Sgpp1 (NM_030750) Mouse Untagged Clone

CAT#: MC203228

Sgpp1 (untagged) - Mouse sphingosine-1-phosphate phosphatase 1 (Sgpp1), (10ug)



  "NM_030750" in other vectors (4)

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI463453; SPP; SPP1; Spph1
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC037592
CCACGCGTCCGCGGACGCGTGGGGTCGCCGGCAGCCTCGAGCTAGTCGAGCTAGGCACCGTGGCGGCTGT GGCTGGCGAGGAGCGCGTGGCCGGATCAATTCCCGAGTGGCCCGCGACCATGTCCCTGGGGCAGCGGCTG GCTCTGCTGGCCAGCCGTCTGCAGGAGCCGCAGCGGGTGGCGAGCTTCCAGCGTCTGTGTGGGGTGGAGG TGCCGCTCAGCAGCCCGGCGGCGGACGAGGATGCAGAGACCGAGGTTCGCGGAGCCCCGGGAGAACCCCG GCGACGGGGACGGCAGCCGGGCGCTGAGGACAGCCCCGCCAAGGCGGACTGCTGCGGTGCCCCGAACGGC GTGCGCAACGGGCTGGCGGCCGAGCCGGGCCCGACCGGGCCCCGCCGCGCGGGCTCGCAGCGCCGCAACT CGTTGACGGGCGAGGAGGGCGAGCTGGTCAAAGTGAGCAACTTGCCGCTCTACTACCTGTTCTGCCTAGG CACGGAACTGGGCAACGAGCTCTTCTACATCTTATTCTTCCCCTTCTGGATCTGGAATCTCGACCCCTTT GTGGGCCGGAGGCTGGTGATCATCTGGGTGCTGGTCATGTACCTGGGCCAGTGCACCAAGGACATCATCC GCTGGCCACGGCCGGCCTCGCCGCCTGTCATCAAGCTGGAGGTCTTCTACAACTCGGAATACAGCATGCC CTCCACGCATGCCATGTCAGGCACCGCCATCCCCATCGCCATGTTCCTGCTCACCTATGGCCGCTGGCAG TATCCTCTTATCTACGGGCTGATTCTCATTCCCTGCTGGAGTTCACTAGTTTGCCTAAGTAGAATCTACA TGGGAATGCATTCTATCCTGGATGTCATTGCTGGATTCTTGTATACCATTTTAATCTTAATTATCTTCTA CCCATTGGTGGACCTGATTGACAACTTCAACCAAACTTACAAATATGCGCCGCTCATCATCATCGGGCTT CACTTAATTTTGGGCATCTTCTCTTTCACCCTTGACACCTGGAGCACATCCCGAGGAGACACGGCTGAGA TTCTGGGAAGTGGTGCTGGGATTGCATGTGGCTCACACGCTGCTTATACCCTGGGCCTATCCTTAGAACC TTCTCTGCACATGTTACCCTTAGCTATCCCCCCTCTTACTGTAACTCTGTTTGGAAAAGCCATATTACGG ATCGTCCTAGGAATGCTGCTTGTACTGTTCGTGAGGGATATCATGAAGAAGATCACCATTCCTCTAGCCT GTAAACTCTCCAGTATTCCGTGTCATGACATTCGCCAAGCAAGGCAGCACATGGAAGTGGAGCTGCCATA CCGGTATATTACCTACGGGATGGTTGGGTTCTCCATCACGTTTTTGGTCCCCTATGTATTTTCCTTTATT GGTATCTCTTGATGGAGGAACACTGTTTGTTATAAGAAAGGAGGCTACCAGCTATATCTAAAGCTATTCT CTAGGTAAAACTTGGATCAGAGGCTTCTGCAAGAATTTGACTTAAAGAAGTAAATTCTGCAGCCAGTGCA TTCTCTCATTGCACACCAGATGTTGTTTTACGTGGGCTGAGCTCTCTCAGTGCTGAGAAATGGCGCGCCC ACTTAGAATGTTCACCAAATGTTTGGGGAGTTCTGTGCTGTTACAAATTGTAGTTATATATACCATATAT TAAGGCACACGGTGTGCAAAGGTGTGTCTAGTATATATTATATATACAACTGTTTACCTAACAACAGTGG GGTGTATTGAAAAAAATCAGTAACAATATGCAGTTGTGCCCAGGTTTTTGGAATTAATGCAGGCATGTTG TTATTAACAAATAGGCTTAGTGTTTCTTTCCTCTGGGAGCCATTTCCTAGTTGAGTTGGTAATTAGCAGA CAGAAATCTGTGTGCTACCGCACACTTGACTTACTGTCTCTCCCCACACTGCCAGCTCCCTTCATTTACC CATGAACGAGAGGCATTTCTAATGGACCAAGTTTTGGGGGAAGTTTGCTGTTTGAGATTTTTTTTTCCAT TCTTAAAGTTCTGTACACTGTGTTGAATGTATTTTTTTTCCCAACTGTTTTGGTCACACCAATTCAGGTA CTGAATTTTATTGCTTGCTAACTGTGCCTTTCTTTTCCTGAATTAAGAGACACCATGTATACTGTGATAT AAAAGTGACACTCTAATTCTATCATAAGCCACATCATCAGGCAAATATTTTTAAAATATATATATCAAAC TAACAGAAGTAAATGGCCCATAACTCTTCAGTCGTTTTTTAGAAAGAAATGTGGAATTGCTAAGTTTTCT CACTAATTTATTGGAAAGCTAAATGGATAAGTATTTCCATTTTTGTAAGTATTTTTCTGTGCATTGCAAG TTATTGGAACAGTATATATATGTAAGTACGTTGTCTTGATTGTGTATAGTACAAATAAATACTTCCAGGG AGTGGAAACATCACTGAAATTTATAGATGAGACAGTGTATACCCTTCCTATGTACACAAGCCATTTTTCT AAATTATTTGAAAAGGATTGGATTTCCTACCGTTATCTTTTAATACCCCACTCTTTAGCAATCTAAGGTA GATTATAAAATCCAAACGTGAAATATCCGTAGCTAAGATTCTATTATTTTTCCCAACTAATTATTTTTAC AGTGGTAAATTTGAGTGTAGTGTTAAGCTATTCTTTCCTGTCTGTGCCTTTATATTTTGAAGTGTGATTG CAATTAGGTTAACATTACGTTTATTTGTGGTGAGGTTTTTTGTAAAGAAGAGAATTAATTTTCCATACAA ATGAACACCAGGTGGAGGTAATGATTTTAGTGCAATAATCTCTGATTATTTAGAAGGAAAACGTTTCTAT TTTGGACAAGGAAAGTATCTGAGCTTCTTATTTTTTATAATTCTCATTGAAAGCTTGGGGGCTTGGTAGT TTTTACCCACAAAGAATCTGATCAACTTTAAGCAAGTAAATTTGGACCCTTTGGCCACCAGGAGAAGAAA GCGAGGTGCTTAGAAAAGAATCGGTACGCAGGGAGCTCAGAAGCTGCGGAGAGCAGGGATTGCTCCACAG ATGTGCTCTGTGAGTTGAGCTATAGACAGGGTCCCCCACTTTGGGTCTGTTATGCTATTAATTTTTGTGT GTCACTTAAAAAGAATTAGAAAAGATTCTATTTCTCTGTGTTTGTTTTTGTTTGATGACAGTTCTATGCT AAATGTATTTTAAAATAAAAGCCGAGATCAATACCGAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_030750
Insert Size 1293 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC037592, AAH37592
RefSeq Size 3274 bp
RefSeq ORF 1293 bp
Locus ID 81535
UniProt ID Q9JI99
Gene Summary Specifically dephosphorylates sphingosine 1-phosphate (S1P), dihydro-S1P, and phyto-S1P (PubMed:10859351, PubMed:11756451). Does not act on ceramide 1-phosphate, lysophosphatidic acid or phosphatidic acid. Sphingosine-1-phosphate phosphatase activity is needed for efficient recycling of sphingosine into the sphingolipid synthesis pathway. Regulates the intracellular levels of the bioactive sphingolipid metabolite S1P that regulates diverse biological processes acting both as an extracellular receptor ligand or as an intracellular second messenger (PubMed:10859351, Ref.2). Involved in efficient ceramide synthesis from exogenous sphingoid bases. Converts S1P to sphingosine, which is readily metabolized to ceramide via ceramide synthase (PubMed:12235122, PubMed:17895250). In concert with sphingosine kinase 2 (SphK2), recycles sphingosine into ceramide through a phosphorylation/dephosphorylation cycle (PubMed:17895250). Regulates endoplasmic-to-Golgi trafficking of ceramides, resulting in the regulation of ceramide levels in the endoplasmic reticulum, preferentially long-chain ceramide species, and influences the anterograde membrane transport of both ceramide and proteins from the endoplasmic retiulum to the Golgi apparatus (By similarity). The modulation of intracellular ceramide levels in turn regulates apoptosis (PubMed:12235122). Via S1P levels, modulates resting tone, intracellular Ca(2+) and myogenic vasoconstriction in resistance arteries. Also involved in unfolded protein response (UPR) and ER stress-induced autophagy via regulation of intracellular S1P levels (By similarity). Involved in the regulation of epidermal homeostasis and keratinocyte differentiation (PubMed:23637227).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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