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Cyp1a2 (NM_009993) Mouse Untagged Clone
CAT＃: MC202251
Cyp1a2 (untagged) - Mouse cytochrome P450, family 1, subfamily a, polypeptide 2 (Cyp1a2), (10ug)
CNY 3,832.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | CP12; Cyp1a1; CYPIA2; P450-3 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC054827 sequence for NM_009993
ACTGACTCCCACAACTCTGCCAGTCTCCAGCCCCTGCCCTTCAGTGGTACAGATGGCGTTCTCCCAGTAC ATCTCCTTAGCCCCAGAGCTGCTACTGGCCACTGCCATCTTCTGTTTAGTGTTCTGGATGGTCAGAGCCT CAAGGACCCAGGTTCCCAAAGGCCTGAAGAATCCACCCGGACCCTGGGGCTTGCCCTTCATTGGGCACAT GCTGACTGTGGGGAAGAACCCACACCTGTCACTGACACGGCTGAGTCAGCAGTATGGGGACGTGCTGCAG ATCCGCATCGGCTCCACTCCTGTGGTGGTGCTGAGCGGCCTGAACACCATCAAGCAGGCCCTGGTGAGGC AGGGAGATGACTTCAAGGGCCGACCAGACCTCTACAGCTTCACACTTATCACTAACGGCAAGAGCATGAC TTTCAACCCAGACTCTGGACCCGTGTGGGCTGCCCGCCGGCGCCTGGCCCAGGATGCCCTGAAGAGCTTC TCCATAGCCTCGGACCCGACGTCAGCATCCTCTTGCTATTTGGAGGAGCACGTGAGCAAGGAGGCTAACC ATCTCGTCAGCAAGCTTCAGAAGGCGATGGCAGAGGTTGGCCACTTCGAACCAGTCAGCCAGGTGGTGGA ATCGGTGGCTAACGTCATTGGTGCCATGTGCTTTGGGAAGAACTTCCCCCGGAAGAGCGAGGAGATGCTG AACATCGTGAATAACAGCAAGGACTTTGTGGAGAATGTCACCTCAGGGAATGCAGTGGACTTCTTCCCGG TCCTGCGCTACCTGCCCAACCCGGCCCTCAAGAGGTTTAAGACCTTCAATGATAACTTCGTGCTGTTTCT GCAGAAAACTGTCCAGGAGCACTACCAAGACTTCAACAAGAACAGTATCCAAGACATCACAAGTGCCCTG TTCAAGCACAGCGAGAACTACAAAGACAATGGCGGTCTCATCCCCGAGGAGAAGATTGTCAACATTGTCA ATGACATCTTTGGAGCTGGCTTTGACACAGTCACCACAGCCATCACCTGGAGCATTTTGCTACTTGTGAC ATGGCCTAACGTGCAGAGGAAGATCCATGAGGAGCTGGACACGGTGGTTGGCAGGGATCGGCAACCACGG CTTTCTGACCGTCCCCAGCTGCCATATCTAGAGGCCTTCATCCTGGAGATCTACCGATACACATCCTTTG TCCCCTTCACCATCCCCCACAGCACAACGAGGGACACCTCACTGAATGGCTTCCACATTCCCAAGGAGCG CTGTATCTACATAAACCAGTGGCAGGTCAACCATGATGAGAAGCAGTGGAAAGACCCCTTTGTGTTCCGC CCAGAGCGGTTTCTTACCAATAACAACTCGGCCATCGACAAGACCCAGAGCGAGAAGGTGATGCTCTTCG GCTTGGGAAAGCGCCGGTGCATTGGGGAGATCCCGGCCAAGTGGGAAGTCTTCCTCTTCTTAGCCATCCT GCTGCAGCATCTGGAGTTTAGTGTGCCACCGGGTGTGAAGGTGGACCTGACACCCAACTATGGGTTGACC ATGAAGCCCGGGACCTGTGAACACGTCCAGGCATGGCCACGCTTTTCCAAGTGAAGATTGTCGAGGCATC GGTGGGGCCGTCACCCTTGTTTCTTTTCCTGTTTTAAAAAAAAAAAAAAAAACAGCTTTTTTTTTTTTGA GAGATACAATTCTTTCCCCATTTAATTCATCTCCAAGCAATTTTACAATAGTGTCTATCATGTTCACCCC ATAACCCATACTCATTAGGACTTATGATTTAAGATTCCTCCTACCCTGTCTTGCTTGCCGCACCTCATGC TAATCTAGTTTTTGACTCAATAGATTTGCCTACTCTGGCTGTCTCATATAAATCGAATGAATTATAAAAA AAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_009993 |
| Insert Size | 1542 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC054827, AAH54827 |
| RefSeq Size | 1900 bp |
| RefSeq ORF | 1542 bp |
| Locus ID | 13077 |
| UniProt ID | P00186 |
| Gene Summary | A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer. Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. Metabolizes caffeine via N3-demethylation.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208242 | Cyp1a2 (tGFP-tagged) - Mouse cytochrome P450, family 1, subfamily a, polypeptide 2 (Cyp1a2) |
CNY 4,940.00 |
|
| MR208242 | Cyp1a2 (Myc-DDK-tagged) - Mouse cytochrome P450, family 1, subfamily a, polypeptide 2 (Cyp1a2) |
CNY 3,832.00 |
|
| MR208242L3 | Lenti ORF clone of Cyp1a2 (Myc-DDK-tagged) - Mouse cytochrome P450, family 1, subfamily a, polypeptide 2 (Cyp1a2) |
CNY 6,370.00 |
|
| MR208242L4 | Lenti ORF clone of Cyp1a2 (mGFP-tagged) - Mouse cytochrome P450, family 1, subfamily a, polypeptide 2 (Cyp1a2) |
CNY 6,370.00 |
