Atraid (NM_212470) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	0610007C21Rik; AI316792; Apr3; HSPC013; p18
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC049637 GAAAGCAGTGGGAAATGGCGTCTCGGGAGTCAGGCGGCTCTCGGGCGGCCGCCCTGCTCCTGGTTCTGGG CGTGGAGAGGGCCCTGGCTCTACCTGAGATATGCACCCTGTGTCCGGGAGGCTAGATCTTCAGAACTGTT CCCTGAAGGATCCTGGTCCAAACTTTTTACAGGCTTATACTGCTATTATCATAGACCTTCAGGCAAATCC TCTCAAGGATGATTTGGCCAACACCTTCCGTGGGTTTACTCAGCTGCAGACTCTGATACTACCACAAGAT GTTCCCTGTCCTGGAGGTAGTAATGCCTGGGACAATGTTACTTCTTTCAAGGACAAGCAGATTTGCCAAG GGCAAAGGGACCTTTGCAATAGCACTGGAAGCCCAGAAATGTGTCCTGAGAACGGATCTTGTGCATCTGA CGGTCCTGGTCTTTTGCAGTGCGTTTGTGCTGATGGTTTCCATGGATACAAGTGTATGAGGCAGGGCTCA TTTTCACTGCTTATGTTCTTTGGGATTCTGGGATCCACCACGCTAGCCATCTCCATTTTACTTTGGGGAA CCCAGCGCCGGAAAGCCAAGGCTTCATGAGCCACACAAGACTTCCAGCTCACCTGACTGCGCACCCTCAT CTATCAAGTCCCGGCCATGTCGCAGCCAAGAAGATTTACTTCGTTGACAATTACTGGCCAATGAGCAGAA CTGTTTAAGGCTGAAGCGGCCATTGATAATGAAAGGTGTCAAATTGCACCACCTCGGGGGTCTTGTGGAC ACTTGGTTCAGGAGTGGACTCGTACTAATTTGTATTATTGACTACAATAAACACCTTTTCCCCCGTTTTA AAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	SfiI-SfiI
ACCN	NM_212470
Insert Size	489 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC049637, AAH49637
RefSeq Size	896 bp
RefSeq ORF	489 bp
Locus ID	381629
UniProt ID	Q6PGD0
Gene Summary	Promotes osteoblast cell differentiation and terminal mineralization. Plays a role in inducing the cell cycle arrest via inhibiting CCND1 expression in all-trans-retinoic acid (ATRA) signal pathway (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct N-terminus, compared to isoform 1.

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