Col3a1 (NM_009930) Mouse Untagged Clone

CAT#: MC202519

Col3a1 (untagged) - Mouse collagen, type III, alpha 1 (Col3a1), (10ug)



  "NM_009930" in other vectors (2)

CNY 10,024.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AW550625; Col3; Col3a-1; Tsk; Tsk-; Tsk-2; Tsk2
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC058724 sequence for NM_009930
ACCATTCTGATGTTGGAGCGGCCGCGTGCTGAAGGGCAGGGACAACTGATGGTGCTACTCTGAGCTGCTT CTTCCTCTCTCCTCTTTTGCACAAAGAGTCTCATGTCTGATATTTAGACATGATGAGCTTTGTGCAAAGT GGAACCTGGTTTCTTCTCACCCTTCTTCATCCCACTCTTATTTTGGCACAGCAGTCCAACGTAGATGAAT TGGGATGCAGCCACCTTGGTCAGTCCTATGAGTCTAGAGATGTCTGGAAGCCAGAACCATGTCAAATATG TGTCTGTGACTCAGGATCTGTCCTTTGCGATGACATAATCTGTGATGAGGAGCCACTAGACTGCCCCAAC CCAGAGATCCCATTTGGAGAATGTTGTGCAATATGCCCACAGCCTTCTACACCTGCTCCTGTGCTTCCTG ATGGCCATGGACCTCAAGGCCCCAAGGGAGATCCGGGTCCTCCTGGCATTCCTGGGAGAAATGGTGACCC TGGCCTTCCAGGACAACCAGGTCTCCCTGGGCCTCCTGGCTCCCCTGGAATCTGTGAATCATGTCCAACT GGTGGCCAAAATTATTCTCCCCAATTCGACTCATATGATGTCAAGTCTGGAGTGGGAGGAATGGGTGGCT ATCCGGGACCAGCTGGTCCACCAGGCCCTCCAGGACCCCCTGGTTCTTCTGGACATCCTGGATCTCCTGG TTCTCCTGGATACCAAGGTCCCCCTGGTGAACCTGGTCAAGCTGGTCCGGCAGGCCCTCCAGGACCTCCT GGTGCTCTTGGTCCAGCTGGTCCTGCTGGAAAGGATGGAGAGTCAGGAAGACCTGGACGACCTGGAGAGC GTGGACTGCCTGGACCTCCAGGTATCAAAGGCCCAGCTGGCATGCCTGGATTCCCTGGTATGAAAGGACA CAGAGGCTTTGATGGACGCAATGGAGAAAAGGGAGAAACAGGTGCTCCTGGACTGAAGGGTGAAAATGGT CTTCCAGGAGACAACGGAGCTCCTGGCCCCATGGGTCCTAGAGGGGCTCCTGGTGAGCGAGGACGACCAG GCCTTCCTGGAGCTGCAGGTGCTCGAGGCAATGATGGTGCTCGGGGCAGTGATGGGCAACCTGGTCCCCC TGGCCCTCCTGGAACTGCAGGATTCCCTGGATCCCCTGGTGCTAAGGGTGAAGTTGGACCTGCAGGGTCC CCTGGCTCAAATGGCTCACCAGGACAAAGAGGGGAGCCTGGACCGCAGGGACATGCTGGTGCTCAAGGGC CTCCTGGCCCTCCCGGGAATAACGGCAGTCCTGGTGGAAAGGGTGAAATGGGTCCAGCTGGCATTCCTGG AGCCCCTGGACTAATAGGAGCAAGGGGCCCCCCTGGACCAGCAGGAACTAATGGTATACCCGGAACACGA GGTCCTTCAGGTGAACCCGGCAAGAACGGTGCGAAGGGAGAGCCAGGAGCACGCGGTGAACGGGGCGAAG CTGGTTCCCCAGGAATTCCAGGACCTAAGGGCGAAGATGGCAAAGATGGATCACCTGGAGAACCTGGTGC AAATGGGCTTCCAGGAGCCGCAGGAGAAAGGGGTCCTAGTGGCTTCCGAGGACCTGCAGGGCCAAATGGC ATCCCAGGAGAAAAGGGTCCTCCCGGAGAGCGTGGTGGCCCAGGTCCTGCAGGACCCAGAGGAGTAGCTG GAGAACCTGGCCGAGATGGAACCCCTGGAGGTCCAGGGATACGGGGTATGCCCGGAAGTCCAGGTGGACC AGGCAATGATGGAAAACCAGGACCTCCTGGAAGTCAAGGAGAAAGTGGTCGCCCTGGTCCCCCTGGCCCA TCTGGTCCCAGAGGTCAGCCTGGTGTCATGGGTTTCCCTGGTCCTAAAGGAAATGATGGTGCCCCTGGCA AGAATGGAGAACGGGGTGGCCCTGGAGGACCTGGCCTTCCAGGTCCTGCTGGAAAGAATGGGGAGACTGG ACCTCAGGGTCCCCCAGGACCTACTGGCCCAGCTGGTGACAAGGGAGATTCTGGACCCCCTGGTCCACAA GGATTACAAGGCATACCTGGTACCGGTGGTCCTCCAGGAGAAAATGGGAAACCAGGTGAACCAGGTCCAA AGGGTGAAGTCGGTGCTCCTGGAGCTCCTGGAGGCAAGGGTGATTCTGGTGCACCTGGAGAACGTGGGCC TCCTGGAACAGCAGGAATCCCTGGTGCTAGAGGTGGAGCTGGACCCCCTGGCCCTGAGGGAGGAAAGGGC CCTGCTGGTCCCCCTGGCCCTCCTGGTGCTTCTGGTTCTCCTGGTCTGCAAGGGATGCCAGGGGAAAGAG GAGGTCCTGGAAGTCCTGGCCCAAAGGGTGAAAAGGGTGAACCAGGTGGTGCAGGTGCTGACGGAGTTCC AGGAAAGGATGGGCCAAGGGGTCCTGCTGGCCCTATTGGTCCCCCTGGCCCAGCTGGTCAGCCTGGAGAT AAGGGTGAAGGTGGTTCCCCTGGACTTCCAGGTATAGCTGGACCTCGAGGTGGCCCTGGTGAGAGAGGTG AACATGGCCCTCCAGGACCTGCTGGCTTCCCGGGTGCTCCTGGACAGAATGGTGAACCAGGTGCTAAAGG AGAAAGAGGTGCCCCTGGAGAGAAAGGTGAAGGAGGCCCTCCAGGACCTGCAGGACCCACTGGCAGTTCT GGACCTGCTGGTCCGCCTGGTCCTCAGGGTGTAAAGGGTGAACGTGGTAGTCCTGGTGGTCCTGGTACTG CTGGCTTTCCCGGTGGGCGTGGTCTTCCTGGTCCTCCTGGCAATAATGGTAATCCAGGGCCCCCAGGACC CAGTGGTGCTCCTGGCAAGGACGGCCCTCCAGGTCCTGCAGGCAACAGTGGTTCTCCTGGCAACCCTGGA ATAGCTGGACCAAAAGGTGATGCTGGACAGCCTGGAGAGAAGGGGCCACCTGGTGCTCAGGGTCCTCCGG GTTCCCCAGGCCCACTTGGAATTGCAGGGCTAACTGGAGCACGAGGTCTTGCTGGACCACCAGGCATGCC AGGTCCACGGGGTAGCCCTGGACCTCAGGGTATCAAGGGTGAAAGTGGAAAACCAGGAGCCAGTGGCCAT AATGGGGAACGTGGTCCTCCTGGACCCCAAGGTCTTCCTGGTCAGCCTGGTACAGCTGGTGAACCTGGAA GGGATGGAAACCCTGGATCAGATGGTCAGCCAGGTCGAGATGGATCTCCTGGTGGCAAGGGTGATCGTGG TGAAAATGGTTCTCCTGGTGCCCCAGGCGCTCCTGGTCATCCAGGCCCACCTGGCCCTGTTGGTCCATCT GGAAAGAGTGGTGACAGAGGAGAAACTGGCCCTGCTGGTCCTTCTGGTGCTCCAGGCCCTGCTGGAGCTC GAGGTGCTCCGGGTCCCCAAGGTCCACGAGGTGACAAAGGTGAAACTGGTGAACGTGGCTCTAATGGCAT CAAAGGACATCGAGGATTCCCTGGCAATCCAGGTCCTCCAGGTTCTCCTGGTGCTGCTGGTCACCAGGGT GCAATTGGTAGTCCAGGACCTGCAGGTCCCAGAGGACCAGTTGGACCACATGGACCTCCTGGAAAAGATG GAACAAGTGGGCATCCAGGTCCTATTGGACCACCAGGTCCTAGAGGAAACAGAGGTGAAAGAGGATCTGA GGGCTCGCCAGGCCACCCTGGACAGCCAGGACCCCCTGGACCCCCTGGTGCCCCTGGTCCCTGCTGTGGT GGTGGTGCTGCTGCCATTGCTGGAGTTGGAGGTGAAAAGTCTGGTGGCTTTTCACCCTATTATGGAGACG ATCCAATGGATTTCAAGATCAACACTGAAGAGATTATGTCTTCACTCAAGTCTGTTAATGGACAAATAGA GAGTCTTATAAGCCCTGATGGTTCTCGAAAAAACCCTGCTCGGAACTGCAGAGACCTAAAATTCTGCCAC CCCGAACTCAAGAGTGGAGAATACTGGGTTGATCCTAACCAAGGCTGCAAGATGGATGCTATAAAAGTAT TCTGTAACATGGAAACTGGGGAAACATGCATAAATGCCAGCCCCATGACTGTCCCACGTAAGCACTGGTG GACAGATTCTGGTGCAGAGAAGAAACATGTTTGGTTTGGAGAATCTATGAATGGTGGTTTTCAGTTCAGC TATGGCCCTCCTGATCTTCCTGAAGATGTCGTTGATGTGCAGCTGGCATTCCTCAGACTTCTTTCCAGCC GGGCCTCCCAGAACATTACATACCACTGCAAAAACAGTATTGCCTATATGGATCAGGCCAGTGGCAATGT AAAGAAGTCTCTGAAGCTGATGGGATCAAATGAAGGCGAATTCAAGGCTGAAGGAAACAGCAAATTCACT TACACAGTTCTAGAGGATGGCTGTACTAAACACACTGGGGAATGGAGCAAGACAGTCTTTGAATATCAAA CACGCAAGGCAATGAGACTACCCATCATAGATATCGCACCCTATGACATTGGGGGTCCTGATCAAGAATT TGGTGTGGACATTGGCCCTGTTTGCTTTTTATAAGCCAAACTCTCTGAAACCCCAGCAAAACAAAAACCA CATCCATGTGTTCATCTTGTTTTAATCTTATCAACCAGTGCAAGTGACCAACTAAATTCCAGTTATTTAT TTCCAAACTTTTGGAAAAAGCATAATTTGACAAAAAAAGAATACAATTTTTTGCTGTTTCAACCACCCAA TACAGGTCAAATGCTTTTGTTTTATTTTTTTACCAATTCCAACTTCAAAATGTCTCAATGGTGCTATAAT AAATAAACGTCAACACTTTTATGATAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI     
ACCN NM_009930
Insert Size 4395 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC058724, AAH58724
RefSeq Size 4812 bp
RefSeq ORF 4395 bp
Locus ID 12825
UniProt ID P08121
Gene Summary This gene encodes the alpha-1 subunit of the fibril-forming type III collagen found in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. The encoded protein forms homotrimeric type III procollagen that undergoes proteolytic processing during fibril formation. A majority of mice lacking the encoded protein die within two days of birth but about 5% of the animals survive to adulthood. The surviving mice exhibit severe cortical malformation and experience significantly shorter lifespan. The mutant mouse named "tight skin 2" exhibiting systemic sclerosis phenotype was found to harbor a missense point mutation in this gene. A pseudogene of this gene has been defined on chromosome 8. [provided by RefSeq, Nov 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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