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Pafah1b1 (NM_013625) Mouse Untagged Clone
CAT＃: MC205392
Pafah1b1 (untagged) - Mouse platelet-activating factor acetylhydrolase, isoform 1b, subunit 1 (Pafah1b1), transcript variant 1, (10ug)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | LIS-1; Lis1; Mdsh; MMS10-U; Ms10u; Pafaha |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC014831
GCGGAGTCCGGCGGCCGAGATAGCGAGTGAGGGAGCGAAGGAGCAGCGAGCCGAGAGTCCAGGGAGCGAG GAGGACCCGGAGGGGAGCGCTCAGGCGCGAGCGAGAGGACGAGCGAACGCTGGGCTAGGGCTCGAGCCCC GGAACGGCCGAGGAGCCCGCCCGCGGCCCTCCCCTCCCCCTCCCCGGGACCGGGCCCAGCGCGCCATCCT CCCCCTCCCTCCCTCCCTCCCTCCTTCCTCCCTTCCCTCTCCCTCCCCCTCCCCCGCTGGTGGATGGGAG TGAAGGACGGAAGAGGCCCTGCGGAGGCGGTGGTGCAGCGCTCCGGTGGAATGAACCTTACTTGTTGACT GGTTGCTGATTGGATTCACTTGAAAAAAAGAATCATTTTTCCCTCTGTGGAAGCCACATAGTGGCATATT TAATTCAAATCCAGGGATCACAACTTTTGATTTTTCTGGAGGGACATACCACTATATCAAATAAGCTTGA CATTACAGCCAAAATGGTGCTGTCCCAGAGACAACGAGATGAACTAAATCGAGCTATAGCAGATTATCTT CGTTCAAATGGCTATGAAGAGGCATATTCCGTTTTTAAAAAGGAAGCTGAACTAGATATGAATGAAGAAT TAGATAAGAAGTATGCTGGTCTTTTGGAAAAAAAATGGACATCTGTTATTAGATTACAAAAAAAGGTAAT GGAATTAGAATCAAAACTAAATGAAGCAAAAGAAGAATTTACGTCGGGTGGTCCTCTTGGTCAGAAACGG GACCCAAAAGAATGGATTCCCCGTCCACCTGAGAAATACGCATTGAGTGGTCATAGGAGTCCAGTTACTC GAGTCATTTTCCATCCTGTGTTCAGTGTTATGGTCTCTGCTTCAGAGGATGCTACAATTAAGGTGTGGGA TTATGAGACTGGAGATTTTGAGCGAACTCTCAAGGGCCATACAGACTCTGTACAGGACATTTCCTTTGAC CACAGTGGCAAGCTTCTGGCTTCCTGTTCAGCAGATATGACGATTAAATTATGGGATTTTCAGGGTTTTG AATGCATCAGAACCATGCACGGTCACGATCACAATGTCTCTTCAGTAGCCATCATGCCTAATGGAGATCA TATAGTGTCTGCCTCAAGGGATAAAACTATAAAGATGTGGGAAGTGCAAACTGGCTACTGTGTGAAGACA TTCACAGGACACAGAGAATGGGTACGTATGGTGCGGCCAAATCAGGATGGCACTCTGATAGCCAGCTGTT CCAATGACCAGACTGTGCGTGTGTGGGTTGTAGCAACAAAGGAATGCAAGGCTGAGCTCCGAGAACATGA ACATGTGGTGGAATGCATTTCCTGGGCTCCAGAAAGTTCATATTCTTCCATCTCTGAAGCAACAGGATCT GAGACTAAAAAAAGTGGCAAGCCTGGACCTTTCTTGCTATCTGGTTCCAGAGACAAAACTATTAAGATGT GGGACGTCAGTACTGGCATGTGCCTTATGACTCTTGTGGGTCATGATAACTGGGTACGTGGAGTTCTGTT CCATTCTGGGGGGAAGTTTATTTTGAGTTGTGCTGATGACAAGACCCTCCGTGTATGGGATTATAAGAAC AAGCGATGCATGAAGACCCTCAATGCGCATGAACACTTTGTTACCTCCTTGGATTTCCATAAGACGGCAC CCTATGTGGTTACTGGCAGTGTAGATCAAACAGTAAAGGTGTGGGAGTGCCGTTGATTGAGTCACATTTG GTTCTTCCTCCCTTTTTTCCTCTGGATGCACTCTGATGATACCATGGTTACCCCATTTGAGCTCTGTTTA AATAAATATTGTCCTTTCATGTAAATTATTCTGGATGTAGATTGAACTTATTAAATGTTACACACAAAGT ATTCATGCATGGTGAATCCAAATTGTATACTGTAAATTTACATATGTTGTCTAGAAGTACCATAGGGTTT AAAAACCTGGGCTGGCATTGGTCACACCAGGCCTACAAGGGCAGAAGTTAAATAAATTGAACTAGGGCAC TAAACTGAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_013625 |
| Insert Size | 1233 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC014831, AAH14831 |
| RefSeq Size | 2052 bp |
| RefSeq ORF | 1233 bp |
| Locus ID | 18472 |
| UniProt ID | P63005 |
| Gene Summary | Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. Also required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position. Required for dynein recruitment to microtubule plus ends and BICD2-bound cargos (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes a functional protein. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR206450 | Pafah1b1 (Myc-DDK-tagged) - Mouse platelet-activating factor acetylhydrolase, isoform 1b, subunit 1 (Pafah1b1), transcript variant 1 |
CNY 5,488.00 |
|
| MR206450L3 | Lenti ORF clone of Pafah1b1 (Myc-DDK-tagged) - Mouse platelet-activating factor acetylhydrolase, isoform 1b, subunit 1 (Pafah1b1), transcript variant 1 |
CNY 5,990.00 |
|
| MR206450L4 | Lenti ORF clone of Pafah1b1 (mGFP-tagged) - Mouse platelet-activating factor acetylhydrolase, isoform 1b, subunit 1 (Pafah1b1), transcript variant 1 |
CNY 5,990.00 |
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