Scap (NM_001001144) Mouse Untagged Clone

CAT#: MC205292

Scap (untagged) - Mouse SREBF chaperone (Scap), transcript variant 1, (10ug)



  "NM_001001144" in other vectors (4)

CNY 8,888.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 9530044G19; mKIAA0199
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC070437
GTTGAGAGGTGAAGGGGCGGGGAGCTGCGCGGGCGCCGGGCGGCCGGGAGGGAGAGGGCGGGCTCCAAAC ACCGGACCGCGGGCCAGGAGCGCGCAGGCCGTTCTCCGCCGCTCGGTCGCCGCCGCCCGGGAGCTGCCTC GCTGCCACAGGTGCCTGCAGATGATGTCTGCTGTAAGTGATATCCAGCATCTTCCGGGCTGATCCATGGT CACTTTCCGGGATGGCAACAAGGTGACTTAGCCGAGGATGACCCTGACTGAAAGGCTTCGTGAGAAGATA TCTCAGGCCTTCTACAACCATGGGCTGCTCTGCGCATCCTATCCAATTCCCATCATCCTCTTCACAGGAC TCTGCATCTTAGCCTGCTGCTACCCGCTGCTGAAGCTCCCCTTGCCTGGAACGGGACCTGTGGAATTCTC CACGCCTGTGAAGGGTTACTCGCCCCCGCCTGCGGACTCTGACCACAAACAAGGAGAGCCCAGCGAGCAG CCAGAGTGGTATGTGGGTGCCCCCGTGGCGTACATCCAACAGATATTTGTGAAGTCATCGGTGTCTCCCT GGCACAGAAATCTTCTGGCAGTCGATGTGTTCCGGTCACCTCTGTCCCGAGCATTCCAACTGGTGGAAGA GATCCGGAACCATGTGCTGAGAGACAGCTCAGGGACCAAGAGCCTGGAGGAGGTTTGCCTGCAGGTGACA GACCTGCTGCCAGGCCTCAGGAAACTCCGGAGCCTACTTCCCGAACATGGCTGCCTGCTGCTGTCCCCTG GGAACTTCTGGCAGAATGATTGGGAGAGATTCCATGCCGACCCTGACATCATTGGGACCATCCATCAACA TGAGCCCAAAACTCTACAGACATCAGCCACACTCAAAGACTTGCTGTTTGGTGTTCCTGGGAAGTACAGT GGGGTGAGCCTCTACACAAGGAAAAGGATGGTCTCCTACACCATCACCCTGGTCTTCCAGCGCTACCATG CCAAGTTTCTGAGCAGCCTACGTGCCCGGCTCATGCTGCTGCACCCCAGCCCCAACTGCAGCCTCCGAGC AGAGAACCTGGTCCACGTCCACTTCAAAGAGGAGATTGGCATTGCTGAGCTCATCCCGCTCGTGACCACC TACATCATCCTGTTTGCCTACATCTACTTCTCCACACGCAAGATCGACATGGTCAAGTCCAAGTGGGGCC TCGCCCTGGCAGCCGTGGTCACAGTACTTAGCTCACTGCTCATGTCTGTGGGGCTCTGCACCCTCTTCGG CCTGACGCCCACACTCAATGGCGGTGAGATCTTCCCATACCTGGTGGTCGTTATTGGGCTAGAGAACGTG TTGGTGCTCACCAAGTCAGTGGTATCAACTCCAGTGGACCTCGAGGTGAAGCTTCGGATTGCACAAGGCT TGAGTAGTGAGAGCTGGTCCATCATGAAGAACGCGGCGACCGAGCTGGGCATCATCCTCATTGGCTACTT CACCCTCGTGCCTGCTATCCAGGAGTTCTGCCTCTTTGCTGTTGTGGGCCTGGTGTCTGACTTCTTCCTC CAGATGCTGTTCTTCACCACTGTCCTGTCGATCGACATTCGCCGGATGGAGCTAGCAGACCTAAACAAGC GGCTGCCCCCTGAATCCTGCCTGCCCTCAGCCAAGCCCGTGGGGAGGCCAGCACGATATGAGAGACAGCA GGCTGTACGGCCATCCACGCCACACACCATCACATTGCAACCATCTTCCTTCCGAAACCTGCGGCTTCCC AAAAGGCTGCGTGTCATCTACTTCCTGGCCCGCACTCGCCTGGCCCAGCGCCTCATCATGGCTGGTACCG TTGTCTGGATTGGCATCCTGGTATACACCGACCCGGCAGGGCTGCGCACCTACCTTGCTGCCCAGGTGAC AGAGCAGAGCCCACTGGGTGAGGGTTCCCTGGGCCCCATGCCTGTGCCTAGCGGAGTGCTGCCTGCCAGC CACCCGGACCCTGCATTCTCCATCTTCCCACCTGATGCTCCTAAACTGCCAGAGAACCAGACCTTGCCAG GTGAGCTGCCTGAGCATGCTGGTCCAGCAGAGGGTGTCCATGACAGCCGAGCCCCAGAGGTAACTTGGGG GCTTGAGGATGAGGAGCTGTGGAGGAAATTGTCCTTCCGCCACTGGCCCACACTCTTCAACTACTACAAC ATCACACTGGCCAAAAGGTACATCAGCCTGCTGCCTGTCATCCCTGTCACACTACACCTGAATCCACGGG AGGCTCTGGAGGGGCGACACCCTCAGGATGGTCGCAGTGCCTGGGCCCCACAAGAGCCTTTGCCCGCTGG CCTCTGGGAGTCCGGACCTAAGGGACCAGGTGGAACACAGACCCATGGCGACATTACCTTGTACAAGGTG GCCGCGCTTGGCCTAGCAGCGGGCATCGTCCTGGTGCTGCTGCTGCTCTGCCTCTACCGGGTGCTCTGCC CGCGTAATTATGGGCAGCCGGGTGGTGGCCCCGGCAGGCGGAGGCGCGGGGAGCTGCCCTGCGATGACTA CGGCTACGCACCGCCCGAGACGGAGATAGTGCCGCTGGTGCTGCGAGGTCACCTCATGGACATCGAGTGT CTGGCTAGCGATGGGATGCTACTAGTGAGCTGCTGCCTGGCAGGCCAAGTCTGCGTGTGGGACGCTCAGA CAGGGGACTGCCTCACACGGATCCCACGCCCAGGGCCACGCCGGGATAGCTGCGGAGGTGGAGCTTTTGA GACTCAGGAGAACTGGGAAAGGCTGTCAGATGGAGGCAAGGCTAGCCCGGAAGAACCTGGAGACAGCCCT CCGCTGCGACGACGCCCCCGAGGGCCTCCACCGCCTTCCCTCTTTGGGGACCAGCCCGACCTCACCTGCT TAATCGACACCAACTTCTCGGTGCAGCTGCCCCCAGAGCCCACTCAGCCCGAGCCTCGGCACCGGGTGGG CTGTGGCCGCTCTAGAGACTCGGGTTATGACTTCAGCCGCCTGGTGCAGCGTGTGTACCAGGAGGAAGGC CTGGCTGCTATGCGCATGCCGGCCCTGCGCCCACCCTCCCCTGGACCTCCCTTGCCCCAGGCCTCTCAAG AAGAGGGGACTGCACCTGAGAAGGGCTCCCCTCCCCTGGCCTGGACCCCCAGCACAGCCGGTTCCATCTG GAGCTTAGAGCTGCAAGGCAATCTCATCGTGGTTGGGCGGAGCAGCGGCCGGCTGGAGGTGTGGGACGCC ATTGAGGGGGTGCTCTGCTGCAGCAATGAGGAGATCTCCTCAGGCATCACAGCCCTTGTCTTCTTGGACA GGAGGATTGTAGCTGCTCGGCTTAATGGTTCCCTTGATTTCTTTTCTTTGGAGACCCACACTTCCCTCAG CCCCCTGCAGTTCAGAGGGACCCCAGGGCGAGGCAGTTCTCCTTCCTCATCTGTGTACAGCAGCAGCAAC ACAGTGACCTGTCATCGGACCCACACAGTGCCCTGTGCACACCAGAAGCCCATCACAGCCCTGAGAGCTG CTGCCGGGCGCCTAGTGACAGGGAGCCAAGACCATACTCTAAGAGTCTTCCGACTGGATGACTCGTGTTG CCTCTTTACCCTGAAGGGCCACTCAGGGGCAATCACAGCTGTGTACATTGATCAGACCATGGTACTGGCC AGTGGAGGACAAGATGGAGCCATCTGCCTGTGGGATGTACTAACAGGCAGCCGGGTCAGCCAAACATTTG CTCACCGTGGAGATGTTACCTCCCTCACCTGTACCGCTTCCTGTGTCATTAGTAGTGGCCTGGATGACTT CATCAGTATCTGGGACCGCAGCACAGGCATCAAGCTGTACTCCATTCAGCAGGACCTGGGCTGTGGTGCA AGCTTGGGTGTCATCTCAGATAACCTTCTGGTGACCGGCGGCCAGGGCTGTGTCTCCTTTTGGGACCTAA ACTATGGGGACCTGTTACAGACAGTCTACTTGGGCAAGAACAGTGAAGCCCAGCCTGCCCGGCAGATTTT GGTGTTGGACAATGCTGCCATTGTCTGCAACTTTGGCAGTGAGCTCAGCCTAGTGTATGTGCCCTCTGTG CTGGAGAAACTGGACTGAAGGCAGGTCAAGTACGCTATTCCCTTTCCCCCATCCCAAGGTGGGGCACAGG GGATAGCAACTCTTTGGACCTAGACTAGAGGCAATAGCTGACTCTGAACTGTTGTCTCCTGACTGTAATA ATAAACTTTTTTAAAAAACCACAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI     
ACCN NM_001001144
Insert Size 3831 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC070437, AAH70437
RefSeq Size 4247 bp
RefSeq ORF 3831 bp
Locus ID 235623
UniProt ID Q6GQT6
Gene Summary Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...