Mdk (NM_001012336) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Mek; MK
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC012244 sequence for NM_001012336 AAACCGAACTCCAGGACCAGAGACCCAGAGATCAGAGGATGCAGCACCGAGGCTTCTTCCTTCTCGCCCT TCTTGCCCTCTTGGTGGTCACGTCCGCGGTGGCCAAAAAAAAAGAGAAGGTGAAGAAGGGCAGCGAGTGT TCGGAGTGGACCTGGGGGCCCTGCACCCCCAGCAGCAAGGACTGCGGCATGGGCTTCCGCGAGGGTACCT GTGGGGCCCAGACCCAGCGCGTCCATTGCAAGGTGCCCTGCAACTGGAAGAAGGAATTTGGAGCCGACTG CAAATACAAGTTTGAGAGCTGGGGGGCGTGTGATGGGAGCACTGGCACCAAAGCCCGCCAAGGGACCCTG AAGAAGGCGCGGTACAATGCCCAGTGCCAGGAGACCATCCGCGTGACTAAGCCCTGCACCTCCAAGACCA AGTCAAAGACCAAAGCCAAGAAAGGAAAAGGAAAGGACTAAGTCAGGAGGCCAGAGAGCCTCCGGCCTCG CCTGGAGCCTGAACGGAGCCCTCCTCTCCCACAGGCCCAAGATATAACCCACCAGTGCCTTTTGTCTTCC TGTCAGCTCTGTCAATCACGCCTGTCCTCTCACGCCCACACCAAGTGCCCAAAGTGGGGAGGGACAAGAG ATTCTGGAAAGTGAGCCTCCCCATACCCTCTTTTGTTCTCCCCACCCTGATACTTGCTATTAAGAAATGA ATAAAATAAACTCACTTTTTTCCAATAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	NM_001012336
Insert Size	423 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC012244, AAH12244
RefSeq Size	753 bp
RefSeq ORF	423 bp
Locus ID	17242
UniProt ID	P12025
Gene Summary	This gene encodes a secreted growth factor that belongs to the pleiotrophin/midkine heparin-binding protein family and functions in a variety of biological processes. The encoded cytokine promotes the growth, differentiation, survival and migration of several target cells including leucocytes involved in inflammation. This protein plays a role in the formation of scar tissue and intraperitoneal adhesions, and promotes neurite outgrowth and neuron survival. The protein encoded by this gene is associated with obesity and inhibition of insulin signaling in fat cells. A pseudogene of this gene is present on chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] Transcript Variant: This variant (3, also known as MK2; PMID 2345177) differs in the 5' UTR compared to variant 1. Variants 1 through 4 encode the same isoform (a).

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