Nucb2 (NM_016773) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI607786; Calnuc; Nefa; nesfatin-1
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC010459 GGCCTCGCGCGCCGGGACCCTGCAGAGAAGAACACACCAGTCAGTTTTTCAAGGGAAAATCCTGGGCCAG GAGCCAAGTCCTGATCTCTACAAACTTAATATTGTGTAGATTATTTTACCACCTGAGATCTCAGTGGCCT CATCTGTGCAAGAAAGAGAAAATTAAATAATAAAAAAGATAAAACCTTGGCCTGTCTGAAGATGAGGTGG AGGATCATCCAAGTACAGTACTGTTTTCTCTTGGTTCCGTGCATGCTGACCGCTCTGGAAGCTGTTCCTA TCGATGTGGACAAGACCAAAGTACACAACACTGAGCCAGTGGAAAATGCAAGGATAGAGCCACCAGATAC TGGACTTTATTATGATGAATACCTCAAGCAAGTGATTGAAGTCTTGGAAACAGATCCACATTTCAGAGAA AAGCTCCAGAAAGCAGACATAGAGGAGATAAGGAGCGGGAGGCTGAGTCAAGAGCTGGACTTAGTAAGTC ACAAAGTGAGGACGAGACTGGATGAGCTGAAGAGGCAAGAAGTAGGAAGACTGCGGATGCTCATCAAAGC TAAGCTGGATGCCCTTCAAGACACTGGCATGAATCACCACCTTCTTCTGAAGCAGTTTGAACACCTGAAC CACCAGAATCCTAACACATTTGAATCCAGAGATTTGGATATGCTAATCAAAGCAGCTACCGCGGATCTGG AGCAATATGACCGGACTCGGCATGAAGAGTTTAAGAAGTACGAGATGATGAAGGAACACGAGCGGAGAGA GTATTTAAAAACGCTGAGTGAGGAGAAGAGGAAAGAAGAAGAGTCTAAGTTTGAAGAGATGAAGAGGAAG CACGAAGACCACCCCAAAGTTAATCATCCCGGAAGCAAAGATCAACTAAAAGAGGTTTGGGAAGAGACTG ATGGATTGGACCCTAATGACTTTGACCCCAAGACATTTTTCAAATTACATGATGTTAACAACGATGGATT CCTGGATGAACAAGAATTAGAAGCACTATTCACAAGAGAGTTGGAGAAAGTGTATAACCCACAAAATGCA GAGGACGATATGATAGAAATGGAAGAGGAGAGGCTCAGGATGAGAGAACACGTCATGAGTGAGATTGATA ACAACAAAGACCGATTGGTGACTCTGGAGGAATTCCTGAGAGCTACAGAGAAGAAAGAATTCCTGGAGCC TGATAGCTGGGAGACACTGGACCAGCAACAGTTATTCACCGAGGACGAGCTTAAAGAGTATGAAAGCATT ATTGCTATCCAAGAGAACGAGCTTAAGAAGAGGGCGGAAGAGCTGCAGAAACAGAAGGAGGATCTGCAGC GGCAGCACGACCACCTCGAGGCGCAGAAGCAGGAGTATCATCAGGCCGTCCAGCACCTGGAACAGAAGAA ACTTCAACAAGGCATTGCTCCATCAGGGCCAGCGGGAGAGCTGAAGTTTGAGCCACACACATAAAGTCCT GATGTCTGCCAGAACTTGGAAGAAAACCGTTGATTCAACATCTATTTCATCTTTCAACATCCTTTCTTTT CTCTCCACTCAATAAATACTTTAAAGCACATTTGGAATAAAGGAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	NM_016773
Insert Size	1263 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC010459, AAH10459
RefSeq Size	1598 bp
RefSeq ORF	1263 bp
Locus ID	53322
Gene Summary	Calcium-binding protein. May have a role in calcium homeostasis.[UniProtKB/Swiss-Prot Function]

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