Tfap2a (NM_011547) Mouse Untagged Clone

CAT#: MC200948

Tfap2a (untagged) - Mouse transcription factor AP-2, alpha (Tcfap2a), transcript variant 1, (10ug)



  "NM_011547" in other vectors (4)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms A; AP; AP-2; Ap-2 (a); Ap2; AP2alpha; Ap2tf; Tcfa; Tcfap2a
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC018226 sequence for NM_011547
GCCGATTTGCACAGGAAGAGTGGTTCCTAATGTTTTATTTTGATGCATGTACGACACCGCATGGAGAGTC GCAGGAGCTCATGGGTCTGCGGTTTGGCTCACTCCAGAAGGGGTTGTGCATCCCCATCCGAATTCTGTGT TGTTGTCCACGTTGCACCTGGGAAACCTTACAGTCCAAACGCCCGGATGAATAGTCAGTCCGATTCAGCT TCATACAAACCGGAGGAAAATCATTTAACCCCCGGATCTGTCGAGTAACCTCTTCAGGCCCGCACTTTGC GCTAACCCAGAGAGTAGCTCCGCTGGGGTGCGAGGCGCAGAGGGGCAAATCCGATCACGCCGATCCATGA AAATGCTTTGGAAACTGACGGATAATATCAAGTACGAAGACTGCGAGGACCGTCACGACGGCACCAGCAA CGGGACGGCAAGGTTGCCCCAGCTGGGCACTGTAGGTCAATCTCCCTACACCAGCGCCCCGCCGCTGTCC CACACCCCTAATGCCGACTTCCAGCCTCCCTACTTCCCCCCGCCCTACCAGCCTATCTACCCCCAGTCGC AAGATCCTTACTCCCACGTCAACGACCCCTACAGCCTGAATCCTCTGCACGCCCAGCCGCAGCCGCAGCA CCCGGGCTGGCCCGGCCAGAGGCAGAGCCAGGAATCTGGGCTCTTACACACACACCGGGGCTTACCCCAC CAACTGTCGGGCCTGGACCCTCGCAGGGACTATCGGCGGCACGAGGACCTCTTGCACGGCCCGCACGGGC TCGGCTCTGGACTCGGGGACCTCCCGATCCACTCCTTACCTCACGCCATCGAGGACGTCCCGCACGTAGA AGACCCGGGTATTAACATCCCAGATCAAACTGTAATTAAGAAAGGCCCCGTGTCCCTGTCCAAGTCCAAC AGCAATGCTGTCTCCGCCATCCCTATTAACAAGGACAACCTCTTCGGTGGCGTGGTGAACCCCAACGAAG TCTTCTGTTCAGTTCCGGGTCGCCTGTCGCTCCTCAGCTCCACCTCGAAGTACAAGGTCACGGTGGCGGA AGTACAGCGGCGCCTCTCACCGCCCGAGTGTCTTAACGCGTCGCTCCTGGGCGGAGTACTGCGGAGAGCG AAGTCTAAGAATGGAGGGAGATCTTTAAGAGAAAAACTGGACAAGATAGGATTGAATCTGCCAGCAGGGA GACGTAAAGCTGCCAACGTTACCCTCCTCACGTCACTAGTGGAAGGAGAAGCCGTCCACCTAGCCAGGGA CTTTGGGTACGTGTGCGAAACTGAATTTCCTGCCAAAGCAGTAGCAGAATTTCTCAACCGACAACATTCC GATCCCAATGAGCAAGTGGCAAGAAAAAACATGCTCCTGGCCACAAAACAGATCTGCAAAGAGTTCACTG ACCTGCTGGCTCAGGACCGATCTCCCCTGGGGAACTCGCGGCCCAATCCTATCCTGGAGCCTGGCATCCA GAGTTGCTTGACCCACTTCAACCTCATCTCCCATGGCTTCGGCAGCCCGGCGGTGTGTGCAGCGGTTACG GCCCTGCAGAACTATCTCACCGAGGCCCTCAAGGCCATGGACAAAATGTACCTCAGCAACAACCCCAACA GCCACACGGACAACAGCGCCAAAAGCAGTGACAAAGAAGAGAAACACAGAAAGTGAGGCTCTCCTCCCGC CCCGCCCCCGCCCGCCTCACCGGCCCGCGTCCCCCCACTCGCCCCTCCCCCACCCGGCAGGTGACAGCTC CGGGACCAGCCACTCCTACTGCTGCTGCTACTCTGCGTTGCCGCGCCGCCCTTCGTCCCCGGAGTCCTCC GGATTGCTCTCTCGACTGCCAGTGGGGCAGCCGCTGCCTACTCAGCGCCTTGCCTCAACTTCCCCATCAG CACCAACACCCCCTTTACCCTCGTGTGGAGCCTAAGAGAACAGAACAGGTCGTGAAGCCAGCAAAGAAAA GTTCTGTCGCGTTTGTGAACCTTTTTTTTTAAAATCAAATCCACAACAAACATTAAAACTTTTTTTTTAA AAAGGACGTTAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_011547
Insert Size 1314 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC018226, AAH18226
RefSeq Size 2056 bp
RefSeq ORF 1314 bp
Locus ID 21418
UniProt ID P34056
Gene Summary This gene is a member of the activator protein 2 (AP-2) transcription factor family. The protein encoded by this gene can act as both an activator and repressor of gene transcription, and plays an important role in early embryogenesis, specifically in cranial development. This protein forms both homodimers and heterodimers, and binds to a GC-rich consensus sequence found in some promoters and enhancers. Disruption of this gene causes perinatal death, with neural tube, craniofacial, and limb mesenchyme defects. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms. [provided by RefSeq, Sep 2014]
Transcript Variant: This variant (1a) represents the shortest transcript and encodes the longer isoform (1, PMID: 7750631). Both variants 1a and 1b encode the same isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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