Pias2 (BC005596) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Dib, ARIP3, PIASxbeta, SIZ2, PIASxb, PIASxalpha
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC005596 TCTCCCTCGGCGTGGGGAGAAGCGCGCTCTTTTGGGAACGCCGCCTTCCGTAGTCGGCCTCCTACCCAGA GACTTGGTTTGTAACCATGAATATGGTTTCTAGTTTTAGGGTTTCTGAGTTACAAGTGTTACTGGGCTTT GCTGGACGGAATAAAAGTGGGCGCAAGCATGACCTCCTGATGAGGGCGTTGCATTTATTGAAGAGTGGCT GCAGCCCTGCGGTTCAGATTAAAATTCGAGAATTATATAGACGCCGATACCCACGGACACTTGAAGGACT TTGTGATCTTTCCACAATCAAATCATCGGTTTTCAGCTTGGATGGTAGCTCATCACCCGTAGAACCTGAC TTGCCTGTGGCTGGGATCCACTCGTTGCCTTCGACTTCAATTACACCTCATTCACCGTCATCTCCTGTTG GTTCTGTACTGCTGCAAGACACTAAGCCCACCTTTGAGATGCAGCAGCCGTCTCCGCCCATTCCTCCTGT CCATCCTGATGTGCAGTTAAAAAACCTGCCTTTCTATGATGTCCTTGATGTTCTCATCAAGCCCACGAGT TTAGTTCAAAGCAGTATTCAGCGGTTTCAAGAGAAGTTTTTTATTTTTGCTTTGACACCTCAGCAAGTTA GAGAAATATGTATTTCAAGGGACTTTTTGCCGGGTGGCAGGAGAGACTACACAGTCCAAGTCCAGCTGCG ACTTTGCTTGGCAGAGACCAGTTGCCCTCAAGAAGATAACTATCCCAATAGTTTGTGTATAAAAGTCAAT GGGAAACTCTTTCCTTTGCCTGGCTATGCGCCACCACCTAAAAATGGGATTGAACAGAAGCGCCCTGGAC GCCCCCTGAACATTACGTCTTTAGTTAGGTTGTCTTCAGCTGTGCCAAATCAGATTTCTATTTCTTGGGC ATCTGAAATTGGAAAGAATTACTCCATGTCTGTGTATCTTGTACGACAGCTTACATCAGCCATGTTATTA CAGAGATTAAAAATGAAAGGTATTAGAAATCCTGATCATTCCAGAGCACTAATTAAAGAAAAACTTACTG CAGACCCTGATAGTGAAATTGCTACAACTAGTCTTCGAGTGTCCTTGATGTGCCCTTTAGGAAAAATGAG GCTGACAATCCCGTGCCGTGCAGTGACTTGTACACATCTGCAGTGCTTTGATGCTGCCCTCTATCTTCAG ATGAATGAGAAAAAGCCCACCTGGATCTGTCCTGTTTGTGACAAAAAGGCTGCCTATGAGAGTCTGATAC TAGATGGGCTTTTTATGGAAATTCTCAATGACTGTTCTGATGTGGATGAGATCAAATTCCAGGAAGATGG TTCTTGGTGTCCCATGAGACCTAAAAAAGAAGCTATGAAAGTAACCAGCCAGCCCTGTACAAAAGTAGAA AGTTCAAGTGTCTTTAGTAAACCTTGTTCAGTGACTGTAGCCAGTGATGCAAGCAAGAAGAAGATAGATG TTATTGATCTAACAATAGAGAGCTCTTCTGATGAAGAGGAAGACCCTCCCGCCAAAAGGAAATGCATCTT TATGTCAGAAACACAAAGCAGTCCAACCAAAGGGGTTCTCATGTATCAGCCATCTTCTGTAAGGGTGCCC AGTGTGACTTCAGTTGATCCTGCTGCTATTCCACCTTCATTAACAGACTACTCAGTACCATTCCACCACA CGCCAGTGTCAAGCATGTCATCAGATTTGCCAGGTTTGGATTTTCTTTCCCTTATTCCAGTTGATCCCCA GTACTGTCCTCCTATGTTTTTGGATAGTCTCACCTCACCCTTAACAGCAAGCAGTACGTCTGTCACCACC ACCAGCCCCCATGAAAGCAGTACTCACGTTAGTTCATCCAGCAGCAGGAGTGAGACAGGGGTCATAACCA GCAGTGGGAGGAACATTCCTGACATCATCTCTTTGGACTAAAGGCGGACATACTTGATTCTGGGAATCAT TCATCAAAACTCTTTCTTGGATGTTTAGTCCATGGAAGCTGATTTTTGCAACTTAATATTTATTGAAATG TCAAATGGATTCTTTGCTCTCTGAGAGATGAACATCGATGACTTCAGCAAGAACCAGATGACATGCAAGG ACTTTTCTTATGCTGTACATGGAGCATCAGATCTATCTCATCCACAACGGTGGCTTGTGGGACAGTTGAT TTCAGTTTCTACGTTACTGGATGGATTCTACACACCAGTTAATCTTTTGTTACAATAAACAGCAATAAAG TTATGTAAATAGTTAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC005596
Insert Size	1839 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC005596, AAH05596
RefSeq Size	2269 bp
RefSeq ORF	1839 bp
Locus ID	17344
Gene Summary	Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIASx-beta, but not isoform PIASx-alpha, promotes MDM2 sumoylation. Isoform PIASx-alpha promotes PARK7 sumoylation. Isoform PIASx-beta promotes NCOA2 sumoylation more efficiently than isoform PIASx-alpha (By similarity). Sumoylates PML at'Lys-65' and 'Lys-160' (By similarity).[UniProtKB/Swiss-Prot Function]

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