Fzd4 (NM_008055) Mouse Untagged Clone

CAT#: MC203860

Fzd4 (untagged) - Mouse frizzled homolog 4 (Drosophila) (Fzd4), (10ug)



  "NM_008055" in other vectors (4)

CNY 4,008.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
FZD4 Antibody - C-terminal region
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Fz4
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC015256
AGCGCTGGGGCGGTGAGAACAGCGCGGCGTAGAGTGCAGGCGGGCTTCGCCGAAAAGCCGGACTCGGCCG GCGCCGAGTTCTGGGATCGCCGCCTGCAGCCATGACCCTAGCAGTCCATCCCTCGGCCCGGGCTCCGGAC GTCTGATATCCCGCACATTCTCGTACAACTGCTGGAGAGGCGACTGCTGCCCCCTTGTCGCCCTTGGCGC CTTACCGCATTCCCTATCCGGAGTTGGGAGCAGCGCGGCCACCGGCGCCCCTGTGCAAACTGGGGGTGTC TGCTAGATCAGCCTCTGCCGCTGCTGCCCGCAGCTCTGGCCATGGCCTGGCCGGGCACAGGGCCGAGCAG CCGGGGGGCGCCTGGAGGCGTCGGGCTCAGGCTGGGGCTGCTGCTGCAGTTGCTCCTGCTCCTGCGGCCG ACATTGGGGTTCGGGGACGAGGAGGAGCGGCGCTGCGACCCCATCCGCATCGCCATGTGCCAGAACCTCG GCTACAACGTGACCAAGATGCCCAACTTAGTGGGACACGAGCTGCAGACAGACGCCGAGCTGCAGCTGAC AACTTTCACGCCGCTCATCCAGTACGGCTGCTCCAGCCAGCTGCAGTTCTTCCTTTGTTCGGTTTATGTG CCAATGTGCACAGAGAAGATCAACATCCCCATCGGCCCGTGCGGTGGCATGTGCCTTTCAGTCAAGAGAC GCTGTGAACCAGTCCTGAGAGAATTTGGGTTTGCCTGGCCCGACACCCTGAACTGCAGCAAGTTCCCGCC CCAGAACGACCACAACCACATGTGCATGGAAGGACCAGGTGATGAAGAGGTTCCCTTGCCCCACAAGACT CCCATCCAGCCCGGGGAAGAGTGCCACTCCGTGGGAAGCAATTCTGATCAGTACATCTGGGTGAAGAGGA GCCTGAACTGTGTTCTCAAGTGTGGCTACGATGCTGGCTTGTACAGCCGCTCAGCTAAGGAGTTCACGGA TATTTGGATGGCTGTGTGGGCCAGCCTCTGCTTCATCTCCACCACCTTCACCGTGCTGACCTTCCTGATT GATTCATCCAGGTTTTCTTACCCTGAGCGCCCCATCATATTTCTCAGTATGTGCTATAATATTTATAGCA TTGCTTATATTGTTCGGCTGACTGTAGGCCGGGAAAGGATATCCTGTGATTTTGAAGAGGCGGCAGAGCC CGTTCTCATCCAAGAAGGACTTAAGAACACAGGATGTGCAATAATTTTCTTGCTGATGTACTTTTTTGGA ATGGCCAGCTCCATTTGGTGGGTTATTCTGACACTCACTTGGTTTTTGGCAGCCGGACTCAAGTGGGGTC ATGAAGCCATTGAAATGCACAGTTCTTATTTCCACATCGCAGCCTGGGCTATTCCCGCAGTGAAAACCAT TGTCATCTTGATTATGAGACTAGTGGATGCCGATGAACTGACTGGCTTGTGCTATGTTGGGAACCAAAAC CTAGATGCCCTCACTGGCTTTGTGGTGGCTCCTCTCTTTACGTATTTGGTGATTGGAACGCTGTTCATTG CGGCGGGTTTGGTGGCCTTATTCAAAATTCGGTCCAATCTTCAAAAAGACGGGACAAAGACAGACAAGTT GGAAAGGCTAATGGTCAAGATCGGGGTCTTCTCAGTACTGTACACGGTTCCTGCAACCTGTGTGATTGCC TGTTATTTCTATGAAATCTCAAACTGGGCACTCTTTCGATATTCTGCAGATGACTCAAACATGGCAGTTG AAATGTTGAAAATTTTTATGTCTTTGCTCGTGGGCATCACTTCAGGCATGTGGATTTGGTCTGCCAAAAC TCTTCACACGTGGCAAAAGTGTTCTAACCGATTGGTGAATTCTGGGAAGGTAAAGAGAGAGAAGAGGGGG AATGGTTGGGTGAAGCCAGGAAAAGGCAACGAGACTGTGGTATAAGACTAGCCGGCTTCCTCGTTCCTCA TTGTGAAGGAAGTGATGCAGGGAATCTCAGTTTGAACAAACTTAGAAACACTTCAGCCCACACACACCCA CGTCAGCCCACCACCACTCACCCAACTCAGCATCAGAAGACCAATGGCTTCACTGCAGACTTTGGAATGG TCCAAAATGGAAAAGCCAGTTAGAGGTTTTCAAAGCTGTGAAAAATCAAAATGTTGATCACTTTAGCAGG TCACAGCTTGGAGTCCGTGGAGGTCCCGCCTAGATTCCTGAAGCCCAGGGTGATAGTGTTTGCTCCTACT GGGTGGGATTTCAACTGTGAGTTGATAACATGCAAGGAGAAAGATTAATTTTTAAAACCCTTTTAAATTT TAAATAGTAACTAGGTCTTGCAGATAGCAAAGTGATCTATAAACACTGGAAATGCTGGGTTGGGAGACGT GTTGCAGAGTTTTATAGTTTGGCTGGTCTAACATAAACATCTTCTGGCCTACACTGTCTGCTGTTTAGAA CTCTGTAGCGCACTCCCAAGAGGTGGTGTCAAAATCCTTCAGTGCCTTTGTCGTAAAACAGAATTGTTTG AGCAAACAAAAGTACTGTACTAACACACGTAAGGTATCCAGTGGATTTCTCTCTCCTGAAATTTCAACAT CCCTAATTCTAGGCAGCCCCTGTTTTCTTCACTTTAAACTAATGACTCAAAAAAAAAAAGGTTATTTTTA TAGGATTTTTTTTTGCACTGCAGCATGCCTAATGAGAGGAAAAGGGAAGGTGATTCACTTTCTGACAATC ACTTAATTCAGAGAAAAATGAGATTTGCTAAGTTGACTTACCTTACCGACCCTAGAGACCTATTGCATTA AGCAATGTTAAGCAATTGGGACTTAAAATATTTTAGTTTGTGTGATTGCATCTAGGCAGACGCCAGTCTG GAAGAACTGAAATGTTAAATTTCTTGGCAACTTTGCATTCACACAGATTAACTGTGTAATTTGTGTGTGT CAATTACAATTAAAAGCACATTCTTGGACCATGAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_008055
Insert Size 1614 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC015256, AAH15256
RefSeq Size 2989 bp
RefSeq ORF 1614 bp
Locus ID 14366
UniProt ID Q61088
Gene Summary Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activation by Wnt5A stimulates PKC activity via a G-protein-dependent mechanism.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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