAAAAAAAAA AAA
Restriction Sites	RsrII-NotI
ACCN	BC005452
Insert Size	1110 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC005452, AAH05452
RefSeq Size	2383 bp
RefSeq ORF	1110 bp
Locus ID	12111
Gene Summary	This gene encodes a small, leucine-rich repeat proteoglycan that plays important roles in bone mineralization and connective tissue metabolism. The encoded preproprotein undergoes post-translational processing during which chondroitin sulfate or dermatan sulfate chains are attached before incorporation into the extracellular matrix. Mice lacking the encoded protein exhibit reduced growth rate and acquire diminished bone mass progressively with age. [provided by RefSeq, Oct 2015]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.