Mapk9 (NM_207692) Mouse Untagged Clone

CAT#: MC204554

Mapk9 (untagged) - Mouse mitogen-activated protein kinase 9 (Mapk9), transcript variant alpha2, (10ug)



  "NM_207692" in other vectors (4)

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI851083; JNK2; p54aSAPK; Prkm9
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC028341
CCACGCGTCCGCCCACGCGTCCGGCAGAAGGGAACCGGACCGGAAGGGTCGCAGCGCCCCGCGCCCCTCA CACCCACTGCGACGGCGACGGGGCGGAGCAGAGCGGGGCGGGGCGACCGGGTCCAGGTCGTGGAGCTGGG TGCCGCCAGAACGCATCGCCGCCAGCGTGCCCTCCCTCTGCCTCCGGCGCGGCGGCGCGGAGCCGAGGGG TCTGACGAAGGCAGCAGCCCTCAGGATCCTGTATGACACTACATCATGAGTGACAGTAAAAGCGATGGTC AGTTTTACAGTGTGCAGGTGGCGGACTCAACTTTCACTGTTCTAAAACGTTACCAGCAACTGAAACCGAT CGGCTCTGGAGCCCAAGGAATTGTTTGTGCTGCTTTTGATACAGTTCTTGGGATAAATGTGGCTGTCAAG AAGTTAAGTCGTCCTTTTCAGAACCAAACGCACGCAAAGAGAGCCTACCGTGAACTCGTCCTCTTAAAGT GTGTCAATCATAAAAACATAATTAGCTTGTTAAATGTGTTCACACCACAAAAAACGCTAGAAGAATTTCA AGATGTGTACTTGGTAATGGAACTAATGGATGCTAACTTATGTCAGGTTATTCATATGGAACTGGACCAT GAGAGAATGTCCTACCTTCTCTACCAGATGCTCTGTGGCATCAAGCATCTGCATTCAGCTGGTATCATTC ATAGAGATTTGAAGCCTAGCAACATTGTAGTAAAATCAGACTGTACCCTCAAGATCCTTGACTTTGGCCT GGCGCGAACAGCCTGTACCAACTTTATGATGACTCCCTATGTGGTAACTCGATACTATCGGGCTCCAGAA GTCATCCTGGGCATGGGCTACAAAGAGAATGTTGATATCTGGTCAGTGGGTTGCATCATGGGAGAGCTGG TGAAAGGTTGTGTGATATTCCAAGGTACTGACCATATTGATCAATGGAATAAAGTTATTGAACAGCTAGG AACACCATCCGCAGAGTTCATGAAGAAACTTCAGCCAACTGTAAGGAATTATGTGGAAAACAGACCAAAG TACCCTGGAATCAAGTTTGAAGAGCTCTTTCCAGATTGGATATTTCCGTCAGAATCTGAGAGAGACAAAA TAAAAACAAGTCAAGCCAGAGATCTGTTATCAAAAATGCTAGTGATTGATCCAGACAAGCGGATCTCTGT GGACGAAGCCTTGCGCCACCCGTATATTACTGTTTGGTATGACCCCGCTGAAGCAGAAGCGCCACCACCT CAAATTTATGATGCCCAGTTGGAAGAAAGAGAGCATGCGATTGAAGAGTGGAAAGAGCTAATTTACAAAG AAGTGATGGACTGGGAAGAAAGAAGCAAGAATGGGGTAAAAGACCAGCCTTCAGATGCAGCAGTAAGTAG CAAGGCTACTCCTTCTCAGTCGTCATCCATCAATGACATCTCATCCATGTCCACTGAGCACACCCTGGCC TCAGACACAGACAGCAGTCTCGATGCCTCAACAGGACCCCTGGAAGGCTGCCGGTGATATGTATGTTGGA GACGGCGCACTTGTCTGTGAAGGAACTCTCACTTCCATGGCCCCGAGCGCGTGGGAGCTGATGGAACCAA ATCGAAAAACTCCATGTTCTGCATGTAAGAAACACGATGCCTTGCCCTGCTCAGTTCCAGTAGGATTGCC TGCTTAGACTATAAAATGAGGCAGACTATGTCTGAAGAAAAAGTACAAACCACACTGTTAGAAATTTTGT TCAAGATCATTTCAGGTGAGCAATTAGAATAGCTGAGTTTCTTTTCCAGTTGTGTGGTATCTGTGGTGAC AGCTCATGTGTAACTGTGGGGACTCGTATGCATGTGACCACAAATGCTTGCTTGAACTTGCCCATGTAGC ACTTTGGGAATCAGCATTTAAATGCCAAATAATCTTGCAGGTAGTTCTGCTTCTAGAATAATCTCTTAAT CCTCTTTAGTAATTTGGTGTCTGTCCACAAAAAAATAGATTATGTGTATTAATTGGCCACCGTCATATAT CATATCTTACCCACTTTATGGTATGATTTATTCTATCTTTTGTATTTCAGAAGGAATATAATTAAATTTA TTTAATAAATAAAAATACAGCTATTCTTAAATTTGTGATGTTTTAGGCTGAGAATTACCACTGCTCTATG TAGACACTCTGTGCCCTTTAAACTGCCCACTGTGGGATACTTTATGTACAGCTTTCTGCATGACAAAGTT CCCAGTTGTATTCCATGCTGCTTAAGACATCTGTCACCTTAAATCCTGCCCACGAATTTCCTTTTTCTTG TTCCTTTGGACATGGATCTAGAATCCTCCACAGAACTTCACCTTCTTTATCACAAAGCACCCCATCTCAA TAGAATGAAGCGGCAGGCTCCTGAACCCACTGCCTAATGTTGGCATAGAGCTGACAGAGTGGCTTCCCCA GAATGGTCATTGAATGGTGCCTCCTTCCCTGAACCCACCCATCCTTTGCTCCCCTCTCCTTATTTAAGGT GAAAGGTGATGGGGTCTCACAGCCGTTCCCTCTGCAGCCTTCCCTCCTGACTTTTCTTTCTCACTGACAG AAGCCACCCCGTCCAGCCAGAGCGCTTGGTCTCCTAGGAGACTGAGCTCACTCACTGTGCCTGTTGGCTG CTGTCTTTTTCCATTTTGTGGAGGCATTTCCTTTTTCTAAGGGAATTCCTCAGATGTTCTAGAAGCACTC AGAAGAACACAGAAGAAATATTCTAGAGAGTAGGGGTTCATTCTTGAATATTTTCTGATTTAAAACTGCT CACCTGAAATTGATACTTTCAGATCCTGATCTGTAAATTACTCAAGATTTGATAAGAGGCTGAGTTCTCT GTGTTGCTGCAGAAATGGAGTTGGGACGGTCATACCGTGGCTACCTGCGCTCAGAGCATTTTCTCCCTTG AGCTTATTTTGTTAACGATTCATATCTTGTGTTCAGTTACTTTGGGAGCTATGGAGTGTGTTCTAGGAGG AAACTTGATAGCTTTTATGAAAAGTACCATCTAATATGTGATTCTGTCTGCACCCGTTGCATCCTAATGG TTGGTCTGATCATTGGAACCTTTAATGGACAACATTAATCTGAAATCTGAAATAAGGATTAGCTTTGTAT CATATATGGATACTTTCAGGTACAGAGTGGAAATGCTAGTGTGGGTGGGGGCTGAGACACCTGTCTATGG CTTCAGGTTATTGGGCCCTGGGAGCCTGAAGTCCCACAGTGCATCAGTCAGCATCATGCTGTGCTCTTCC TGCTGATGCACATTCTTTAAATGACTTCCCGGAAACTTAAGGAGGAAATACAGAAAGGAGCTGTATCATC TACCCTACACTGTAAGGGGAGTGTGCTATGATAGAGGCTCACCCTGGTCCCACGGGCAGGAGACAGCTGT GAGTGGATCCAGGTGCCCCCAAAGAGCGGCTTTCTCTCTCCCCTTACTCTGCATATCCATTGAGTCTGTT GTGGATGGAGTCTGTGCTGGTGTGCTCAGGGTCTTGTGCCAAGACCCTCTTCCATCCAGGCAAGTGCACC TATGACCGGCCTCAGAGCCCCAAAGAAATACCTCACCCCAGTTTCAGGGTCCTCCCTCCTGAGCCTAGAT TTGCCAGGGTCCTGGGACAATGCGAATGGTTTTGAACTAACTTCTGTAGGCAAATGCTGATGTTGGATGA AGAACTGAAAAATGTGCTCAACTTTTTCATCTTTTCTATTTTATTTTTGTAATTTATATTGTACACCCTG GAAGTCACTATTTTGGACATGTATTTTTATAAACCGGGTAACTAATGATTATCTGGAATTTGCACTAGGT TAGTTTTTGTTGTTGTTGGCTTAATTTTCAGCCAAAAGTGGAATGACTGCCCCTTCCAGGTCCGGGTTTC CATTTCAAGCGCAGGTCTGGAGGGCAGACATGTCCAGCCAGGGTAGGAGAGCGGCCTCAGAACACAGGCT GCTCAGTGCCTCGAGCATTGGGCCTGCAGACAAAACCAGGCTGTGCTCTCAACTTTAGAACACAAAGACC AGACCCCCGGTGTGAGAAGGAGAAAGCCCTCCATTGGCCCTTGCTGACAGCCGGGGCCTGCATCTAAAGC AAAAAACCCAACCCTGGTTTCATCTGGGCTGAGTGGTGTGTGAGTCTGTCTGCTGACTCCTCTGATGTCC CATGCCGATAGTCAGTACAGGTGTGACCTTTCAGATCGCGTATCTCAAAAGTGGCTTTGCCTAATGTTTT ATAATAAAATATGTCATGTGCTTTGATTGGTGGAAAATAATACAAACTACATTTTAAAAGAGAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_207692
Insert Size 1272 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC028341, AAH28341
RefSeq Size 4372 bp
RefSeq ORF 1272 bp
Locus ID 26420
UniProt ID Q9WTU6
Gene Summary Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteosomal degradation (PubMed:29153991).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (alpha2) encodes isoform alpha2.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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