Mgmt (NM_008598) Mouse Untagged Clone

CAT#: MC202089

Mgmt (untagged) - Mouse O-6-methylguanine-DNA methyltransferase (Mgmt), (10ug)



  "NM_008598" in other vectors (6)

CNY 2,400.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Agat; AGT; AI267024
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC031888 sequence for NM_008598
AAAACTTGTGTACCGTTCCCCGTTGCTGTCTGCAGTTTGCAAGCTGGAACTTGGCAGAATGGCTGAGACC TGCAAAATGAAATACTCAGTGTTGGACAGCCCTTTGGGGAAGATGGAGCTGTCTGGCTGTGAGCGAGGCC TGCATGGGATACGGTTGCTCAGTGGGAAGACCCCAAACACTGACCCCACAGAGGCCCCAGCTACTCCTGA GGTGCTCGGTGGGCCAGAGGGAGTTCCAGAGCCTCTGGTGCAGTGCACAGCCTGGCTGGAAGCCTATTTC CGTGAACCCGCAGCCACAGAGGGGCTTCCCTTGCCTGCTCTCCATCACCCTGTGTTCCAGCAAGATTCAT TCACCAGACAGGTGTTATGGAAGCTGCTGAAGGTTGTGAAATTCGGAGAAACGGTTTCTTACCAGCAATT AGCAGCCCTGGCAGGCAACCCCAAAGCGGCTCGTGCAGTAGGAGGAGCAATGAGAAGCAATCCGGTCCCC ATCCTCATCCCCTGCCACAGGGTGGTTCGCAGTGACGGTGCCATCGGCCATTACTCCGGAGGAGGGCAGG CTGTGAAGGAGTGGCTTCTGGCCCATGAGGGCATCCCGACCGGACAGCCAGCCTCCAAGGGCTTGGGTCT GACTGGGACCTGGCTCAAGTCATCCTTCGAGTCGACCAGCTCTGAGCCGTCTGGCCGAAATTGAGTAACC GTTTGAATGACACATAGATGTAACGCGTGTCGGAAGCGGATGTGTGGTGGCACCACTATATTAAAAGAGC TGCAAGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_008598
Insert Size 636 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC031888, AAH31888
RefSeq Size 815 bp
RefSeq ORF 636 bp
Locus ID 17314
UniProt ID P26187
Gene Summary Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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