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Bckdhb (NM_199195) Mouse Untagged Clone
CAT＃: MC204787
Bckdhb (untagged) - Mouse branched chain ketoacid dehydrogenase E1, beta polypeptide (Bckdhb), nuclear gene encoding mitochondrial protein, (10ug)
CNY 2,400.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | BCKDE1B; BCKDH E1-beta |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC064099
CAGAATTGGATGGCTGAATTTGAACAGATCCTTCGGGAATTGAGACTTCAGGTCAACTCCACGCGCTTGG ACCTGTCGCTGACCAAAGGATTACCCAATTGGATCTCCTCAGCATTTTCTTTCTTTAAAAAATAGGGCAA ACTCAGAAGATGAACCTCTTCCAGTCAATAACAAGTGCCCTGGATAACTCATTAGCCAAAGACCCCACTG CAGTAATATTTGGTGAAGATGTTGCCTTTGGTGGAGTCTTCCGATGCACTGTTGGTTTACGAGACAAATA CGGAAAAGATAGAGTGTTTAACACCCCGTTGTGTGAACAAGGAATAGTTGGATTTGGCATTGGAATCGCG GTCACCGGTGCTACAGCTATTGCGGAAATCCAGTTTGCCGACTATATTTTCCCTGCCTTTGATCAGATTG TCAACGAAGCTGCCAAGTATCGCTACCGCTCAGGTGATCTTTTCAACTGTGGGAGCCTCACCATCCGGGC CCCGTGGGGTTGTGTGGGCCATGGGGCTCTCTACCATTCTCAGAGTCCTGAAGCCTTTTTTGCCCATTGC CCAGGGATCAAGGTGGTAATACCCCGAAGCCCTTTCCAGGCCAAGGGACTTCTGTTGTCATGCATAGAAG ATAAAAATCCATGTATATTTTTTGAACCTAAAATACTTTACCGGGCAGCAGTGGAACAGGTCCCAGTAGA ACCCTACAAGATCCCCTTGTCTCAGGCTGAAGTCATCCAGGAGGGCAGCGATGTGACTCTGGTTGCCTGG GGCACTCAGGTTCATGTCATCCGGGAGGTGGCTTCCATGGCCCAAGAAAAGCTTGGAGTATCTTGTGAAG TCATCGATCTGCGGACAATTGTGCCTTGGGATGTGGATACAGTTTGCAAGTCTGTGATCAAAACCGGGCG ACTGTTGATCAGCCACGAGGCTCCCTTAACAGGCGGCTTTGCCTCTGAGATCAGCTCCACGGTCCAGGAA GAATGTTTCTTGAACCTAGAGGCTCCAATATCTCGAGTTTGCGGATATGACACCCCGTTTCCTCACATCT TTGAGCCCTTTTATATCCCAGACAAATGGAAGTGCTACGATGCCCTTCGCAAGATGATCAACTATTGACG ACAGAGAAAACCAGGAAGATCATGACCAGACATGGAAATATTTTTCTGAAACCTTTTTATATTTCCTTAT ACTTCTTTCTTCTTACATATAGTTTTATGCAACGAACTGTCATGGATATTGGCTGATGAGCTGCGAATTA CTTATCATGTTAATATAAATGGATTGAGTGCACAGGAGGAATGCTTTAAGGAGTGCGTGGGCGGTGATCT TAAGACTGTTTCATGACTATAAATACCGGGTGGTGTTGTATTCAATAAAGCCAAATTACATCGCCTCAAA AAAAAAAAAAAAAAAAAAA |
| Restriction Sites | EcoRI-NotI |
| ACCN | NM_199195 |
| Insert Size | 969 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC064099, AAH64099 |
| RefSeq Size | 1419 bp |
| RefSeq ORF | 969 bp |
| Locus ID | 12040 |
| UniProt ID | Q6P3A8 |
| Gene Summary | This gene encodes the beta chain of the branched chain alpha ketoacid dehydrogenase (Bckdh) complex. The encoded protein exists in a heterotetrameric complex containing the Bckdh alpha subunit to form the E1 catalytic component of Bckdh complex. The Bckdh complex catalyzes the oxidative decarboxylation of branched chain ketoacids to their corresponding acyl-CoA esters, during the catabolism of leucine, isoleucine and valine. In humans, certain mutations in this gene cause maple syrup urine disease. Alternative splicing results in multiple transcript variants encoding different isoforms. A pseudogene for this gene has been identified. [provided by RefSeq, Apr 2015] Transcript Variant: This variant (2) contains an alternate 5' terminal exon and uses a downstream start codon compared to variant 1. It encodes isoform 2 which has a shorter N-terminus compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR204658 | Bckdhb (Myc-DDK-tagged) - Mouse branched chain ketoacid dehydrogenase E1, beta polypeptide (Bckdhb), nuclear gene encoding mitochondrial protein |
CNY 3,230.00 |
|
| MR204658L3 | Lenti ORF clone of Bckdhb (Myc-DDK-tagged) - Mouse branched chain ketoacid dehydrogenase E1, beta polypeptide (Bckdhb), nuclear gene encoding mitochondrial protein |
CNY 5,130.00 |
|
| MR204658L4 | Lenti ORF clone of Bckdhb (mGFP-tagged) - Mouse branched chain ketoacid dehydrogenase E1, beta polypeptide (Bckdhb), nuclear gene encoding mitochondrial protein |
CNY 5,130.00 |
