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Ano1 (NM_178642) Mouse Untagged Clone
CAT＃: MC205263
Ano1 (untagged) - Mouse anoctamin 1, calcium activated chloride channel (Ano1), (10ug)
CNY 7,008.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Tmem16a |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC205263
CAGCGACATCGGCCTGGAGGGCTTGACCCCAGAGGGAACCTCTGCGGACCGAGAGTGCCAGCGGGGACCG GAAACAATCGCGCACGAGGCACAGGACGCAGGAACCCCCAACTCAGGTGACGCCACCGGGGTTGTGGATG GGGAGCGCGAGGCCACCATGAGGGTCCCCGAGAAGTACTCGACGCTCCCGGCGGAGGACCGCAGCGTCCA CATCGTGAACATCTGCGCCATCGAGGACCTGGGCTACCTGCCGTCCGAGGGCACGTTGCTGAACTCTCTG TCCGTGGACCCCGACGCCGAATGCAAGTATGGACTATACTTCAGGGATGGCAAACGGAAGGTGGACTACA TCTTGGTATACCATCACAAGAGAGCCTCAGGGAGCAGGACTCTGGCCAGGAGGGGACTACAAAATGACAT GGTCCTGGGGACCCGCAGCGTCAGGCAGGACCAGCCCCTTCCCGGGAAGGGGAGCCCTGTGGATGCAGGC TCACCGGAAGTCCCCATGGATTACCATGAAGATGACAAACGCTTCAGACGGGAGGAATATGAGGGCAACC TGTTGGAGGCAGGCCTGGAGTTGGAGAATGACGAGGATACCAAAATCCATGGTGTCGGGTTTGTGAAGAT CCATGCGCCCTGGCATGTGCTCTGTAGGGAAGCTGAGTTTTTGAAACTAAAGATGCCCACAAAGAAGGTG TACCACATCAGTGAGACGCGAGGCCTCCTGAAAACCATCAACTCGGTTCTGCAGAAGATCACAGACCCCA TCCAGCCCAAGGTGGCTGAGCACAGGCCACAGACCACAAAGAGGCTCTCCTATCCCTTCTCCCGGGAGAA GCAACACCTATTCGACCTGACTGACAGGGACTCTTTTTTCGACAGCAAAACCCGGAGCACAATAGTCTAT GAAATCCTGAAGAGAACAACGTGCACCAAGGCCAAGTACAGCATGGGTATCACCAGCCTCCTGGCCAATG GCGTATACTCAGCTGCATACCCTCTGCACGATGGGGACTATGAGGGTGACAACGTTGAGTTCAACGACAG GAAACTCCTGTATGAGGAATGGGCAAGTTACGGAGTCTTCTACAAATACCAGCCCATTGACCTGGTCAGG AAATACTTTGGTGAGAAGGTTGGCCTGTACTTTGCCTGGCTTGGAGCCTACACCCAGATGCTCATCCCTG CCTCGATCGTGGGTGTCATTGTCTTTCTCTATGGATGTGCCACTGTGGACGAAAACATCCCCAGTATGGA GATGTGTGACCAGAGATACAACATCACCATGTGTCCTCTGTGTGACAAGACCTGCAGCTACTGGAAGATG AGCTCAGCCTGTGCCACAGCCCGTGCCAGTCACCTTTTTGATAACCCTGCCACCGTCTTCTTCTCTGTGT TTATGGCCCTCTGGGCTGCCACTTTCATGGAGCACTGGAAACGGAAGCAGATGAGGCTCAACTACCGATG GGACCTCACAGGCTTCGAGGAGGAGGAGGATCATCCCAGAGCAGAGTATGAAGCCAGAGTCTTAGAGAAG TCACTGAGAAAAGAATCCAGAAACAAAGAGACCGACAAGGTGAAGCTGACCTGGAGGGACCGATTCCCAG CCTATTTCACCAATCTTGTCTCCATCATCTTCATGATCGCAGTGACATTTGCAATCGTCCTCGGAGTTAT CATCTATAGAATCTCCACAGCTGCAGCCTTGGCCATGAACTCCTCCCCGTCTGTGCGGTCCAACATCCGG GTTACAGTCACGGCCACCGCTGTTATCATCAACCTCGTGGTCATCATTCTGCTGGATGAAGTTTACGGCT GCATTGCCAGGTGGCTCACCAAGATTGAGGTCCCAAAGACAGAGAAGAGCTTTGAGGAGAGGCTAACCTT CAAGGCCTTCCTGCTCAAGTTTGTGAACTCTTACACTCCCATCTTCTATGTCGCCTTCTTCAAAGGCCGG TTTGTTGGTCGGCCCGGTGACTACGTGTACATCTTCCGCTCTTTCCGGATGGAGGAGTGTGCCCCGGGCG GCTGCCTCATGGAGCTCTGTATCCAGCTGAGCATCATTATGCTGGGCAAGCAGCTAATCCAGAACAATCT CTTCGAGATTGGCATCCCGAAGATGAAAAAGTTCATCCGCTACCTGAAGCTGCGCAGACAGAGCCCCTCA GACCGTGAAGAGTACGTGAAGCGGAAGCAGCGCTATGAGGTGGACTTCAACCTCGAACCTTTCGCCGGCC TCACGCCCGAGTACATGGAAATGATCATTCAGTTCGGCTTTGTCACCCTGTTTGTTGCGTCCTTCCCTCT GGCTCCACTCTTCGCCCTGCTAAACAACATCATTGAGATCCGCCTGGATGCCAAAAAGTTTGTCACCGAG CTACGGAGGCCAGTAGCCATCAGAGCCAAAGACATCGGCATCTGGTATAACATCCTCAGAGGTGTTGGGA AGCTGGCTGTCATCATTAATGCCTTTGTGATCTCCTTCACGTCTGACTTCATCCCTCGCCTGGTGTACCT CTACATGTACAGTCAGAATGGGACCATGCACGGCTTCGTCAATCACACGCTCTCTTCCTTCAATGTCAGC GACTTCCAGAATGGCACAGCACCCAATGACCCACTGGACCTGGGCTATGAGGTTCAGATCTGCAGGTATA AAGATTACCGGGAACCCCCATGGTCAGAACACAAGTATGACATCTCCAAAGACTTCTGGGCTGTCCTGGC CGCCCGACTGGCATTTGTCATTGTCTTCCAGAACCTGGTGATGTTCATGAGTGACTTTGTGGACTGGGTG ATCCCTGATATCCCCAAAGACATCAGCCAGCAGATCCACAAAGAGAAGGTTCTCATGGTGGAGCTGTTTA TGCGTGAGGAGCAGGGCAAGCAGCAGCTACTGGACACATGGATGGAGAAGGAGAAGCCAAGGGATGTGCC TTGTAACAACCACAGCCCCACAACCCACCCAGAGGCAGGCGACGGCAGCCCAGTCCCCAGCTACGAGTAC CATGGGGACGCGCTGTAGCCATGATGACAGGCCAGCAGGCCAGCAGGCTTCTGACCATCACCAAGTGACT ACTCGGCTTCCTCCAAAGCCAGCACTTCTAGCCCCACCAGGGCCTATGGCTCTTGTGTCGTCTGGACGTG TGGAGGAGCACTCTGATGAGACAACGGTCTTCTTTCTTCTTTCTGTTTCTTCTCATTTTTGCACAAGGCC ATTATGCAGGGAAGTTTTTATCTTTAGTATTCAATGCCAAGGATGGCTGGTGATGGGGATGAACAGGGTG AGGCATGTTCCACAGACATATGGAGGAATATAGAGCCCCCCTGTGGTGGTAGGGAGCCAAGTCAGCATCC TTAGTCTGAAGGATGCCCTGGGATAGATGCAGAATGGTACCTAGGAACCTGCTGACATTTTGCAGAAGGG TGAACAACTCAGACTCAGATTCCCAAAGATGTCACCTGCTCTAGCTGGACAAGCTGGAAGACTCCACTGT CTTTCCTTCTGGGAGCTCAGAAGTTCAGACCTGGGGTCAGAAACAGAGGTGCCTGACCACCCACACAGCA CACACATATGGTGGCTTCACATTGTACTCCAGTAACGGAGGAGTACAGGGGCTCCCAGTTGTCCAGTACC CTTGGGGTCCCTGGCAGGCTGCACACAAGCCACTCAAGGGCCCCTCACATGAAGCTGATGTCCTCCTTGT GAGACAAGCTGTCGTGACAAGCAGGGCATCTCTAAACAGGCTGGAGGGCTTCACCAAGCCCTGGGACCTC TGTGGGTTCATTTTTTTTTGCTTTTTAAACCATCTATTGGTTTTTGTCTAAAAATCAATAATGTGATGGA TTTGAGGGATTTTCTTTCTGTAGCTGAAGGTGGAAGGAGTTTATTTCATTAGTTAACCAAATACTGAGAG GAAATTAAAATATCATTACTACCAAAGATTTTTCTCAATAAAAGCTTCTATTTTGGTAACACACCTATAT TTTCACTCGTGGGGATGAGACCCTCAGCACTTACCATTGTAATAGCTTCTAAAACAAGCCTCATGGGCCA GCTTCATTTGATGTGCTAGCCAGCTGTACAAGGCCTCTCGGCACCATTGCCAGAAGTGTCTCTCTCGCCC AAGCCGAAGAAGGCAATTCCCTGCACTCGTCTTAGGGCAAGGCAGACAGTGCTGTAGTTGTGAGCTGGGG TCCGAATCTCAGGCTTCCAAGGTTTGCACTGTTTGCTAAAATCGTGAGACCTGATGCTTCTATTTTCTCT TGTGCAGTGATGTGGTTTCCAATGATGTTTTGTATATAGCTACTGTATCCAAAATTAGATAATTACCAAA AATTCTTTTACAGAAACCATTTTTAAAAAACAACAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | NM_178642 |
| Insert Size | 2871 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_178642.4, NP_848757.3 |
| RefSeq Size | 4393 bp |
| RefSeq ORF | 2871 bp |
| Locus ID | 101772 |
| UniProt ID | Q8BHY3 |
| Gene Summary | Calcium-activated chloride channel (CaCC) which plays an important role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211299 | Ano1 (tGFP-tagged) - Mouse transmembrane protein 16A (Tmem16a) |
CNY 9,392.00 |
|
| MR211299 | Ano1 (Myc-DDK-tagged) - Mouse anoctamin 1, calcium activated chloride channel (Ano1) |
CNY 10,170.00 |
|
| MR211299L3 | Lenti ORF clone of Ano1 (Myc-DDK-tagged) - Mouse anoctamin 1, calcium activated chloride channel (Ano1) |
CNY 12,070.00 |
|
| MR211299L4 | Lenti ORF clone of Ano1 (mGFP-tagged) - Mouse anoctamin 1, calcium activated chloride channel (Ano1) |
CNY 12,070.00 |
