Proc (NM_001042767) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	P; PC
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC013896 CCACGCGTCCGGGATTAACCTGACTCCCAGACTGACATGGCGCTACCTGGACGAAATTGCAGTTTCTCCT TGGCCCACGCCTGTGTCAGCAGCTCCAGGATGTGGCAATTCAGAGTCTTCCTGCTGCTCATGTCCACCTG GGGAATATCTAGCATACCGGCCCATCCTGACCCAGTGTTCTCCAGCAGCGAGCATGCCCACCAGGTGCTT CGGGTCAGACGTGCCAACAGCTTCCTGGAAGAGATGCGGCCAGGCAGCCTGGAACGGGAGTGTATGGAGG AGATCTGTGACTTCGAGGAGGCCCAGGAGATTTTCCAAAATGTGGAAGACACACTGGCCTTCTGGATCAA GTACTTTGACGGTGACCAGTGCTCGGCTCCACCCTTGGACCACCAGTGCGACAGCCCATGCTGCGGGCAT GGCACTTGCATCGACGGCATAGGCAGCTTCAGCTGCAGCTGCGATAAGGGCTGGGAGGGCAAGTTCTGTC AGCAGGAGTTGCGCTTCCAGGACTGTCGGGTGAACAATGGCGGCTGCTTGCACTACTGCCTGGAGGAGAG CAATGGGCGGCGCTGCGCTTGTGCCCCGGGCTATGAGCTGGCAGACGACCACATGCGCTGCAAGTCCACT GTGAATTTTCCATGTGGGAAACTGGGGAGGTGGATAGAGAAGAAACGCAAGATCCTCAAACGAGACACAG ACTTAGAAGATGAACTGGAACCAGATCCAAGGATAGTCAACGGAACGCTGACGAAGCAGGGTGACAGTCC TTGGCAGGCAATCCTTCTGGACTCCAAGAAGAAGCTGGCCTGCGGAGGGGTGCTCATCCACACTTCCTGG GTGCTGACGGCAGCCCACTGCGTGGAGGGCACCAAGAAGCTTACCGTGAGGCTTGGTGAGTATGATCTGC GACGCAGGGACCACTGGGAGCTGGACCTGGACATCAAGGAGATCCTCGTCCACCCTAACTACACCCGGAG CAGCAGTGACAACGACATTGCTCTGCTCCGCCTAGCCCAGCCAGCCACTCTCTCCAAAACCATAGTGCCC ATCTGCCTGCCGAACAATGGGCTGGCGCAGGAGCTCACTCAGGCTGGCCAGGAGACAGTGGTGACAGGCT GGGGCTATCAAAGCGACAGAATCAAGGATGGCAGAAGGAACCGCACCTTCATCCTCACCTTCATCCGCAT CCCTTTGGTTGCTCGAAATGAGTGCGTGGAGGTCATGAAGAATGTGGTCTCGGAGAACATGCTGTGTGCA GGCATCATTGGGGACACGAGAGACGCCTGTGATGGTGACAGTGGGGGGCCCATGGTGGTCTTCTTTCGGG GTACCTGGTTCCTGGTGGGCCTGGTGAGCTGGGGTGAGGGCTGTGGGCACACCAACAACTATGGCATCTA CACCAAAGTGGGAAGCTACCTCAAATGGATTCACAGTTACATTGGGGAAAAGGGTGTCTCCCTTAAGAGC CAGAAGCTATAGCACCCCTCCCTGCTCACCTCTGGACCCTAGAAGTCACTCTTGGAGTAAGGCTGGGCTA GTGAGTACCAAGACAGAGGACATTAAAGGAGCATGCAACAAACATAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	NM_001042767
Insert Size	1383 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC013896, AAH13896
RefSeq Size	1603 bp
RefSeq ORF	1383 bp
Locus ID	19123
UniProt ID	P33587
Gene Summary	This gene encodes the vitamin K-dependent protein C, which plays a vital role in the anticoagulation pathway. The encoded protein undergoes proteolytic processing including activation by thrombin-thrombomodulin complex to form the anticoagulant serine protease that degrades activated coagulation factors. A complete lack of the encoded protein in mice results in severe perinatal consumptive coagulopathy in the brain and liver, resulting in death within 24 hours after birth. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate the mature protein. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses an in-frame downstream start codon, compared to variant 1. The encoded isoform (2) is shorter at the N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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