Rab27a (BC008173) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4933437C11Rik, 2210402C08Rik
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC008173 GCCAAGCACCCAAAAAAAGGCCAGTCGCACGGGGTCCCGAGCTCCATTTGGAAGGGAGTACCTAAGGGAT AGAGCACAGCGAGGACGCGGCACGTTGGGAATCTAGCACTGCAGGGACGCAACACCGCAGGGACAGCACC GGACAGAGCACCAAACCGGATTGAGTTTATCTCATCAACCCTGGCTGAGCTCCCTACAGCTATTTAGAGC CTGAAAAGAAAAAAAGTCCAAAGTGCTAAGCTAAAACTGAGAGCTTCAAACAGCTTCCAGCTAAGGACGC TACGCTGCACACTTCCGTGGCCATTGAGGTGAGGCACAGAGTCCTCCATCATGTCGGATGGAGATTACGA TTACCTCATCAAGTTCTTGGCCTTGGGAGACTCTGGGGTAGGGAAGACCAGTGTACTCTACCAGTACACT GATGGCAAGTTCAACTCCAAATTCATCACCACAGTGGGCATTGATTTCAGGGAAAAGAGAGTGGTGTACA GAGCCAATGGGCCAGATGGAGCTGTGGGCCGAGGCCAGAGAATCCACCTGCAGTTATGGGACACGGCGGG GCAGGAGAGGTTTCGGAGCTTAACCACTGCATTCTTCAGGGACGCTATGGGTTTCCTGCTTCTGTTCGAC CTGACAAATGAGCAAAGTTTCCTCAATGTCCGAAACTGGATAAGCCAGCTACAGATGCACGCGTACTGTG AAAACCCAGATATAGTGCTGTGTGGAAATAAGAGTGACCTGGAAGACCAGAGGGCAGTGAAAGAGGAGGA GGCCCGGGAACTTGCCGAGAAGTACGGAATCCCCTATTTTGAAACCAGTGCTGCCAACGGGACAAACATA AGCCACGCGATTGAGATGCTCCTGGACCTGATCATGAAGCGGATGGAGCGGTGTGTGGACAAGTCCTGGA TTCCGGAGGGAGTGGTACGGTCCAACGGCCATACCTCTGCGGATCAGCTAAGTGAGGAGAAGGAGAAGGG GTTGTGTGGCTGTTGAGACGCCAAGCGAGCCCCGTGGCAGCAGCGCATGACCCCTGGTTGCCGCCAGCTC CGCTGTAGAGACCAAGCCGGCAGCTCGATGAGATGAGCAAACAGCCCCTCCTATGCCAGGTGCCTCTCCT CGCCTCCAGGAGGGTCACCTCGGGCATCCATCTGTAACGCGCTGCAGCTTTGTATGATTTAGTCCATGAA TCGGAAGAAATACTTCACAGAGCCAAAAGTGTCTTATAAAAGCCCACGGTGGCGGGTCATTTAGGGATCC AAGATTTCAGCCATGAAAGATTTCATCTGTTATGTAGAACGCTGATGGTCCTGTCGCCTGCCTTAACACA GTCGCCTGCCTCAACACAGGACAGCCCTAGAGTCTCATTGAAGACATTAAGATCTTTGAGCAAGGAAAAG CTAGCACGGTCTTGAGAATCCAGATTTCAAAATCCATACTGTAGACTATCACTCTCAAACACGTGAATGT CAATAAGTGTGTACACACAGATCAATGTCTTTCAGCATTGCTTTTTAACATAGCCTTTCCTTTGACATGA GCATAAACAGATGGCGGCCATACCTATAGACTCTGAATAGATCGGATTGACTCACTGTGAGTTTGGGGGT GGGGGGAAGGGTTTATGGAGCAGGGGTGGGTGAAGTAGGGATCAGTCAGATTGTCTGCTGTTAGCCGAGG GGTCATGAAGACTCTTGTGTCTATCCATGGTCTGTTGGGTTCAGTGCACCTCTGAACATCGCTCATGCTT CTACAGTATGTATGTTTGCATGTATGTATGTATGGTCTCTTCATATGTACGTATGTTTGGTCTGGCACCT CAGGATCTGCTTTCACTTGTCTGGAGACTGCCAATCTGACTTTTCCCATCAAGTTTTGGTGGTGGTGGTG TTTCCCAGTTTGTTTTCTCCCCCCCCCTTCCCCCATTAATGTAACTGAAGGAATCTCTTTTTAATTAAAC GGCGTGTTTATTTGCTAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC008173
Insert Size	666 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC008173, AAH08173
RefSeq Size	1995 bp
RefSeq ORF	666 bp
Locus ID	11891
Gene Summary	The protein encoded by this gene is a member of the Rab family of proteins, which is the largest family within the Ras superfamily of GTPases. This gene product is thought to regulate vesicular transport, together with its specific effectors. Mutations in this gene cause several defects, including actin-based melanosome transport defects and immunodeficiency. Mutations in the human ortholog of this gene are associated with Griscelli syndrome type 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

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