United States
Germany
Japan
United Kingdom
China
Scap (NM_001001144) Mouse Untagged Clone
CAT＃: MC205292
Scap (untagged) - Mouse SREBF chaperone (Scap), transcript variant 1, (10ug)
CNY 8,888.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 9530044G19; mKIAA0199 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC070437
GTTGAGAGGTGAAGGGGCGGGGAGCTGCGCGGGCGCCGGGCGGCCGGGAGGGAGAGGGCGGGCTCCAAAC ACCGGACCGCGGGCCAGGAGCGCGCAGGCCGTTCTCCGCCGCTCGGTCGCCGCCGCCCGGGAGCTGCCTC GCTGCCACAGGTGCCTGCAGATGATGTCTGCTGTAAGTGATATCCAGCATCTTCCGGGCTGATCCATGGT CACTTTCCGGGATGGCAACAAGGTGACTTAGCCGAGGATGACCCTGACTGAAAGGCTTCGTGAGAAGATA TCTCAGGCCTTCTACAACCATGGGCTGCTCTGCGCATCCTATCCAATTCCCATCATCCTCTTCACAGGAC TCTGCATCTTAGCCTGCTGCTACCCGCTGCTGAAGCTCCCCTTGCCTGGAACGGGACCTGTGGAATTCTC CACGCCTGTGAAGGGTTACTCGCCCCCGCCTGCGGACTCTGACCACAAACAAGGAGAGCCCAGCGAGCAG CCAGAGTGGTATGTGGGTGCCCCCGTGGCGTACATCCAACAGATATTTGTGAAGTCATCGGTGTCTCCCT GGCACAGAAATCTTCTGGCAGTCGATGTGTTCCGGTCACCTCTGTCCCGAGCATTCCAACTGGTGGAAGA GATCCGGAACCATGTGCTGAGAGACAGCTCAGGGACCAAGAGCCTGGAGGAGGTTTGCCTGCAGGTGACA GACCTGCTGCCAGGCCTCAGGAAACTCCGGAGCCTACTTCCCGAACATGGCTGCCTGCTGCTGTCCCCTG GGAACTTCTGGCAGAATGATTGGGAGAGATTCCATGCCGACCCTGACATCATTGGGACCATCCATCAACA TGAGCCCAAAACTCTACAGACATCAGCCACACTCAAAGACTTGCTGTTTGGTGTTCCTGGGAAGTACAGT GGGGTGAGCCTCTACACAAGGAAAAGGATGGTCTCCTACACCATCACCCTGGTCTTCCAGCGCTACCATG CCAAGTTTCTGAGCAGCCTACGTGCCCGGCTCATGCTGCTGCACCCCAGCCCCAACTGCAGCCTCCGAGC AGAGAACCTGGTCCACGTCCACTTCAAAGAGGAGATTGGCATTGCTGAGCTCATCCCGCTCGTGACCACC TACATCATCCTGTTTGCCTACATCTACTTCTCCACACGCAAGATCGACATGGTCAAGTCCAAGTGGGGCC TCGCCCTGGCAGCCGTGGTCACAGTACTTAGCTCACTGCTCATGTCTGTGGGGCTCTGCACCCTCTTCGG CCTGACGCCCACACTCAATGGCGGTGAGATCTTCCCATACCTGGTGGTCGTTATTGGGCTAGAGAACGTG TTGGTGCTCACCAAGTCAGTGGTATCAACTCCAGTGGACCTCGAGGTGAAGCTTCGGATTGCACAAGGCT TGAGTAGTGAGAGCTGGTCCATCATGAAGAACGCGGCGACCGAGCTGGGCATCATCCTCATTGGCTACTT CACCCTCGTGCCTGCTATCCAGGAGTTCTGCCTCTTTGCTGTTGTGGGCCTGGTGTCTGACTTCTTCCTC CAGATGCTGTTCTTCACCACTGTCCTGTCGATCGACATTCGCCGGATGGAGCTAGCAGACCTAAACAAGC GGCTGCCCCCTGAATCCTGCCTGCCCTCAGCCAAGCCCGTGGGGAGGCCAGCACGATATGAGAGACAGCA GGCTGTACGGCCATCCACGCCACACACCATCACATTGCAACCATCTTCCTTCCGAAACCTGCGGCTTCCC AAAAGGCTGCGTGTCATCTACTTCCTGGCCCGCACTCGCCTGGCCCAGCGCCTCATCATGGCTGGTACCG TTGTCTGGATTGGCATCCTGGTATACACCGACCCGGCAGGGCTGCGCACCTACCTTGCTGCCCAGGTGAC AGAGCAGAGCCCACTGGGTGAGGGTTCCCTGGGCCCCATGCCTGTGCCTAGCGGAGTGCTGCCTGCCAGC CACCCGGACCCTGCATTCTCCATCTTCCCACCTGATGCTCCTAAACTGCCAGAGAACCAGACCTTGCCAG GTGAGCTGCCTGAGCATGCTGGTCCAGCAGAGGGTGTCCATGACAGCCGAGCCCCAGAGGTAACTTGGGG GCTTGAGGATGAGGAGCTGTGGAGGAAATTGTCCTTCCGCCACTGGCCCACACTCTTCAACTACTACAAC ATCACACTGGCCAAAAGGTACATCAGCCTGCTGCCTGTCATCCCTGTCACACTACACCTGAATCCACGGG AGGCTCTGGAGGGGCGACACCCTCAGGATGGTCGCAGTGCCTGGGCCCCACAAGAGCCTTTGCCCGCTGG CCTCTGGGAGTCCGGACCTAAGGGACCAGGTGGAACACAGACCCATGGCGACATTACCTTGTACAAGGTG GCCGCGCTTGGCCTAGCAGCGGGCATCGTCCTGGTGCTGCTGCTGCTCTGCCTCTACCGGGTGCTCTGCC CGCGTAATTATGGGCAGCCGGGTGGTGGCCCCGGCAGGCGGAGGCGCGGGGAGCTGCCCTGCGATGACTA CGGCTACGCACCGCCCGAGACGGAGATAGTGCCGCTGGTGCTGCGAGGTCACCTCATGGACATCGAGTGT CTGGCTAGCGATGGGATGCTACTAGTGAGCTGCTGCCTGGCAGGCCAAGTCTGCGTGTGGGACGCTCAGA CAGGGGACTGCCTCACACGGATCCCACGCCCAGGGCCACGCCGGGATAGCTGCGGAGGTGGAGCTTTTGA GACTCAGGAGAACTGGGAAAGGCTGTCAGATGGAGGCAAGGCTAGCCCGGAAGAACCTGGAGACAGCCCT CCGCTGCGACGACGCCCCCGAGGGCCTCCACCGCCTTCCCTCTTTGGGGACCAGCCCGACCTCACCTGCT TAATCGACACCAACTTCTCGGTGCAGCTGCCCCCAGAGCCCACTCAGCCCGAGCCTCGGCACCGGGTGGG CTGTGGCCGCTCTAGAGACTCGGGTTATGACTTCAGCCGCCTGGTGCAGCGTGTGTACCAGGAGGAAGGC CTGGCTGCTATGCGCATGCCGGCCCTGCGCCCACCCTCCCCTGGACCTCCCTTGCCCCAGGCCTCTCAAG AAGAGGGGACTGCACCTGAGAAGGGCTCCCCTCCCCTGGCCTGGACCCCCAGCACAGCCGGTTCCATCTG GAGCTTAGAGCTGCAAGGCAATCTCATCGTGGTTGGGCGGAGCAGCGGCCGGCTGGAGGTGTGGGACGCC ATTGAGGGGGTGCTCTGCTGCAGCAATGAGGAGATCTCCTCAGGCATCACAGCCCTTGTCTTCTTGGACA GGAGGATTGTAGCTGCTCGGCTTAATGGTTCCCTTGATTTCTTTTCTTTGGAGACCCACACTTCCCTCAG CCCCCTGCAGTTCAGAGGGACCCCAGGGCGAGGCAGTTCTCCTTCCTCATCTGTGTACAGCAGCAGCAAC ACAGTGACCTGTCATCGGACCCACACAGTGCCCTGTGCACACCAGAAGCCCATCACAGCCCTGAGAGCTG CTGCCGGGCGCCTAGTGACAGGGAGCCAAGACCATACTCTAAGAGTCTTCCGACTGGATGACTCGTGTTG CCTCTTTACCCTGAAGGGCCACTCAGGGGCAATCACAGCTGTGTACATTGATCAGACCATGGTACTGGCC AGTGGAGGACAAGATGGAGCCATCTGCCTGTGGGATGTACTAACAGGCAGCCGGGTCAGCCAAACATTTG CTCACCGTGGAGATGTTACCTCCCTCACCTGTACCGCTTCCTGTGTCATTAGTAGTGGCCTGGATGACTT CATCAGTATCTGGGACCGCAGCACAGGCATCAAGCTGTACTCCATTCAGCAGGACCTGGGCTGTGGTGCA AGCTTGGGTGTCATCTCAGATAACCTTCTGGTGACCGGCGGCCAGGGCTGTGTCTCCTTTTGGGACCTAA ACTATGGGGACCTGTTACAGACAGTCTACTTGGGCAAGAACAGTGAAGCCCAGCCTGCCCGGCAGATTTT GGTGTTGGACAATGCTGCCATTGTCTGCAACTTTGGCAGTGAGCTCAGCCTAGTGTATGTGCCCTCTGTG CTGGAGAAACTGGACTGAAGGCAGGTCAAGTACGCTATTCCCTTTCCCCCATCCCAAGGTGGGGCACAGG GGATAGCAACTCTTTGGACCTAGACTAGAGGCAATAGCTGACTCTGAACTGTTGTCTCCTGACTGTAATA ATAAACTTTTTTAAAAAACCACAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | NM_001001144 |
| Insert Size | 3831 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC070437, AAH70437 |
| RefSeq Size | 4247 bp |
| RefSeq ORF | 3831 bp |
| Locus ID | 235623 |
| UniProt ID | Q6GQT6 |
| Gene Summary | Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211884 | Scap (tGFP-tagged) - Mouse SREBF chaperone (Scap) |
CNY 10,550.00 |
|
| MR211884 | Scap (Myc-DDK-tagged) - Mouse SREBF chaperone (Scap), transcript variant 1 |
CNY 8,888.00 |
|
| MR211884L3 | Lenti ORF clone of Scap (Myc-DDK-tagged) - Mouse SREBF chaperone (Scap), transcript variant 1 |
CNY 11,500.00 |
|
| MR211884L4 | Lenti ORF clone of Scap (mGFP-tagged) - Mouse SREBF chaperone (Scap), transcript variant 1 |
CNY 11,500.00 |
