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Dnase1l3 (NM_007870) Mouse Untagged Clone
CAT＃: MC200910
Dnase1l3 (untagged) - Mouse deoxyribonuclease 1-like 3 (Dnase1l3), (10ug)
CNY 2,400.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Dhp2; DNasegamma; Lsd |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC012671 sequence for NM_007870
CCACGCGTCCGATTGAAACGCGTGATGGTGAGTTCCTCAGAGAAGTGAAAGTGACCTAGAGGGATCCAGT AATTCCTGTTATCAGCCTGCTTTATAAGTCAGTGAGCCAGGCACTGTCTTCATCCAGCCTGAAGTCCCAG GAGTGCAAAGATGTCCCTGCACCCAGCTTCCCCACGCCTGGCCTCCCTGCTGCTCTTCATCCTTGCCCTC CATGACACCCTGGCCCTAAGGCTCTGCTCCTTCAATGTGAGGTCCTTTGGAGCGAGCAAGAAGGAAAACC ATGAAGCCATGGATATCATTGTGAAGATCATCAAACGCTGTGACCTTATACTGTTGATGGAAATCAAGGA CAGCAGCAACAACATCTGTCCCATGCTGATGGAGAAGCTGAATGGAAATTCACGAAGAAGCACAATATAC AACTATGTGATTAGTTCTCGACTTGGAAGAAACACGTACAAAGAGCAGTATGCCTTCGTCTACAAGGAGA AGCTGGTGTCTGTGAAGACAAAATACCACTACCATGACTATCAGGATGGAGACACAGACGTGTTTTCCAG GGAGCCCTTTGTGGTTTGGTTCCATTCCCCCTTTACTGCTGTCAAGGACTTCGTGATTGTCCCCTTGCAC ACAACTCCCGAGACCTCCGTTAAAGAGATAGATGAGCTGGTCGATGTCTACACGGATGTGAGAAGCCAGT GGAAGACAGAGAATTTCATCTTCATGGGTGATTTCAACGCCGGCTGTAGCTATGTCCCCAAGAAGGCCTG GCAGAACATTCGTTTGAGGACGGACCCCAAGTTTGTTTGGCTGATTGGGGACCAAGAGGACACTACGGTC AAGAAGAGTACCAGCTGTGCCTATGACAGGATTGTGCTTTGTGGACAAGAGATAGTCAACTCCGTGGTTC CCCGTTCCAGTGGCGTCTTTGACTTTCAGAAAGCTTATGACTTGTCTGAGGAGGAGGCCCTGGATGTCAG TGATCACTTTCCAGTTGAGTTTAAGCTACAGTCTTCAAGGGCCTTCACCAACAACAGAAAATCTGTTTCT CTCAAAAAGAGAAAAAAAGGCAATCGCTCCTAGGTATCACGCTGCCTTTTTTTTTTTTTTTTTGCCACTC CTTGCAAATGGCTCTCATAGGTCTAAGCTATCTGCATGCTCAGGAATTAAGACTGGCCAAGCTGCTTTCA TTGTCCACTTGAGTTAATTTTGCCTGGAACCAAGCTGGGAGGAGAGTCTTCTGTTACATCACCTTGATCC CCAATATCATCACTGGTACCCTGCGAAACATTGCCACTATTTAGGCAACAGGACAGATTTGGCATGGTAT GATACTTTATGATGGAGGCAATGAAACTCAGTTGCCTGGGGTCATGCGTGGAAGAAGAGTGAAGGACACT TCCACTGTGCTGTCCTGCCCGTCCCTACTCACAGCCCACCCCTACCCACACCTCACTCCTATCCACACCT CACTCCTATCCACACCTCACCCCTAGCTACAGCCCACCTGTGCTCACAGCGCTGTGCTTAAGCCATCAGC ATGTCCTGAGCTCAGTCTTCGGATATATGAAGGTTTTCATTATCTGACAGCCAGCCTCACTTTGGATGCT CCAACAGTGTCTTCCCTCCCTTTCTCCCTTCCTCTTCCTCCTGTGCCCCTCCCTGAAAGGGCTCAACCTT GCGTGCCTGTCCTGTTCTAACTGTCCCCAGTCACATATCCCATGTGCAACACTGACCACACAGTGTCTGT CACCACGGCCAGCACTGCAGCTCGCCCCAGCACAAGCCCCCCTGGCTGGCTTGGACCTGAGTGTTTGCTC CCTTCCTGCCACTCCTGGAATCTGCAATGTGGCGCCATCTTGCTTGTATGCAGGGCACCCGTTTTTGTGC ATTTCGCTTTGTTTTCCCGGATATTCTTTACTCTTGCACATGAGCACCTGTACTTAGGAACAGTGGAGAA CAGTGACCCGTCTGGCCCGACTCCCAGTCTCAGAAGCAACCCCTCCACACAGCTGTGGGCTCCCCAAAGG CGTCTGCACACAAGCAAGCACCATGTGTAACCTGAAGAAATACAAAGTCTATTACACTGTAAAAAAAAAA AAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_007870 |
| Insert Size | 933 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC012671, AAH12671 |
| RefSeq Size | 2110 bp |
| RefSeq ORF | 933 bp |
| Locus ID | 13421 |
| UniProt ID | O55070 |
| Gene Summary | Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends (By similarity). Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA (PubMed:15796714, PubMed:19154352, PubMed:12095301). Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis. The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells (PubMed:12050166, PubMed:15167901, PubMed:17218958, PubMed:24312463). Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity (PubMed:15796714, PubMed:27293190). Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs) (PubMed:29191910). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (PubMed:29191910). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (PubMed:29191910).[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204391 | Dnase1l3 (tGFP-tagged) - Mouse deoxyribonuclease 1-like 3 (Dnase1l3) |
CNY 2,850.00 |
|
| MR204391 | Dnase1l3 (Myc-DDK-tagged) - Mouse deoxyribonuclease 1-like 3 (Dnase1l3) |
CNY 2,400.00 |
|
| MR204391L3 | Lenti ORF clone of Dnase1l3 (Myc-DDK-tagged) - Mouse deoxyribonuclease 1-like 3 (Dnase1l3) |
CNY 4,750.00 |
|
| MR204391L4 | Lenti ORF clone of Dnase1l3 (mGFP-tagged) - Mouse deoxyribonuclease 1-like 3 (Dnase1l3) |
CNY 4,750.00 |
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