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Ctcf (NM_007794) Mouse Untagged Clone
CAT＃: MC202667
Ctcf (untagged) - Mouse CCCTC-binding factor (Ctcf), (10ug)
CNY 8,840.00
货期*
现货
规格
| Cited in 1 publication. |
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AW108038 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC049131 sequence for NM_007794
AAACCGTGCGCGGAGCTGCTTCTTTGGCGGCAGCGGCGACCAGCAGCAGCCGGTGCGGGCGCGCGGAGCC GGCCGGGGACGCCAGGTGGCCGGAGCGGTGGAGCGGCGGCGGAGCGGGCGCCGCGGGGGGTGTGGCGCGA AGAATGACCACAAATCTAGAACCAAAGAACACGATGCACTAGCAGACAGACTCGAAGCTAGAGACTGGAG ATCTGGGTTCTTTTGTCCTTCCTCAAACTGATTTTGCAGCCACGGAGAGGAAGGGGAGATGGAAGGTGAG GCGGTTGAAGCCATTGTGGAGGAGTCTGAAACTTTCATTAAAGGAAAAGAAAGAAAGACTTACCAGAGAC GCCGGGAAGGGGGCCAGGAAGAAGATGCTTGCCACCTGCCCCAGAACCAGACAGATGGGGGTGAGGTGGT CCAGGATGTCAACAGCAGTGTACAGATGGTAATGATGGAACAGCTGGATCCTACCCTTCTCCAGATGAAG ACTGAAGTCATGGAGGGTACAGTGGCTCCGGAAGCAGAGGCTGCAGTGGACGATACCCAGATCATAACCT TGCAGGTTGTAAATATGGAGGAACAGCCCATTAACATAGGAGAGCTTCAGCTTGTCCAAGTACCTGTTCC TGTGACGGTACCTGTTGCTACTACTTCAGTAGAAGAACTTCAGGGGGCTTATGAGAATGAAGTGTCTAAA GAGGGCCTTGCAGAAAGTGAACCGATGATATGTCACACCTTACCTTTGCCTGAAGGATTTCAGGTGGTGA AAGTGGGGGCCAATGGAGAAGTGGAGACACTAGAGCAGGGCGAGCTTCCTCCTCAGGAAGACTCTAGCTG GCAAAAAGACCCAGACTATCAGCCACCAGCCAAAAAAACAAAGAAAACCAAAAAGAGCAAACTTCGTTAC ACAGAAGAGGGCAAAGACGTGGATGTGTCTGTGTATGATTTTGAGGAAGAACAGCAGGAAGGACTGCTGT CTGAGGTTAATGCAGAGAAAGTAGTTGGTAATATGAAGCCTCCGAAGCCAACAAAAATTAAAAAAAAAGG TGTAAAGAAAACATTCCAGTGTGAGCTTTGCAGTTACACATGTCCCCGGCGTTCAAATTTGGATCGTCAC ATGAAAAGCCACACTGATGAGAGACCACACAAATGCCATCTGTGTGGCAGAGCATTCAGAACAGTGACCC TCCTGAGGAATCATCTGAACACACACACAGGTACTCGTCCTCACAAGTGCCCAGACTGCGATATGGCCTT TGTGACCAGTGGAGAATTGGTGCGGCATCGTCGTTATAAACACACTCATGAGAAACCATTTAAGTGTTCC ATGTGTGATTATGCCAGTGTAGAAGTCAGCAAATTAAAACGACACATTCGCTCTCATACTGGAGAGCGCC CGTTCCAGTGCAGTTTGTGCAGTTATGCCAGCAGGGACACATACAAGCTGAAAAGGCATATGAGAACCCA TTCAGGGGAAAAACCTTATGAATGTTATATTTGTCACGCTCGGTTTACCCAGAGTGGTACCATGAAGATG CACATTTTACAGAAGCACACAGAAAATGTGGCCAAATTTCATTGTCCCCATTGTGACACTGTCATAGCCC GAAAAAGTGATTTGGGTGTCCACTTGCGAAAGCAGCATTCCTATATTGAACAGGGCAAAAAATGTCGCTA CTGTGATGCTGTGTTTCATGAGCGATATGCTCTCATCCAGCATCAAAAATCACACAAAAATGAGAAGCGC TTCAAGTGTGACCAGTGTGATTATGCTTGTAGACAGGAGCGGCACATGATCATGCACAAGCGCACTCACA CGGGGGAGAAGCCTTATGCCTGCAGCCACTGCGACAAGACCTTCCGCCAGAAACAGCTCCTCGACATGCA TTTCAAGCGCTATCATGATCCCAACTTTGTCCCTGCTGCCTTTGTCTGTTCCAAGTGTGGGAAAACATTC ACCCGCCGGAACACAATGGCAAGACATGCAGATAACTGTGCTGGTCCAGATGGCGTAGAGGGGGAAAATG GAGGGGAGACAAAGAAGAGCAAACGAGGAAGAAAAAGAAAGATGCGATCTAAAAAAGAAGACTCCTCTGA CAGTGAAGAAAATGCTGAGCCGGATCTCGATGACAATGAGGAGGAGGAGGAGCCTGCTGTAGAAATTGAA CCTGAGCCAGAGCCTCAGCCCCAGCCTCCGCCTCCACCTCAGCCTGTGGCCCCGGCCCCCCCACCTGCCA AGAAGAGAAGGGGGCGACCTCCTGGAAGAACCAACCAGCCCAAACAGAACCAGCCAACAGCCATCATTCA GGTCGAAGATCAGAATACAGGTGCAATTGAGAACATTATAGTTGAAGTCAAAAAGGAGCCAGATGCCGAG CCTGCGGAGGGGGAAGAAGAGGAGGCTCAGGCAGCCACCACAGACGCCCCCAACGGAGACCTCACGCCTG AGATGATCCTCAGCATGATGGACCGGTGATGCTGGGGCCTTGCTCGGCACCAGGACTATTGGGCTGTGTT TAAACGGCCCAAATCTTAATTTTTCTCTTTTTTTTCTTTGGCTTTGGGAACGGCATAATTTTACACCATT TTACCAAACATACTGAGAACGAAAACTTCAAGGATGATGTTAGAAAAATGTGATTTAACTAGAACTTGTT TGATGTTAGCAAATCATGGAATGTTCTAAGTCTCTGAGGGTTTACTGTGAAGTGTTGAGGACAGTGTTGA TGCCTAACTAGTTTTCTTAGATGGAAACAGAGACATTGAGCCCTCTCTCTTGATCGTAAACCACTCCAGA ACGGCCACGGGTTTCCCAGAGTTCTATGGTCTTCCCAAGAGAATTTTTAATTGTAAATGCAGACTTGGGA AGGACTTGGACTTTTAACCTGGTTTTGCTTTTGCTTTTCCTGACTCCCTTTGCTCAGAGTCAGTTGCACA CCAGTAGGATGGCATGCTACGATCAGTTTCTGTCCTGAAAGCTTTGCCTCTTTCTTGGCAAAGTTTCTGG TATGGTCAAGCTTGTAAATAACCTTTTTTACATTTTAATCTTTTCCATTAATTAAGAGGTTGAAAAGAAG TACAGTGTAAGAAAACCCAGCATTTTAATTATTTGCAAATTAAGGTGCCACAGACACAGACTTAGTGGTA TGTAAATGTTGACTCGGATCACAATCATGTAGCAGAGGGCACTGGGCCTGTTGCCTGCCAGTCATGGACA CCCATGTGAAAAATCCTGATTTCCAGCTCATGGAGCCAGCGTTTGAACCTTGAACAAATAAATTTCATTT ATGATTTTCTCATCAACATTTTCTTTGCTCTGTTTGTAGCTGAATGTTACATTTTTTTAAAAATCTCTGT TCAAGCCAGAAGAGTTGATTATGAGTGGCCACAGCAACCCTGGCAAGCTGGGCCAAAAAATTTCAGTAGG TCAGTAATTTAAACTTCACATCTGGAAAAAAAATTATTAATAATGTGAAACAAACCAACTTAAAAAGTGA TTCTTGCACATGAATTGTCACATATTATATGTGTTTTTTAAAGAGCCTCAGTCTGACTGATTTCAAACAC TTTTTTTCTCCTGTGTATTGCTTTTAAGAGAGCCATCAGTTAGCTATCAGAATCTAGGTTGATGCATTTT TGTACTTAGCTGTACTGTGTGATATTTTTCATTATTTTAGGATGCCAACATGAGACCTGTAATAAAATAT GTAATGGGGTTGAAGGCTGGGGAGGAGGATCTACTGCTGTACAGCTAATAAATCATAACGGATTAACAGG CAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | NM_007794 |
| Insert Size | 3807 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC049131, AAH49131 |
| RefSeq Size | 3807 bp |
| RefSeq ORF | 3807 bp |
| Locus ID | 13018 |
| Gene Summary | Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays an important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping (By similarity). Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X-inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19. Plays a role in the recruitment of CENPE to the pericentromeric/centromeric regions of the chromosome during mitosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Trim33 is required for appropriate development of pre-cardiogenic mesoderm
,Rajderkar, S;Mann, JM;Panaretos, C;Yumoto, K;Li, HD;Mishina, Y;Ralston, B;Kaartinen, V;,
Dev. Biol.
,PubMed ID 30940539
[CTCF]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210366 | Ctcf (tGFP-tagged) - Mouse CCCTC-binding factor (Ctcf) |
CNY 6,370.00 |
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