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Lpar1 (NM_010336) Mouse Untagged Clone
CAT＃: MC202097
Lpar1 (untagged) - Mouse lysophosphatidic acid receptor 1 (Lpar1), transcript variant 1, (10ug)
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI326300; Edg2; Gpcr26; Kdt2; lpA1; vzg-1 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC025425 sequence for NM_010336
CCACGCGTCCGCTCAGCCCGGGAGGGAAGTCGCCGCAGCCGCCCGGGCGGCCCTCCCGACCTTTGGCCAG GCTTACAGTTCCTCCTAGCATGACCGAGATCTGATCAGCCAACCCGCGCATTGCTTTTTGTGCCTGGCAC TGCAGTGCAGGGGGCCTCTTCATCGCCCCAAACTACAGCACTGTCATGGCAGCTGCCTCTACTTCCAGCC CTGTAATTTCACAGCCCCAGTTCACAGCCATGAACGAACAACAGTGCTTCTACAATGAGTCTATCGCCTT CTTTTATAACCGGAGTGGGAAATATCTAGCCACAGAATGGAACACAGTGAGCAAGCTGGTGATGGGACTG GGCATCACTGTTTGCGTGTTCATCATGTTGGCCAATCTCCTGGTCATGGTGGCAATCTACGTCAACCGCC GCTTCCATTTCCCTATTTATTACTTGATGGCCAACCTGGCTGCTGCAGACTTCTTCGCTGGATTGGCCTA CTTCTACCTGATGTTCAATACAGGACCTAATACCCGGAGACTGACTGTTAGCACGTGGCTCCTCCGGCAG GGCCTCATTGACACCAGCCTGACAGCTTCTGTGGCCAACCTGCTGGCTATTGCTATCGAGAGGCACATCA CGGTTTTCCGCATGCAGCTCCATACACGAATGAGCAACCGGCGCGTGGTGGTGGTGATTGTAGTCATCTG GACTATGGCCATTGTGATGGGTGCTATACCCAGTGTGGGCTGGAACTGCATCTGTGATATCGATCACTGT TCCAACATGGCACCCCTCTACAGTGACTCCTACTTAGTCTTCTGGGCCATTTTCAACCTGGTGACCTTTG TGGTCATGGTGGTTCTCTACGCTCACATCTTTGGCTATGTTCGCCAGAGGACTATGAGGATGTCTCGGCA TAGTTCTGGACCCAGGAGGAATCGGGACACCATGATGAGCCTTCTGAAGACTGTGGTCATTGTGCTTGGT GCCTTTATTGTCTGCTGGACTCCGGGATTGGTCTTGTTATTGCTGGATGTGTGCTGCCCGCAGTGCGATG TCCTGGCCTATGAGAAGTTCTTCCTCCTCCTGGCCGAGTTCAACTCTGCTATGAACCCCATCATCTACTC CTACCGCGACAAAGAGATGAGCGCCACCTTCAGGCAGATCCTGTGTTGCCAGCGCAACGAGAACCCTAAT GGCCCCACGGAAGGCTCTGACCGCTCTGCCTCCTCCCTCAACCACACCATTCTGGCTGGAGTTCACAGCA ACGACCACTCTGTGGTTTAGAAGGAAGCCAGCCGGCCTCTGTGGATCTGTGAACCCCACCCTACCCCCCA TTGCCAGGGCAAGGTGGGGAGCCAGAGGAGATGAGGACACTCCTGTACTTAACACTAACCAATGGCAGTA TTTGTCCCTAGACCCAAGAGACTTGAGGATGAATTTATTTGGCAGGCCCCATCTTCTCCTTTGGAAAACA GAAGGGGACCGTCTTGTGGTGGAATTGAGAAATGGACTCTGGGGTGACCGTGTAGCATTCACTAACTAGA CTTAAAAGATTTTATGTGGTTTGGCTTAAGCCAGGAAAAAAAAATCTGCTGAATTGAGTATACAATCGAG TATACACAGGCTTCCCCTTTAAAGAACAAACAATACATTGCATTTATTAATGAGTATGTTTATGCCTGAC AGCATGTTTGTGATCGAAAAGACTGCTAAACTGACATAGATGAGTTGTTTTTTTTTTTTTGTTTTTTGTT TTTTTTTACATGATGGAGGAAAAGTATAAATTAGAATGATTTTTGTGTTTGTTTAGAAAGCAAGCATGTG GTGTGTGTATTCAGTATGCCTTTCTTTAAAGATAAAAGGCCACTATTTTAAATCTTCTAGGGAATAGAAG AATCTAGTAAAAACCAGTATTCATTTAGGCTACAGGAAAAACCATATCCTAATCAATTACCTTTTAATTA AAGTAATGAAATATACATGAAAGGCAAAGTAATGTGAGCTTGTCACCCAAAGAGTGTGTGCTCTCCAAAC GCTGGAGGAGATGAAGCTGTAGCGTTGTCCCTGCATAGTGAAGATACCCACGTGCGTTCTCAGTGCCAGA CCCTCAGTGGGACTTGTTTTAAAGCCTGTGGTTTTCCAAGTTAGAAAATAATACCTACTTACTATAGAAA ACTTGAAAATTGCAGAACTGTGTGAAAAAAGAAAAAAGAAAGAAAGATTGCTGATATACGACCTGGAAGT AGCCATCTTTCCCTGCTCACCCACGTATGCCTATAGACATATATTATATACCTTTTTTTTTTTTTACATA ATCGGGTTGATATTGTAAAATGTTTTGTAACTTTTTCTTTCAGAATTGCCAGGTTTTTCTATGGCATTTT TTTTCAAAAACAGTGTTAGCGGCTATATAATATTCCATTTAATGGATGCAGCTCAGTTTATTTAACCATT CCTGTATTGTTGACTATGTTTTTAATTTTTTTCATTATTATAATATTGGAAGAATCTTGTGTATATAAAA CTTTGCCAGTATCATTGATGATTTACTTGGGCTCCATTCCCATGAAGGACCTACTTTTAAAATGTGAAAT GTCCTCATGTGTTGTCACTTTATAAAAAGGGATATTCTACCCTCAAACTGCAGGGGTGACCATGCTGACA AAAAGCTCAGAAATTACCTTTTTACAAAAGAAACACAGTGGCTACTTTAGTTGCGAATGGGTTCTTGACA AGATGTTTCCCAATAACCCAGACCCTTAACATAACTAGCAAATTTACTTTAGTAGGAACTGGTGATCCCT TTCTGGACACAAGAAAACACAAAGACATCAAGCATCAGAGTGAATCCAGCAATGCAGTACCAAGCAGCCT GGGTGGGGTGTGCTAGCAACTGGCTGCTGTGTGGTCTCCAGCAATCACCCAATGGACACCCTGTGTTCAG AGATGCCCAGTACAGTGTCCTGGAGACTAAGGTCTCTAATGTCATGCTATGAGGAACAAGGAGGAAGAAG AATAGTCCTGAAGCAGGTGTGGAGAGGTCAGGAGGACGTCTGAGAGAAGGCAGTCCTGCCAAGAGTGGCA TTCTAGGGAGGAGGAAGGCAGGGTGTGGCTTGTGTCTGGACAGTCAGTGGTAGGCATGTGACATTTGCCT GGTGGAAAAAAAATAAGTAGGCGGAGATCCAGTTAGCAGGACAAAGATTTTGCTCGAGGATTCACAAAAT GCAAAGAAATTTATACTGGAAATGAGTGAAACGAACTTGGACTTTTATTGATGCCCTTTTTATGGGGGAG GTAAGGACTATTTGAATTGAAAAGCATACTAATTGAACCTTAATAAATCATTCTCAATCAAAAAAAAAAA AAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_010336 |
| Insert Size | 1095 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC025425, AAH25425 |
| RefSeq Size | 3365 bp |
| RefSeq ORF | 1095 bp |
| Locus ID | 14745 |
| UniProt ID | P61793 |
| Gene Summary | Receptor for lysophosphatidic acid (LPA) (PubMed:11087877, PubMed:18066075). Plays a role in the reorganization of the actin cytoskeleton, cell migration, differentiation and proliferation, and thereby contributes to the responses to tissue damage and infectious agents. Activates downstream signaling cascades via the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins (PubMed:8922387, PubMed:9600933, PubMed:11040035, PubMed:18157949, PubMed:18066075, PubMed:23478264). Signaling inhibits adenylyl cyclase activity and decreases cellular cAMP levels (PubMed:11040035, PubMed:12215548). Signaling triggers an increase of cytoplasmic Ca(2+) levels (PubMed:12215548). Activates RALA; this leads to the activation of phospholipase C (PLC) and the formation of inositol 1,4,5-trisphosphate (PubMed:11040035, PubMed:12215548, PubMed:23478264). Signaling mediates activation of down-stream MAP kinases (PubMed:11040035). Contributes to the regulation of cell shape (PubMed:8922387, PubMed:9600933, PubMed:11040035, PubMed:11087877). Promotes Rho-dependent reorganization of the actin cytoskeleton in neuronal cells and neurite retraction (PubMed:9600933, PubMed:11040035, PubMed:12181339). Promotes the activation of Rho and the formation of actin stress fibers (PubMed:9600933, PubMed:12215548). Promotes formation of lamellipodia at the leading edge of migrating cells via activation of RAC1 (PubMed:23478264). Through its function as lysophosphatidic acid receptor, plays a role in chemotaxis and cell migration, including responses to injury and wounding (PubMed:11087877, PubMed:18066075, PubMed:23478264). Plays a role in triggering inflammation in response to bacterial lipopolysaccharide (LPS) via its interaction with CD14 (PubMed:21821728). Promotes cell proliferation in response to lysophosphatidic acid (PubMed:9600933, PubMed:11087877, PubMed:12215548, PubMed:18157949, PubMed:17692995, PubMed:23478264). Required for normal skeleton development (PubMed:21569876). May play a role in osteoblast differentiation (PubMed:21569876). Required for normal brain development (PubMed:17656621, PubMed:18708146). Required for normal proliferation, survival and maturation of newly formed neurons in the adult dentate gyrus (PubMed:18708146). Plays a role in pain perception and in the initiation of neuropathic pain (PubMed:15195086, PubMed:19689455).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 encode the same isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205601 | Lpar1 (tGFP-tagged) - Mouse endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 2 (Edg2) |
CNY 7,088.00 |
|
| MG226281 | Lpar1 (tGFP-tagged) - Mouse lysophosphatidic acid receptor 1 (Lpar1) transcript variant 1, (10ug) |
CNY 7,088.00 |
|
| MR226281 | Lpar1 (Myc-DDK-tagged) - Mouse lysophosphatidic acid receptor 1 (Lpar1), transcript variant 1 |
CNY 5,488.00 |
|
| MR226281L3 | Lenti ORF clone of Lpar1 (Myc-DDK-tagged) - Mouse lysophosphatidic acid receptor 1 (Lpar1), transcript variant 1 |
CNY 4,750.00 |
|
| MR226281L4 | Lenti ORF clone of Lpar1 (mGFP-tagged) - Mouse lysophosphatidic acid receptor 1 (Lpar1), transcript variant 1 |
CNY 4,750.00 |
