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Ppargc1a (NM_008904) Mouse Untagged Clone
CAT＃: MC204780
Ppargc1a (untagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1, (10ug)
CNY 8,768.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | A830037N07Rik; Gm11133; Pgc; PGC-1; Pgc-1alpha; Pgc1; Pgco1; PPARGC-1-alpha; Ppargc1 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC066868
TGAAAAAGCTTGACTGGCGTCATTCGGGAGCTGGATGGCTTGGGACATGTGCAGCCAAGACTCTGTATGG AGTGACATAGAGTGTGCTGCTCTGGTTGGTGAGGACCAGCCTCTTTGCCCAGATCTTCCTGAACTTGACC TTTCTGAACTTGATGTGAATGACTTGGATACAGACAGCTTTCTGGGTGGATTGAAGTGGTGTAGCGACCA ATCGGAAATCATATCCAACCAGTACAACAATGAGCCTGCGAACATATTTGAGAAGATAGATGAAGAGAAT GAGGCAAACTTGCTAGCGGTCCTCACAGAGACACTGGACAGTCTCCCCGTGGATGAAGACGGATTGCCCT CATTTGATGCACTGACAGATGGAGCCGTGACCACTGACAACGAGGCCAGTCCTTCCTCCATGCCTGACGG CACCCCTCCCCCTCAGGAGGCAGAAGAGCCGTCTCTACTTAAGAAGCTCTTACTGGCACCAGCCAACACT CAGCTCAGCTACAATGAATGCAGCGGTCTTAGCACTCAGAACCATGCAGCAAACCACACCCACAGGATCA GAACAAACCCTGCCATTGTTAAGACCGAGAATTCATGGAGCAATAAAGCGAAGAGCATTTGTCAACAGCA AAAGCCACAAAGACGTCCCTGCTCAGAGCTTCTCAAGTATCTGACCACAAACGATGACCCTCCTCACACC AAACCCACAGAAAACAGGAACAGCAGCAGAGACAAATGTGCTTCCAAAAAGAAGTCCCATACACAACCGC AGTCGCAACATGCTCAAGCCAAACCAACAACTTTATCTCTTCCTCTGACCCCAGAGTCACCAAATGACCC CAAGGGTTCCCCATTTGAGAACAAGACTATTGAGCGAACCTTAAGTGTGGAACTCTCTGGAACTGCAGGC CTAACTCCTCCCACAACTCCTCCTCATAAAGCCAACCAAGATAACCCTTTCAAGGCTTCGCCAAAGCTGA AGCCCTCTTGCAAGACCGTGGTGCCACCGCCAACCAAGAGGGCCCGGTACAGCGAGTGTTCTGGTACCCA AGGCAGCCACTCCACCAAGAAAGGGCCCGAGCAATCTGAGTTGTACGCACAACTCAGCAAGTCCTCAGGG CTCAGCCGAGGACACGAGGAAAGGAAGACTAAACGGCCCAGTCTTCGGCTGTTTGGTGACCATGACTATT GTCAGTCACTCAATTCCAAAACGGATATACTCATTAACATATCACAGGAGCTCCAAGACTCTAGACAACT AGACTTCAAAGATGCCTCCTGTGACTGGCAGGGGCACATCTGTTCTTCCACAGATTCAGGCCAGTGCTAC CTGAGAGAGACTTTGGAGGCCAGCAAGCAGGTCTCTCCTTGCAGCACCAGAAAACAGCTCCAAGACCAGG AAATCCGAGCGGAGCTGAACAAGCACTTCGGTCATCCCTGTCAAGCTGTGTTTGACGACAAATCAGACAA GACCAGTGAACTAAGGGATGGCGACTTCAGTAATGAACAATTCTCCAAACTACCTGTGTTTATAAATTCA GGACTAGCCATGGATGGCCTATTTGATGACAGTGAAGATGAAAGTGATAAACTGAGCTACCCTTGGGATG GCACGCAGCCCTATTCATTGTTCGATGTGTCGCCTTCTTGCTCTTCCTTTAACTCTCCGTGTCGAGACTC AGTGTCACCACCGAAATCCTTATTTTCTCAAAGACCCCAAAGGATGCGCTCTCGTTCAAGATCCTTTTCT CGACACAGGTCGTGTTCCCGATCACCATATTCCAGGTCAAGATTAAGGTCCCCAGGCAGTAGATCCTCTT CAAGATCCTGTTACTACTATGAATCAAGCCACTACAGACACCGCACACACCGCAATTCTCCCTTGTATGT GAGATCACGTTCAAGGTCACCCTACAGCTGTAGGCCCAGGTACGACAGCTATGAAGCCTATGAGCACGAA AGGCTCAAGAGGGATGAATACCGCAAAGAGCACGAGAAGCGGGAGTCTGAAAGGGCCAAGCAGAGAGAGA GGCAGAAGCAGAAAGCAATTGAAGAGCGCCGTGTGATTTACGTTGGTAAAATCAGACCTGACACAACGCG GACAGAATTGAGAGACCGCTTTGAAGTTTTTGGTGAAATTGAGGAATGCACCGTAAATCTGCGGGATGAT GGAGACAGCTATGGTTTCATCACCTACCGTTACACCTGTGACGCTTTCGCTGCTCTTGAGAATGGATATA CTTTACGCAGGTCGAACGAAACTGACTTCGAGCTGTACTTTTGTGGACGGAAGCAATTTTTCAAGTCTAA CTATGCAGACCTAGATACAAACTCAGACGATTTTGACCCTGCTTCCACCAAGAGCAAGTATGACTCTCTG GATTTTGATAGTTTACTGAAGGAAGCTCAGAGAAGCTTGCGCAGGTAACATGTTCCCAGGCTGAGGAATG ACAGAGAGATGGTCAATACCTCATGGGACAGCGTGTCCTTTCCCAAGACTCTTGCAAGTCATACTTAGGA ATTTCTCCTACTTTACACTCTCTGTACAAAAATAAAACAAAACAAAACAACAATAACAACAACAACAACA ACAACAACAATAACAACAACAACCATACCAGAACAAGAACAACGGTTTACATGAACACAGCTGCTGAAGA GGCAAGAGACAGAATGATAATCCAGTAAGCACACGTTTATTCACGGGTGTCAGCTTTGCTTTCCCTGGAG GCTCTTGGTGACAGTGTGTGTGCGTGTGTGTGTGTGGGTGTGCGTGTGTGTATGTGTGTGTGTGTACTTG TTTAGAAAGTACATATGTACACATGTGAGGACTTGGGGGCATCTGAACAGAACGAACAAGGGCGACCCCT TCAAATGGCAGCATTTCCATGAAGACACACTTAAAACCTACAACTTCAAAATGTTCGTATTCTATACAAA AGGAAAATAAATAAATATAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_008904 |
| Insert Size | 2394 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC066868, AAH66868 |
| RefSeq Size | 2978 bp |
| RefSeq ORF | 2394 bp |
| Locus ID | 19017 |
| UniProt ID | O70343 |
| Gene Summary | This gene encodes a transcriptional coactivator that induces and coordinates gene expression regulating mitochondrial biogenesis, respiration, hepatic gluconeogenesis, thermogenic program in brown fat and muscle fiber-type switching. Mice lacking the encoded protein exhibit reduced thermogenic capacity, hyperactivity and resistance to diet-induced obesity. Mice lacking the encoded protein specifically in the heart exhibit peripartum cardiomyopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (1) encodes a functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210710 | Ppargc1a (tGFP-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a) |
CNY 10,352.00 |
|
| MR210710 | Ppargc1a (Myc-DDK-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1 |
CNY 8,752.00 |
|
| MR210710L1 | Lenti ORF clone of Ppargc1a (Myc-DDK-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1 |
CNY 9,880.00 |
|
| MR210710L2 | Lenti ORF clone of Ppargc1a (mGFP-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1 |
CNY 11,152.00 |
|
| MR210710L3 | Lenti ORF clone of Ppargc1a (Myc-DDK-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1 |
CNY 11,152.00 |
|
| MR210710L4 | Lenti ORF clone of Ppargc1a (mGFP-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1 |
CNY 11,152.00 |
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