Mcoln2 (NM_001005846) Mouse Untagged Clone

CAT#: MC202191

Mcoln2 (untagged) - Mouse mucolipin 2 (Mcoln2), transcript variant 2, (10ug)



  "NM_001005846" in other vectors (4)

CNY 4,016.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-TRPML2
    • 50 ul

CNY 11,000.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 3300002C04Rik; AI549968; C86638; TRPML2
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC029847 sequence for NM_001005846
GAGATCAGAGCGCTGACTGGAAGGATCTGCGATGGCGTCTCTCGATTCTGAGGTGAGGGAAGAGTGTCTG AGGGAAGACCTGAAGTTCTACTTCATGAGTCCCTGTGAGAAGTACCGAGCCAGACGTCAGATCCCGTGGA AACTGGGCTTGCAGATATTGAAGATAGTCATGGTAACCACACAGCTTGTTCGGTTTGGCTTAAGTAACCA GTTGGTGGTGGCTTTCAAAGAAGATAACACTGTTGCTTTTAAGCACTTGTTTTTGAAAGGATTTTCTGGA GTTGATGAAGATGACTACAGCTGTAGCATATACACTCAGGAGAACACCTATGAGAGCATCTTTTTTGCTA TAAAGCAGTATCGTCATCTGAAGAATATTTCCCTGGCAACCCTTGGTTATGGAGAAAGTGAAGACAATAG AACTGGCTTGAAGGTCTGTAAGCAGCACTACAAGACAGGGGCCATGTTTTCTTCTAACGAGACCCTGAAC ATTGACAGTGACATTGAGACAGACTGCATTCACCTGGACCTTCAGGTGCTCACCACGGAACCCGAGGACT GGGCACAGACATCTTTCTTCAGGCTGGATTTTTATCGGCTCGTGCAAGTTGATATCTCCTTTGCCCTCAA AGGAATTGACCTACAGGCAGTTCATTCCCGAGAGATACCGGACTGTTACCTCTTTCAGAATACGATTACC TTCGACAACACAGCCCACAGTGGGAAAATCAAAATCTATCTCAACAGCGAGGCCAACATCGAAGAGTGTA AAAACATGAACATATCTGGGTCTACTCAGAGAAGCACTCACTACCTCCTGGTGTTTGATGTGTTCGTCAT TATGATCTGCCTGGCATCCCTTATCCTGTGCACGAGATCCATTGTTCTTGCTCTGAGGCTGAGGAAGAGA TTTCTAAACTTCTTCCTGGAGAAGTACAAACAGCGCGTGTGTGGCGCTGACCAGTGGGAATTCGTCAACG GATGGTACGTCCTGGTCACTATCAGCGACCTCATGACAATAATTGGATCCATATTGAAGATGGAAATCAA AGCCAAGAAGCTCACAAACTATGACGTGTGCAGCATCTTGCTCGGGACGTCGACGCTCTTTGTCTGGGTT GGAGTCATCAGATATCTGGGTTACTTTCAGACGTACAACGTGCTCATTTTAACCATGCAAGCCTCACTGC CCAAAGTTCTCCGGTTCTGTGCTTGCGCTGGCATGATCTACCTGGGGTACACCTTCTGCGGCTGGATTGT CTTAGGACCGTACCACGAGAAGTTTGAAAACCTGAACATAGTCGCTGAATGCCTGTTTTCTCTGGTCAAC GGCGATGACATGTTTGCCACGTTCGCTCAGATCCAGCAGAAGAGCATTCTGGTGTGGCTGTTCAGCCGCC TCTACCTGTATTCCTTTATCAGCCTATTCATTTACATGGTCCTCAGCCTCTTCATCGCGCTCATCACCGA CTCTTACCACACCATTAAGAAGTACCAGCAGCATGGGTTTCCTGAAACAGATCTGCAGAAATTCCTGAAG GAGAGCGGAAGCAAAGATGGGTACCAGAAGCAGCCATCGGCCTTGCTGTCCTGCCTCTGCTGTCTGAGGA GGAGAAGAAACAATGACCACTTGATTCTCATTGACTAAAACCTTTGGCTGATGTCAGTCAAGTCCAGATC CCTTTATGCTGAGGTCATTCAGAGAGACCCCAGAGGTAGCAGCAGTCGGCTCAAAGATTGTCCTGCTGAA TTGTGGAACCAGAAGGAGGGGTGTCCATGAGCTGCTGATCAGACCTGACATTCCAAAGTTACTTTTCATA GACATGGAGTGTGAAGAGTAAGCCTCTTGTGAGGCCAGCGTGGCAATGAAATTCACCAGGAGTCTGGTCG TTTGTGACATCATAATGTTGAGATGTAAAAAGAAATATTCCCACTTCAGAGTGTGTAAGCAATATTTAAA CCCAGATAAAGATGCATGCTTATTGAAAAAACAAAGATTTGTTTCAAAACGTGACACTTGGATTTGAAGA CATTTGTTAATTTATGGTCTGCACAGAAATAAATTTGCTTCCAAACCAAATTAAGACTTGAAGCTAGAGA GTGGTAAGCCACTCAGTTCGTGACTGGTTGGACTGTGAGCTTGCTTCTTCAGCAAGCACAACACCAGTGT CTGTTAGCACAGTCAGCGGGATGAAAGAGCACCTGGCTGTCCCCTCAGGTGAGCTCTCCCTGGACTGGAC ACATGATGAGTGAGTCACCCTAAGCTTGGAGTGGCCATCTGTTTCACAAGTCAGCTCCATTGTGGTGGGG ATTTGCCCTTGTCATGGAAGGAAGTGTAGATTTTTCATTAAATAAATTTATTTTCACGAAAAAAAAAAAA AAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_001005846
Insert Size 1617 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC029847, AAH29847
RefSeq Size 2390 bp
RefSeq ORF 1617 bp
Locus ID 68279
UniProt ID Q8K595
Gene Summary Nonselective cation channel probably playing a role in the regulation of membrane trafficking events. Acts as Ca(2+)-permeable cation channel with inwardly rectifying activity (PubMed:19763610). May activate ARF6 and be involved in the trafficking of GPI-anchored cargo proteins to the cell surface via the ARF6-regulated recycling pathway (By similarity). May play a role in immune processes. In adaptive immunity, TRPML2 and TRPML1 may play redundant roles in the function of the specialized lysosomes of B cells (PubMed:17050035). In the innate immune response, may play a role in the regulation of chemokine secretion and macrophage migration (PubMed:26432893). Through a possible and probably tissue-specific heteromerization with MCOLN1 may be at least in part involved in many lysosome-dependent cellular events.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) has an alternate 5' exon and uses a downstream AUG start codon, as compared to variant 1. The encoded isoform 2 has a shorter N-terminus, as compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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