Pip5k1a (NM_008847) Mouse Untagged Clone

CAT#: MC201475

Pip5k1a (untagged) - Mouse phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha (Pip5k1a), (10ug)



  "NM_008847" in other vectors (4)

CNY 4,072.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms PI4P5K-I[a]; PIP5K1-alpha; PIP5KIalpha; Pipk5a
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC031774 sequence for NM_008847
CCACCCTGAGCCGAGCGGTGGAGTTGGGGATAAACAGGCAGTGGCTGGGGAGGTGAGGGAGGGGGGGCAT TCGCTTTTGGGGGCACCTCAGGTGCCTAGGTTTTCCTTTAGGTTTGTTCTATCCGAGACGCGGGACTCCC GGGGCCCGGCTCAAGTGTTCCTGTCTGGTAAGAGAAGGTAGCTTTGGCGGAGAGCAGTTCAAGTTGCTAG GAAGGTGCGAGACTGCAGCTCTCCCCCATGCCGGGCAAGTGGTGCGGTCCTGAGCTTGTCCTGGAGCCTG AACAACGCCTGTGAGAAGCCCGCCGAACGCGGGTTCTGTGAAGAGACGTGGGGAAGATTCGATTCCGAGA AAAGGAAAAGCCGGATTGAAAGGGAGCGAAGCCGCTGAGGGGGAGGGGGCTGCCAAGATGGCGTCCGCCT CCTCAGGGCCAGCGGCGGCCGGGTTTTCATCCCTTGATGCCGGGGCCCCTGCTGGTACCGCAGCAGCATC TGGAATCAAGAGAGCCACAGTATCTGAGGGACCTTCCGCCTCTGTCATGCCTGTTAAAAAAATAGGCCAT CGAAGTGTTGATTCCTCTGGAGAGACTACATACAAGAAGACAACTTCATCAGCCTTGAAAGGTGCCATCC AGTTAGGCATCACTCACACTGTGGGCAGCCTGAGCACCAAACCAGAGCGTGATGTCCTCATGCAAGACTT CTACGTGGTGGAGAGCATCTTTTTCCCCAGTGAAGGCAGCAACCTGACACCGGCTCACCATTACAATGAC TTTCGATTCAAGACCTATGCGCCTGTTGCCTTCCGTTACTTCAGAGAGCTCTTTGGCATCCGGCCTGATG ATTACTTGTACTCCCTTTGCAGTGAGCCATTGATTGAACTCTCCAATTCTGGAGCTAGTGGTTCCCTCTT CTATGTGTCCAGTGATGATGAATTCATCATTAAGACCGTCCAGCATAAAGAAGCAGAATTTCTGCAGAAG TTGCTTCCAGGATACTACATGAATCTTAACCAAAACCCTCGTACTTTGCTGCCCAAATTTTATGGATTGT ACTGTGTGCAAGCAGGCGGCAAGAACATACGAATTGTGGTGATGAACAATCTCTTGCCTCGGTCAGTCAA AATGCATATGAAATATGACCTGAAGGGTTCAACTTACAAGCGGCGGGCTTCTCAGAAAGAACGAGAGAAA ACTCTCCCCACCTTTAAAGACCTGGACTTCCTACAAGATATCCCTGACGGCCTATTTTTGGATGCTGACA TGTACAGTGCTCTGTGTAAGACTCTACAGCGTGACTGTTTGGTGCTGCAGAGCTTCAAGATAATGGACTA CAGCCTGTTGATGTCCATCCACAACATGGACCATGCACAGAGGGAGCCCACGAGCAATGACACACAGTAC TCGGCTGACACGCGCAGACCAGCCCCACAGAAGGCGCTCTATTCCACAGCTATGGAATCCATCCAGGGCG AGGCTCGACGGGGCGGCACTGTGGAGACTGAGGACCACATGGGTGGCATCCCTGCCCGGAATAACAAAGG AGAAAGGCTCCTGCTTTATATTGGCATCATTGATATTCTTCAGTCCTACAGGTTTGTTAAGAAGTTGGAG CACTCTTGGAAAGCACTGGTCCATGATGGGGATACGGTGTCAGTGCATCGTCCAGGCTTCTATGCTGAGC GGTTCCAGCGTTTCATGTGCAACACAGTGTTCAAGAAGATTCCCTTGAAGCCTTCTCCTACCAAAAAGTT TCGGTCTGGCCCGTCTTTCTCTCGGCGATCAGGCCCCAGCGGCAACTCCTGCACCTCCCAGCTGATGGCC TCTGGGGAACACAGAGCACAAGTGACCACCAAGGCGGAAGTGGAGCCAGATGTACACCTTGGGCGTCCTG ATGTCTTACCTCAGACTCCACCTTTGGAGGAAATAAGTGAGGGTTCACCTGTTCCTGGCCCCAGTTTCTC ACCTGTAGTTGGACAACCTTTGCAAATACTAAATTTGAGTTCAACCTTGGAAAAGCTTGATGTTGCAGAG TCAGAGTTCACCCACTGAGCAGTGAGCCTCAGAAGACCTGAAACAAGACTCTGCTGTCGCTTTGATCCCA AGATGTCAGCCCTTGCCCCAGGAATGCTGAATCGTCTTCTTGGTCATCAACAGAGGAGAGAATGTGGAGC TAGCAGGGCTCTGACACTGTCTCCCCTTCCTCTGGGCAGTAGTGCCTTTGACAGTTGAGGACAGCATCGT CCCCACCACTCCAGAAGTGGGTGGCGTGAATTTTCCACTGCCTGCCATGGCTTCTATAGCTGAATATTTT TCAAACCCCCATTCTTTGTGCTGACATGGGGTTGCTGGACTGGTGGTTTCCTCCTGTTTACCTTTCTCCT GGAGCTGGACTCTTCATTTCCTCAGGACGGACTGGCTGGACATTGTTCCTGCCTTGGCTCTCCCGCTGGT CTTTGGAGACTCCCAATGATTTGTATAAAGGTGTTTGGGGGAAAGTAAGGATCATCCTTACTCCCAACCT TCTTTTCCTTAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_008847
Insert Size 1641 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC031774, AAH31774
RefSeq Size 2553 bp
RefSeq ORF 1641 bp
Locus ID 18720
UniProt ID P70182
Gene Summary Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs (PubMed:10679324, PubMed:18772378, PubMed:19153220, PubMed:20622009, PubMed:8798574). Positively regulates insulin-induced translocation of SLC2A4 to the cell membrane in adipocytes (PubMed:27739494).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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